- NEPA21 Electroporator- ELEPO21 Electroporator- NEPA Porator- ECFG Porator- Transgenic Mouse Zygote Electroporation: NEPA21- Organoid Electroporation: NEPA21- Neuroscience & Genome Editing Publications- Hybridoma Production for Monoclonal Antibodies- Bacteria / Yeast Electroporation- SP100: Sonoporator- NEPA21: Illustrated Applications- NEPA21: Publications by Research Application- Connection Cables - CU700: Monitoring System- CU902: Polarity Exchanger- CUY21 EDIT Electroporator - CUY21 EDIT-S Electroporator - CUY21 SC Electroporator- In Vivo, In vitro Electrodes- Electroporation Cuvettes- Cuvette Chamber & Stand Holder- Laser Thermal Microinjector- Mechanical Vibration Units- Ultrasounic BioMicroscope- KTAC-4000: Sonoporator- LF101: Cell Fusion Device- LF201: Cell Fusion Device- Electro Cell Fusion Electrodes- Electroporation and
Electro Cell Fusion Accessories- Electroporators- Publications/Protocols- Electrodes by Product Codes
- Mouse : Muscle / Brain / Skin / Liver / Retina / Kidney / Bladder / Ovary - Rat : Brain / Knee joint / Retina / Kidney / Liver / Skin / Bladder / Ovary - Zebrafish : Fin / Retina - Honeybee : Brain - Planerian : Eye - Xenopus : Embryo
- Mouse & Rat Embryo : Spinal Cord / Hippocampus / Cortex / Telencephalon, etc - Newborn Mouse & Rat : Retina / Cornea
- Chick Embryo : Neural tube / Lim bud / Mesencephalon / Optic vesicle, etc
- Mouse brain slice / Mouse embryo / - Mouse embryo gonad / Mouse small intestine, etc
- Adherent Cell / Plant Protoplast
The CUY21SC electroporator is desigend specifically for sensitive in vivo, in ovo, in utero and ex vivo electroporation.
Because in vivo impedance (electrical resistance) is generally less stable than in vitro
impedance, an accurate measurement of the target sample resistance
prior to the electroporation event is the most important critical
success factor for accurate and reproducibe electroporation.
Fluctuations in resistance impact on
delivered current values and this negatively impacts reproducibility
from one EP event to the next. The
CUY21SC automatically measures and displays the impedance of the target sample. (For example, the
resistance of an EP target varies according to physical experimental
factors such as the volume of the sample, the buffer used and the
distance between the electrodes). In order to assure delivery of
the optimal current, the researcher needs to ascertain the actual
resistance of the target sample prior to the EP event. The CUY21SC
function enables the researcher to check the sample impedance prior to
running the EP program and adjust it if the recorded impedance value
deviates from the previously recorded value. This ensures
reproducibility from one EP event to the next.
Consistent delivery of accurate electric current is the primary factor contributing to successful electroporation results. Not only does the CUY21SC electropator automatically measures and display delivered current
information, but it can contorl and measure delivered current from the low ampere (1mA) up to 1.60A. The CUY21SC was developed to enhance the delivery of genes into sensitive targets such as Mouse and Rat: Brain, Embryo, Tissue Slice and Chick Embry. For such and similarly sensitive targets, delivery of a controlled and low current is essential to avoid damage to the sensitive cell or brain targets. The CUY21SC makes it possible to control the delivery of low current and automatically measures that delivery. Access to accurate output current information will assist
researchers optimise their electroporation experiments and achieve
consistence and reproducible results. Ability to deliver controlled, sensitive (low) currents will minimise damage to sensitive targets and increase transfection efficiency.
The CUY21SC was designed to ouput precise, low-voltage, square wave pulses that do not degrade over the course of the pulse. Accurate and low-voltage square wave pulses ensure
higher transfection efficiency and cause less damage to (especially sensitive) electroporation targets. (Indeed, we have found that with the CUY21SC successful gene delivery into single cell/specific and
sensitive targets is optimised in the 1V or 2V range). With the CUY21SC
the voltage can be set from 0.1 to 99.9V in 0.1V increments. This
enables precise voltage control and precise current (below 10mA)
measurement. The low voltage range of the CUY21SC enables it to cover almost ALL in vivo, in utero, in ovo and ex vivo applicaitons. This wide application-range is not something other electroporators offer and is an important considering when making a purchasing decision for your laboratory.
Over 250 different electrode configurations have been designed and optimised for use with the CUY21SC
electroporator. Such an extensive electrode range extends the
application range of the CUY21SC device. This is another important
consideration at the point of purchase, as researchers have to be
mindful that they may, in the future, wish to expand their field of
interest to include other electroporation applications. Furthermore,
custom-made electrodes can be easily designed and manufactured to suit
your particular application of interest.
output voltage can be measured after the electroporation event. By
checking the post EP event voltage, it is easy to determine whether the
programmed voltage of the DC pulse was generated. This is a useful
cross-check to establish if something malfunctioned with the device.
performing in vivo electroporations some researchers hold the
electrodes by hand and as the conductive part of the electrode is
exposed there is a potential safety risk of accidental electrocution.
To prevent accidental electrocution, the CUY21SC has an integrated
safety limit function. The electroporation program automatically shuts
down when the current reaches its excess limit (1.60A).
seven independent LED displays (for each of the electroporation
parameters) enable program settings and measured values to be checked at
a glance. For example, measured voltage and current are displayed
immediately after electroporation. Separate numerical keys also make it
easy for the operator to edit a saved program or set a new one.
Number of Pulses
0.1-99.9V (0.1V resolution)
0.05-99.9ms (0.01ms resolution)
0.1-999.9ms (0.1ms resolution)
Current: 1-999mA (1mA resolution)
.. 1.00-1.60A (0.01A resolution)
W360 x L380 x H180, 12.3Kg
115V or 220-240VV, 50/60Hz
* Safety function: When current exceeds the limit the electroporation program is automatically shut down.
In addition to the precise current control offered by the CUY21 system, the CUY21 system also delivers a precision Square Wave throughout its pulse (unlike the decaying square wave pulses delivered by competing devices)
During electroporation, DNA naturally moves to and accumulates at the
positive (+) electrode pole (similar to the process in
electrophoresis). However, such accumulation (if not addressed)
adversely impacts on electroporation transfection efficiency. The CU902
Polarity Exchanger addresses the sub-optimal effects of accumulation by
reversing the electrical polarity of the electrodes during the
electroporation event, thus preventing accumulation and ensuring better
DNA uptake throughout the whole of the cells.
Mesencephalon (HH* stage 10)
Diencephalon (HH* stage 10)
Head Ectoderm (HH stage 10)
Lens (HH stage 10)
Lateral Plate Mesoderm (LPM)
Somitic Mesoderm (HH stage 7-9)
Limb Bud (HH stage 7-9)
Digestive Organ (HH stage 7-9)
Ventricle (HH stage 30)
Retina （HH stage 38-42）
CUY614 & CUY615
CUY613 series CUY614 & CUY615
Neural Plate (HH stage 3-7)
Cerebral Cortex, All Area (> E13.5)
Cerebral Cortex, Specific Area (> E13.5)
Forebrain Ventricle (E14)
Spinal Cord (E10.5)
Neural Plate (Mouse: E7-13
Metencephalon (Mous: E7-13)
Somite (Mouse: E7-13）
Telencephalon (Mouse: E7-13）
CUY520P15, P20, P25
CUY650P3, P5, P10
Anterior Tibial Muscle
CUY650P3, P5 (Holding)
CUY250 series combination
*CUY21SC is recommended.
Brain Ventricle (P4-P8)
*CUY21SC is used.
We stock over 250 different original electrode configurations
specifically designed for use with the CUY21 electroporator range.
This significantly expands the experimental applications range of our
We also offer a customized electrode design service. We can design and manufacture electrodes to our particular research requirements.
Please feel free to contact us
you see an electrode you want more information on, please enter the
product code (e.g., CUY650P5) into the 'Search by product code, name or
application' search box on the top left hand side of the webpage and
click on the 'Search' button.
CUY21 Illustrated Applications
CUY21 Electrode Recommendations
CUY21 Electroporation Publications - Full List
CUY21 Electroporation Publications - by Research Interest
CUY21SC Square Wave Electrop