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Featured Device

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Product Code: SONIDEL MB101

Desc:Microbubble for Ultrasound-mediated Transfection
Applic:Sonoporation Aid


Featured Electrode



Product Code: CUY531P0.5

Application:
Electrodes for Electro Cell Fusion
Description:
50mm length & 3mm height platinum blocks on petridish, 0.5mm gap

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- How to order
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Top » Product Catalogue » Cell Fusion Generators » LF101 »

LF101 - Electro Cell Fusion Generator

Applications

  • Bovine
  • Pig
  • Mouse and Rabbit Cloning
  • Somatic Cell and Embryo Nuclear Transplant

LF101 ElectroCell Fusion Generator

  • Plant Protoplast Fusion
  • Mamallian Cell Fusion



Cell Fusion

Nuclear Transfer (Cloning)



Electro Cell Fusion Advantages v PEG


  • Greater control on the fusion
  • Double/triple efficiency compared with PEG
  • Electro cell fusion results are more stable
  • No Toxicity
  • Reproducibility not dependant on Researcher Skill

Features

 

Increased Reproducibility

To ensure accurate and reproducible cell fusion it is essential to align the target cells prior to the fusion event. If the ion concentration of the bufferis too high, ions will cause a water stream which will disturb the cell alignment (pearl chain). Uniquely, the LF101 enables the researcher tomeasure the resistance of the buffer prior to cell fusion and ensure the
correct ion concentration for exact cell alignment (in all experiments). This greatly enhances reproducibility.

Large Choice of Electrode Configuration

A large selection of possible electrode configurations available.  The electrodes have been specifically designed for the LF101 device. 

Pulse Characteristics

Both automatic and manual pulse delivery (number of pulses 1-99) modes avaailable.  Pulse interval can be set in increments from 0.1 - 9.9 seconds.

 

Voltage Settings

Voltage can be set in 1V resolutions.  This is especially suitable for mammalian cloning.  The device outputs a precise square waves which enhances cell fusion probability.  

Ease of Operation

For ease of operation, each cell fusion parameter is displayed on its own
individual LED.

 

 

DC Pulse Output

The LF101 enables the researcher to output the DC pulse either automatically or manually. The automatic output mode facilitates routinework. The manual output mode enables the researcher to apply a DCpulse while using a microscope - this is particularly useful when working with unfamiliar cells and during nuclear transplantation.

Somatic Cell & Embryo Nuclear Transplant

Specialized setting for bovine, pig, mouse, rabbit cloning.  Somatic cell and embryo nuclear transplant results in extremely high fusion probability (e.g., more than 90% for bovine cloning).

Plant Protoplast & Mammalian Cell Fusion

Specialized setting for plant protoplast and mammalian cell fusion provide optimal cell fusion conditions.  Cell fusion achieved which a DC pulse (100V, Max) of precisely controlled voltage and pulse range after producing the pearl chains with DC. It can elevate the rate of hybridoma production (Cell fusion) probability.

 

 

 



 

Specifications


AC Pulse

Wave Shape

Sine Wave

Voltage

0 - 50V (VP-P)

Frequency

0.1 - 3.9MHz Variable

Duration

0 - 99s

Shunt Resistor

Greater than 50 ohm


-  Power : 100V (50/60Hz) 2A
-  Size : 370W x 430D x 160H mm
-  Weight : 10kg

 

 

DC Pulse

Wave Shape

Square

Voltage

0 - 999V
0 - 200V (1V resolution)*

Pulse Length Range

5 - 99ms (1ms resolution)

Number of Pulses

1 - 99 Automatic or Manual

Pulse interval

0.1 - 9.9s

Shunt Resistor

Greater than 50 ohm

Post fusion

Switched On/Off


*Voltage for Cloning: 0 - 200V

 

 


Applications and Electrode Selection : Cell Fusion

Applications and Electrode Selection

Nuclear Transfer (Cloning) and Oocyte Activation

Nuclear Transfer

 

 

 

 

 



 

LF101

LF101 Specifications:

LF101 - Electro Cell Fusion Generator - Nuclear Transfer

  

Applications

Bovine, Pig, Mouse and Rabbit Cloning (Somatic Cell and Embryo Nuclear Transplant)

Plant Protoplast Fusion 

Mammalian Cell Fusion

 



Features

Specialized settings for bovine, pig, mouse, rabbit cloning (Somatic cell and embryo nuclear transplant) result in extremely high fusion probability (for example: More than 90% for bovine cloning).

Voltage setting with 1V resolution is possible in the specialized setting for mammalian cloning. It is suitable to output precisely the square pulse wave shape and pulse range as preset and accordingly brings about a higher rate of cell fusion probability.

Specialized setting for plant protoplast and mammalian cell fusion can provide a desirable condition for the cell fusion, which is done with DC pulse (100V, Max.) of the precisely controlled voltage and pulse range after producing the pearl chains with DC. It can elevate the rate of hybridoma production (Cell fusion) probability.

•    Post fusion function for damage recovery of the cell can be operated with On/Off switching.

•   Operation of the device is very easy because both of automatic and manual (Number of pulses: 1-99) transmitting of the pulse are possible. And the pulse interval can be selected to set between 0.1-9.9 seconds. The device can keep a constant operating condition anytime.

A comprehensive selection electrodes designed specifically for the device is available.  Using the specifically designed electrodes with the device facilitates very efficient gene transfection and cell fusion.

 

Specifications

AC

DC Pulse

Wave Shape

Sine Wave

Wave Shape

Square Wave Pulse

Voltage

0-50Vrms, 1V resolution

Voltage

0-920V

0-200V (1V resolution)

Frequency

0.1-3.9MHz Variable

 

  

Duration

0-99s (1sec resolution)

Pulse Length

5-99μs (1μsec resolution)

Post Fusion AC

0-9s, Manual

Pulse Interval

0.1-9.9s (0.1sec resolution)

Shunt Resistor

Greater than 50Ω

Number of Pulses

1-99 (Automatic/Manual)

Power

100-115VAC, 220-240VAC

Dimensions, Weight

370W x 430D x 160H mm, 12.7kg







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