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Featured Device

0
Product Code: SONIDEL SP100

Desc:Sonoporator
Applic:(hover for application)Application:
  • Delivery of plasmids to cells and tissues for gene therapy-based applications and studies.
  • Delivery of nucleic acids such as siRNA, RNAi etc. to cells and tissues for studies on control of gene expression/gene therapy.
  • Delivery of cancer chemotherapeutic agents to impermeable target cells/tissues.
  • Delivery of agents to cells to study metabolic effects.


Featured Electrode



Product Code: CUY5000P1

Application:
Electrodes for Electro Cell Fusion
Description:
Platinum wire electrodes on petridish, 1mm gap

Information
- How to order
- Contact us

Adherent Cells

Adherent Cell Electroporation Applications

Cell Culture Transfection

Transfection in Cell-Culture-Plates/Dishes

It is now possible to transfer DNA/RNA directly into cells ADHERENT CELLS in commercially available multi-well plates.  We recommend the use of our CUY900 series electrodes

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Cells

V

TE

Cells

V

TE

Species: Human

Primary Mouse Hippocampal Neurons
(Embryonic day 14) (4 DIV)

60%

50%

Primary Mouse Hippocampal Neurons
(Embryonic day 18) (2 DIV)

85%

54%

Primary Mouse Microglial cells
(1 DIV after 1 week co-culturing astrocyte and microglial cells)

80%

73%

Primary Mouse Glial cells
(14 DIV)

80%

50%

Primary Mouse Stromal cells
(1-month cultured)

90%

50%

Primary Rat Cerebral Cortex Neurons
(Embryonic day 17) (2 DIV)

70%

60%

Primary Rat Hippocampal Neurons
(Postnatal day 7) (11 DIV)

100%

50%

Rat Granulosa cells

Excel.

41%

SH-SY5Y Human Neuroblastoma cells

90%

50%

EPC Human Endothelial Progenitor Cells

3T3-L1 Mouse Embryonic Fibroblasts
(7 days after differentiation)

90%

70%

C6 Rat Glioma cells

57%

55%

Transfection into Primary Neurons

CUY900-13-3-5 electrode in well plate
Click to enlarge

EGFP fluorescence image of the neurons 2 days after electroporation
Click to enlarge

Magnified view.jpg
Click to enlarge

High magnification image of Figure C (x40). (Neurites are clearly visible).
Click to enlarge

pCAGGS-EGFP plasmid was transferred into primary neurons cultured for 6 days in an adherent state.
The neurons were prepared from E15 mouse cerebral cortex.

A):

2 steps pulse electroporation using our CUY900-13-3-5 electrode

B):

EGFP fluorescence image of the neurons 2 days after electroporation

C):

High magnification image of Figure B. Many robust EGFP signals suggest high transfection efficiency.

D):

High magnification image of Figure C (x40). (Neurites are clearly visible).

Data provided courtesy of Department of Neurochemistry, National Institute of Neuroscience, Japan

We have a lot of adherent-cell transfection data demonstrating high efficiency and high viability. Please feel to free to contact us for further details.

CUY900-13-3-5 Technical Drawing

 

 

EP Conditions for single-pulse CUY21 series electroporators

(Contact us for the relevant NEPA21 multi-pulse (with polarity exchange) EP Conditions)

Model Electrode Cell Voltage Pulse
length
Pulse
interval
No. of Pulses Optimal
current
Note
CUY21EDIT CUY513-5 Neuron Cell 110V 5msec 99msecor95msec 5 pulses or   5 pulses, (reverse polarity) 0.15A 50-70% confluent
Hiporation buffer is added.
CUY21EDIT CUY513-5 Hippocampal Neuron Cell 110V 2msec 98msec 5 pulses
5 pulses (reverse polarity)
0.15A 50-70% confluent
CUY21EDIT CUY512-5 Fibroblast Cell 300V 5msec 95msec 3 pulses
3 pulses (reverse polarity)
0.15A Efficiency of gene transfection : 30%
Viability : 60%
CUY21SC CUY512-5 Muscle Cell 80-90V 10msec 90msec 10 pulses
10 pulses (reverse polarity)
0.17A 50-70% confluent
When current < 170mA, efficiency of gene transfection is low.
When current > 200mA, cells are peeled off.
CUY21EDIT CUY513-5 Hela Cell 7-14V 60ms 50ms 4 pulses
4 pulses (reverse polarity)
N/A Peptide transfection

Kindly note the following article:

‘Expression Regulation of Hyaluronan Synthase in Corneal Endothelial Cells’

 


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