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Featured Device

0
Product Code: SONIDEL SP100

Desc:Sonoporator
Applic:(hover for application)Application:
  • Delivery of plasmids to cells and tissues for gene therapy-based applications and studies.
  • Delivery of nucleic acids such as siRNA, RNAi etc. to cells and tissues for studies on control of gene expression/gene therapy.
  • Delivery of cancer chemotherapeutic agents to impermeable target cells/tissues.
  • Delivery of agents to cells to study metabolic effects.


Featured Electrode



Product Code: CUY260-0.8

Application:
Electrodes for Electroporation
Description:
Stainless Steel, Needle electrode for endoscope, 0.8mmφ

Information
- How to order
- Contact us

NEPA Porator for E Coli

Ohm Checkable Exponential Decay Wave Electroporator

NEPA Porator

Transformation / Transfection Applications

  • E. coli
  • Bacterial cells
  • Yeasts
  • Fungi
  • Mammalian cells

Ohm check for controlling electroporation

Ohms Law
Electroporation is generated by delivering an electric pulse(s), and the delivery of the electric pulse(s) at the same voltage and the same current with the same resistance under the ohm’s law is essential for achieving highly reproducible results.

The NEPA Porator can measure the electric impedance (that is almost equal to resistance) of the sample between the electrodes. Checking the impedance prior to electroporation is greatly helpful for avoiding inconstant electroporation, reviewing electroporation results and performing expected electroporation that will bring desired transformation results.

スペース
Voltage setting; 500 V
Checked sample impedance: 36 ohms
Voltage setting; 500 V
Checked sample impedance: 3,909 ohms
36ohms 3.9kohms
Output electric pulse
measured by oscilloscope
Output electric pulse
measured by oscilloscope

Specifications

Settings of exponential decay wave pulse

Voltage 100–500 V (5 V resolutionn)
500–3,000 V (100 V resolution)
Pulse length None setting or 1.0-5.0 msec (0.5 msec resolution)
Number of pulses 1 or 2
NEPA Porator exponential decay wave pulse
*Pulse length setting: none
NEPA Porator exponential decay wave pulse
*Pulse length setting: 1.0-5.0 msec

Measurements of output pulses

Voltage 100–3,000 V (1 V resolution)
Time constant None setting: Measured value is displayed
1.0-5.0 msec setting: Set pulse length value is displayed
Number of pulses 1 or 2

Others

Impedance Measurement 15–9,999 Ω (1 Ω resolution)
10,000–50,000 Ω (10 Ω resolution)
Save 99 programs
Dimensions 335 (W) x 205 (D) x 85 (H) mm
Weight 3.2 kg

*All features and specifications subject to change without notice.


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