NEPA21 Electroporator

Super Electroporator NEPA21

The NEPA21 Electroporator is our latest and most sophisticated electroporation device to-date. It is the successor to our hugely successful CUY21EDIT and CUY21SC electroporator range.

The NEPA21 Electroporator is based on the same platform and core technology that made the CUY21EDIT and CUY21SC devices leaders in their class. The NEPA21 can do in one device what each of the EDIT and SC did individually, i.e., it combines BOTH the wider voltage range of the EDIT and the enhanced sensitivity at lower voltages of the SC.

The NEPA21 has been bench-marked and tested against the CUY21 series to ensure that it delivers the exact same protocol results as the EDIT and SC devices for each and every published application. In addition, the NEPA21 also offers other novel attributes that extend its application range even further:

- a 2-Step Pulse: A Electroporation (Poring) Pulse (a high voltage & short duration pulse) and a Transferring (Driving) Pulse (a low voltage with multiple duration settings pulses)

- the NEPA21 not only incorporates the full In Vivo, In Utero, In Ovo and Ex Vivo application ranges of the CUY21EDIT and CUY21SC devices, but it also includes a completely new and novel In Vitro application range

- it can be targeted at BOTH In Vitro (Suspended Cells and Adherent Cell) and In Vivo applications

- lower operating-cost advantages for both Suspended Cells and Adherent Cells (in commercially available cell culture plates) and In Vivo applications (there is no requirement for expensive special reagents)

- delivers High Transfection Efficiency and Viability

- a voltage output-range that matches the published extended-output voltage protocols of the CUY21EDIT device

- an enhanced voltages output-control that matches the renowned sensitivity of the CUY21SC device at low voltage settings

NEPA21 Square Wave Electroporator

In Vitro & In Vivo Transfection

NEPA21 Square Wave Electroporator

High Transfection Efficiency & Viability WITHOUT Special Buffers

Features:

In Vitro Transfection

For PRIMARY CELL, STEM CELLS and ANY CELL Line:

- With Electroporation Cuvettes For Cells in Suspension

- With Adherent Cell Electrodes For Cells in Adherence

In Vivo Transfection

For MOUSE/RAT BRAIN, EYE, MUSCLE, SKIN, LIVER, KIDNEY, TESTIS, ETC

In Utero Transfection

For MOUSE/RAT EMBRYO:

- CEREBRAL, CORTEX, HIPPOCAMPUS, SPINAL CORD, ETC

In Ovo Transfection

For CHICK EMBRYO:

- NEURAL TUBE, LIMB BUD, DIGESTIVE ORGAN, ETC

Ex Vivo Transfection

For TISSUE SLICE, BRAIN SLICE, ORGAN, WHOLE EMBRYO, ETC

The NEPA21 Electroporator covers the full application range of its predecessor CUY21EDIT and CUY21SC electroporators

The NEPA21 Electroporator complies with the CE Certification, UL standard and CSA standard.

Request a Demo

And see how the NEPA21 performs with your Cell Lines

Novel 3-Step Multiple Electroporation Pulse:

	The 3-Step pulse regime with voltage decay and polarity exchange facilitates more highly controlled electroporation pulse delivery. This results in higher transfection efficiency and higher cell viability WITHOUT a requirement for specialised buffers. .
	Step 1	Membrane ‘Poration’ Pulse Mode:High Voltage, Short Duration, Single/Multiple Pulse(s), Voltage Decay This poration pulse forms pores in the cell membrane with minimum residual cell damage .
	Step 2	Transfer Pulse Mode:Low Voltage, Long Duration, Multiple Pulses, Voltage Decay. This transfer pulse is for electrokinetic delivery of the target molecules (DNA, RNA, etc.) into cells with minimum residual cell damage .
	Step 3	Polarity Exchanged Transfer Pulse This can improve the transfection efficiency and again, minimise residual cell damage .

Applications with Electroporation Cuvettes

The NEPA21 Electroporator makes it possible to achieve high transfection efficiency and viability without recourse to special buffers for PRIMARY CELLS, STEM CELLS and difficult-to-transfect cells as IMMUNE CELLS, BLOOD CELLS, ETC.

Click here for further Electroporation Cuvette information

1) Transfection into Primary Cells

- BMMC Primary Mouse Bone Marrow-Derived Mast cells

Viability: 64%	Transfection Efficiency: 78%
. FACS Data
. .
..Our transfection efficiency data is a ratio of transfected cells to survived cells. (E.g. If the original cells are 10, viable cells are 8 and transfected cells are 6, the viability is 80% (8/10) and the transfection efficiency is 75% (6/8).)
. - Primary Mouse Neurons, Cerebral Cortex

Viability: 80%	Transfection Efficiency: 70%

. - Primary Rat Bulbar Nerve Cells

Viability: 80%	Transfection Efficiency: 75%

. - HASM Primary Human Airway Smooth Muscle cells

Viability: 90%	Transfection Efficiency: 80%

. - Primary Human Endometrial Stromal Cells

Viability: 95% .	Transfection Efficiency: 90% .

Disposable Kit Comparison

NEPA21 (Nepa Gene)	Device: N (Company L)	Device: N (Company I)
Cuvettes ONLY No Special Buffers	Transfection Kits With Special Buffers	Transfection Kits With Special Buffers
Cost per One Sample Euro 1.80 (€90.00/50 pcs)	Cost per One Sample Euro 18.60 (€186.05/10 RCT)	Cost per One Sample Euro 15.00 (€375.00/25 Tips)
*Euro Price.

.Is your laboratory still using a transfection device that requires expensive disposable kits?

Many of our clients cite the NEPA21′s lower running cost as a major factor in deciding to purchase the device.

The running cost of NEPA21 is much lower than other transfection devices.

2) Transfection into Cell Lines

(V: Viability, TE: Transfection Efficiency)

Please click the following links for the corresponding Cell Image.

Cell Line		V	TE	Cell Line		V	TE
	Species: Human			Raji	Human Burkitt’s Lymphoma cells	74%	53%
HeLa	Human Cervical Carcinoma cells	95%	95%	LCL	Human Lymphoblastoid cell	55%	43%
293	Human Embryonic Kidney cells	90%	90%	Nalm-6	Human B-cell Precursor Leukemia cells	65%	63%
293T	Human Embryonic Kidney cells	90%	90%	KU812	Human basophilic leukaemia cells	96%	42%
TIG-7	Human Embryonic Lung Fibroblasts	89%	76%	MutuⅠ	Human Burkitt Lymphoma cells	87%	91%
MRC-5	Human Embryonic Lung Fibroblasts	85%	90%	MutuⅢ	Human Burkitt Lymphoma cells	54%	92%
SUSM-1	Human Fibroblasts	77%	71%
KMST-6	Human Fibroblasts	70%	60%		Species: Mouse
HT1080	Human Fibrosarcoma cells	93%	81%	MEF	Mouse Embryonic Fibroblasts	80%	90%
MIA-PaCa-2	Human Pancreatic Carcinoma cells	80%	77%	L	Mouse L cells	90%	65%
HepG2	Human Hepatoma cells	80%	76%	MC3T3-E1	Mouse Osteoblastic cells	40%	80%
HuH-7	Human Hepatoma cells	70%	60%	MS-1	Mouse Pancreatic Endothelial cells	90%	90%
H1299	Human Lung Cancer cells	90%	90%	RAW264.7	Mouse Macrophage-like cells	70%	56%
HSC-2	Human Squamous Carcinoma cells	93%	98%	MIN6	Mouse Pancreatic Beta cells	57%	71%
HSC-3	Human Squamous Carcinoma cells	93%	98%	MEL	Mouse Erythroleukemia cells	70%	50%
OVCAR-3	Human Ovarian Carcinoma cells	90%	79%	XS106	Mouse Dendritic cells	61%	45%
RMG-1	Human Ovarian Carcinoma cells	97%	67%	mDC	Mouse Myeloid Dendritic cells	79%	72%
TE-1	Human Esophageal Carcinoma cells	85%	41%	MC/9	Mouse Mast cells	76%	84%
TE-8	Human Esophageal Carcinoma cells	85%	40%	TS	Mouse Trophoblast Stem cells	59%	47%
MCF-7	Human Breast Cancer cells	90%	93%
T47D	Human Breast Cancer cells	90%	85%		Species: Rat
NUGC-3	Human Gastric Carcinoma cells	73%	68%	PC12	Rat Adrenal Pheochromocytoma cells	70%	50%
A549	Human Lung Adenocarcinoma cells	85%	90%	H9c2	Rat Ventricular Myoblasts	71%	82%
LNCaP	Human Prostate Carcinoma cells	71%	90%	REF	Rat Embryonic Fibroblasts	90%	99%
RKO	Human Colorectal Carcinoma cells	80%	40%	C6	Rat Glioma cells	80%	67%
SK-N-SH	Human Neuroblastoma cells	95%	95%
SH-SY5Y	Human Neuroblastoma cells	60%	90%		Species: Hamster
KG-1-C	Human Oligodendroglial cells	85%	60%	CHO	Chinese Hamster Ovary cells	74%	90%
HaCaT	Human Keratinocyte cells	40%	80%	CHO-K1	Chinese Hamster Ovary cells	95%	95%
CCD1079sk	Human Skin Fibroblasts	40%	53%
iHAM-4	Human Amniotic Mesenchymal cells	59%	95%		Species: Chicken
iHAM-7	Human Amniotic Epithelial cells	70%	40%	DT40	Chicken B cells	38%	40%
HTR-8/Svneo	Human Trophoblast cells	95%	67%
Jurkat	Human T-cell Leukemia cells	71%	73%		Species: Carp
Namalwa	Human Burkitt’s Lymphoma cells	70%	75%	GTS9	Ginbuna Thymus cells	50%	30%

NEPA21 Cell Image Results

NEPA21 Cell Transfection Data

**Applications with Adherent Cell Electrodes**

By using the Adherent Cell Electrode CUY900 series, it is now possible to transfer DNA/RNA directly into CELLS IN ADHERENCE in commercially available 24-well plates.

● Gene Transfection into Primary Neurons (Adherent) by Electroporation

Confirmation of ECM830 Delivered Voltage at 100V

Confirmation of CUY21SC Delivered Voltage at 100V

pCAGGS-EGFP plasmid was transferred into primary neurons cultured for 6 days in adherent state.

The neurons were prepared from E15 mouse embryo cerebral cortex.

A): .2-step pulse electroporation using the electrodes for adherent cells (CUY900-13-3-5)

B): .EGFP fluorescence image of the neurons 2 days after electroporation

C): .High magnification image of Figure B

…....Many robust EGFP signals suggest high transfection efficiency.

D): High magnification image of Figure C (x40)

…....Neurites are shown clearly

Department of Neurochemistry, National Institute of Neuroscience

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Applications with In Vivo Electrodes

. NEPA21 electroporator together with its specially designed CUY-series in vivo electrode range is optimised to facilitate the transfer of DNA/RNA into:

.………………….MOUSE/RAT Muscle, Skin, Liver, Kidney, Testis, Ovary, Brain, Retina, Cornea, Etc.

DNA-RNA transfer into MOUSE-RAT MUSCLE, SKIN, LIVER, KIDNEY, TESTIS, OVARY, BRAIN, RETINA, CORNEA

………….MUSCLE…………………………………….SKIN……………………..TESTIS

…………………..RETINA…………………..BRAIN……………………EGG

Please click here for further DETAILED APPLICATION INFORMATION

Please click here for further ELECTRODE INFORMATION

Please click here for NEPA21/CUY21 Publications

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Applications withIn Utero/Ex Utero Electrodes

.The NEPA21 electroporator together with its specially designed CUY-series In Utero/Ex Utero electrode range is optimised to facilitate the transfer of DNA/RNA into:.

……………………MOUSE/RAT Embryo (Cerebral Cortex, Hippocampus, Spinal Cord, Etc.)