Adherent Cells
Electrode Recommendation
Previously, when clients requested an electrode recommendation for a 35mm diameter dish we suggested the CUY510 electrode.
However, as a result of client feedback we now know that this type of electroporation can be performed (much more successfully) on the entire dish using the:
-Â Â Â Â Â Â Â Â Â CUY512-5
-Â Â Â Â Â Â Â Â CUY513-4 and
-Â Â Â Â Â Â Â Â CUY513-5 electrodes
Download Technical Drawing of CUY512 and CUY513 electrodes and Electric Field
Electroporation Parameters
| Model | Electrode | Cell | Voltage | Pulse length |
Pulse interval |
No. of Pulses | Optimal current |
Note | |||||||
| CUY21EDIT | CUY513-5 | Neuron Cell | 110V | 5msec | 99msec
or 95msec |
5 pulses or   5 pulses, (reverse polarity) | 0.15A | 50-70% confluent Hiporation buffer is added. |
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| CUY21EDIT | CUY513-5 | Hippocampal Neuron Cell | 110V | 2msec | 98msec | 5 pulses 5 pulses (reverse polarity) |
0.15A | 50-70% confluent | |||||||
| CUY21EDIT | CUY512-5 | Fibroblast Cell | 300V | 5msec | 95msec | 3 pulses 3 pulses (reverse polarity) |
0.15A | Efficiency of gene transfection : 30% Viability : 60% |
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| CUY21SC | CUY512-5 | Muscle Cell | 80-90V | 10msec | 90msec | 10 pulses 10 pulses (reverse polarity) |
0.17A | 50-70% confluent When current < 170mA, efficiency of gene transfection is low. When current > 200mA, cells are peeled off. |
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| CUY21EDIT | CUY513-5 | Hela Cell | 7-14V | 60ms | 50ms | 4 pulses 4 pulses (reverse polarity) |
N/A | Peptide transfection | |||||||
Kindly note the following article:
- ‘Expression Regulation of Hyaluronan Synthase in Corneal Endothelial Cells’