NEPA21 Cell Transfection Data
NEPA21 Cell Transfection Data Listing
V: Viability
TE: Transfection Efficiency
Cell Lines: With Electroporation Cuvettes
Cell Line |
Description, Species |
Characteristics, etc. |
Viability |
Efficiency |
Photo |
HeLa |
Human Cervical Carcinoma cells |
Epithelial, Immortalized |
95% |
95% |
Yes |
HeLa-K | Human Cervical Carcinoma cells | Epithelial, Immortalized Adherent |
90% | 90% | |
293 (HEK293) |
Human Embryonic Kidney cells |
Epithelial , Adherent |
90% |
90% |
|
293 (HEK293) |
Human Embryonic Kidney cells | Epithelial , Adherent | 90% | 70% | Yes |
293T (HEK293T) |
Human Embryonic Kidney cells |
Epithelial , Adherent |
90% |
90% |
Yes |
TIG-3 | Human Embryonic Lung Fibroblasts | Diploid, Normal Adherent |
90% | 80% | Yes |
TIG-7 |
Human Embryonic Lung Fibroblasts |
Diploid, Normal |
89% |
76% |
Yes |
MRC-5 | Human Embryonic Lung Fibroblasts | Diploid, Normal Adherent |
85% | 90% | Yes |
SUSM-1 | Human Fibroblasts | Fetal Liver, Epithelial, Normal, Immortalized | 77% (FACS) |
71% (FACS) |
Yes |
KMST-6 | Human Fibroblasts | Whole Embryo, Epithelial Normal, Immortalized |
70% | 60% | Yes |
hTERT-BJ1 | Human Fibroblast | Normal, Immortalized, Adherent | Good | 31% (FACS) |
|
HUVEC | Human Umbilical Vein Endothelial cells | Normal, Adherent | 70% | 75% | |
HT1080 | Human Fibrosarcoma cells | Connective Tissue Acetabulum, Adherent |
93% | 81% | Yes |
MIA-PaCa-2 | Human Pancreatic Carcinoma cells | Epithelial , Adherent | 80% | 77% | Yes |
HepG2 | Human Hepatoma cells | Epithelial , Adherent | 80% | 76% | Yes |
HuH-7 | Human Hepatoma cells | Epithelial , Adherent | 70% | 60% | Yes |
H69 | Human Small-Cell Lung Cancer cells | Adherent | 90% | 85% | Yes |
H1299 (NCI-H1299) |
Human Lung Cancer cells | Non-Small, Epithelial Adherent |
90% | 90% | |
HSC-2 | Human Squamous Carcinoma cells | Oral , Adherent | 90-95% | 98% | Yes |
HSC-3 | Human Squamous Carcinoma cells | Tongue , Adherent | 90-95% | 98% | Yes |
HSC-4 | Human Squamous Carcinoma cells | Tongue, Adherent | 80% | 34% | Yes |
Ca9-22 | Human Squamous Carcinoma cells | Gingival, Adherent | 60% | 60% | Yes |
HGF | Human Gingival Fibroblasts | Normal, Adherent | Excellent | Excellent | Yes |
MCF-7 | Human Breast Cancer cells | Epithelial, Adherent | 90% | 90-95% | |
T47D | Human Breast Cancer cells | Ductal Carcinoma Epithelial, Adherent |
90% | 80-90% | |
BT-20 | Human Breast Cancer cells | Adherent | 70% | 80% | Yes |
MCF-10A | Human Brest cells | Normal, Mammary Epithelial Adherent |
Excellent | Excellent | Yes |
NUGC-3 | Human Gastric Carcinoma cells | Adherent | 73% (FACS) |
68% (FACS) |
|
A549 | Human Lung Adenocarcinoma cells | Epithelial, Adherent | 80-90% | 90% | |
TE-1 | Human Esophageal Carcinoma cells | Adherent | 80-90% | 41% | Yes |
TE-8 | Human Esophageal Carcinoma cells | Adherent | 80-90% | 40% | Yes |
LNCaP | Human Prostate Carcinoma | Epithelial, Adherent | 71% (FACS) |
90% (FACS) |
|
PC-3 | Human Prostate Cancer cells | Epithelial, Adherent | 90% | 95% | |
RKO | Human Colorectal Carcinoma cells | Adherent | 80% | 40% | |
SKOV-3 | Human Ovarian Carcinoma cells | Adherent | 90% | 90% | Yes |
OVCAR-3 | Human Ovarian Carcinoma cells | Adherent | 90% | 79% | Yes |
RMG-1 | Human Ovarian Clear Cell Adenocarcinoma |
Adherent | 97% | 67% | Yes |
CCS | Clear Cell Sarcoma | Adherent | 47% | 55% | |
SK-N-SH | Human Neuroblastoma cells | Epithelial, Adherent | 95% | 95% | Yes |
SH-SY5Y | Human Neuroblastoma cells | Adherent | 60% | 90% | Yes |
KG-1-C | Human Oligodendroglial cells | Adherent | 85% | 60% | Yes |
A172 | Human Glioblastoma cells | Adherent | 80-90% | 70% | Yes |
1321N1 | Human Astrocytoma Cells | Adherent | 80% | 80% | Yes |
HaCaT | Human Keratinocyte cells | Normal, Immortalized Adherent |
40% | 80% | Yes |
CCD1079sk | Human Skin Fibroblasts | Adherent | 40% | 53% (FACS) |
Yes |
iHAM-4 | Human Amniotic Mesenchymal cells | Adherent | 59% | 95% | |
iHAE-7 | Human Amniotic Epithelial cells | Adherent | 70% | 40% | |
HTR-8/Svneo | Human Trophoblast cells | Adherent | 95% | 67% | Yes |
RPE | Retinal Pigment Epithelium cells | Adherent | 90% | 70% | |
RPTEC | Renal Proximal Tubule Epithelial cells | Adherent | 70% | 85% | |
UT-7/TPO | Human Megakaryocytic cells | Suspension | 51% | 46% | Yes |
Jurkat | Human T-cell Leukemia cells | Round Single, Blood, Suspension | 90% | 85% | Yes |
Nalm-6 | Human B-cell Precursor Leukemia cells | Blood, Suspension | 67% (FACS) |
70% (FACS) |
|
MY | Human Acute Lymphocytic Leukemia cells | Myelomonocytic, Suspension | 94% (FACS) |
22% (FACS) |
|
PALL-2 | Human Acute Leukemia cells | Acute Lymphoblastic | 60% | 27% (FACS) |
|
KG-1 | Human Acute Myeloid Leukemia cells | Suspension | 50% | 50% | Yes |
USCD/AML1 | Human Leukema cells | Suspension | 50% | 50% | |
Namalwa | Human Burkitt’s Lymphoma cells | B lymphocyte, Blood Suspension |
70% | 75% | |
Raji | Human Burkitt’s Lymphoma cells | B lymphocyte, Blood Suspension |
74% (FACS) |
53% (FACS) |
|
Mutu I | Human Burkitt Lymphoma cells | EBV Positive, Suspension | 87% (FACS) |
91% (FACS) |
Yes |
Mutu III | Human Burkitt Lymphoma cells | EBV Positive, Suspension | 54% (FACS) |
92% (FACS) |
|
HL-60 | Human Promyelocytic Leukemia cells | Suspension | 80% | 80% | |
LCL | Human Burkitt’s Lymphoma cells | Immortalized, Suspension | 55% (FACS) |
43% (FACS) |
Yes |
Ramos-BlueTM | Human Basophilic Leukemia cells | Suspension | 80% | 55% | Yes |
KU812 | Human Basophilic Leukemia cells | Suspension | 96% (FACS) |
42% (FACS) |
|
K562 | Human Chronic Myelogenous Leukemia cells | Suspension | 91% (FACS) |
99% (FACS) |
Yes |
THP-1 | Human Acute Moncytic Leukemia cells | Suspension | 76% | 63% | Yes |
NIH/3T3 | Mouse Embryonic Fibroblasts | Swiss NIH , Embryo Adherent |
74% (FACS) |
82% (FACS) |
Yes |
3T3-L1 | Mouse Preadipocyte | Fibroblast like | 90% | 90% | Yes |
MEF | Mouse Embryonic Fibroblasts | Adherent | 80% | 90% | |
MEF | Mouse Embryonic Fibroblasts | Adherent | 75% | 70% | Yes |
P19C6 | Mouse Embryonic Carcinoma Cells | Adherent | 90% | 50% | Yes |
L | Mouse Fibroblasts | Adherent | 90% | 60-70% | Yes |
MC3T3-E1 | Mouse Osteoblastic cells | Fibroblastic, Adherent | 85% | 75% | Yes |
LLc1 (LL/2) |
Lewis Lung Cell Carcinoma 1 cells | Adherent | 87% (FACS) |
81% (FACS) |
Yes |
C2C12 | Mouse Myoblast cells | Adherent | 80% | 70% | Yes |
4T1 | Mouse Breast Cancer cells | Adherent | 90% | 95% | Yes |
Colon-26 | Mouse Colon Adenocarcinoma cells | Adherent | 95% | 90% | Yes |
MS-1 | Mouse Pancreatic Endothelial cells | Adherent | 90% | 90% | |
ddy Mouse Endometrial cells | Fibroblast, Adherent | 60% | 80% | ||
Neuro-2a | Mouse Neuroblastoma Cells | Adherent | 90% | 90% | Yes |
BV-2 | Mouse Microglial cells | Adherent | 92% | 60% | Yes |
WR19L | Mouse T-cell Lymphoma cells | Suspension | 92% (FACS) |
60% (FACS) |
Yes |
BA/F3 | Mouse pro-B cells | Suspension | 90% | 90% | |
RAW264.7 | Mouse Macrophage-like cells | Monocyte-like, Adherent | 70% | 56% | Yes |
MIN6 | Mouse Pancreatic Beta cells | Suspension | 57% (FACS) |
71% (FACS) |
Yes |
Mouse Pancreatic Islet Beta cells | 60% | 40% | Yes | ||
MEL | Mouse Erythroleukemia cells | Suspension | 70% (FACS) |
50% (FACS) |
|
XS106 | Mouse Dendritic Cell | Suspension | 61% | 45% | Yes |
mDC | Mouse Myeloid Dendritic cells | Suspension | 79% (FACS) |
72% (FACS) |
|
MC/9 | Mouse Mast cells | Suspension | 76% (FACS) |
84% (FACS) |
Yes |
BMBa | Mouse Bone Marrow-Derived Basophils | Suspension | 45% (FACS) |
67% (FACS) |
Yes |
ATDC5 | Mouse Pre-chondrocytic cells | 62% | 14% | Yes | |
PC12 | Rat Adrenal Pheochromocytoma cells | Adherent | 90% | 70% | Yes |
H9c2 | Rat Ventricular Myoblasts | Skeletal Muscle-like Adherent |
71% (FACS) |
82% (FACS) |
Yes |
REF | Rat Embryonic Fibroblasts | Adherent | 90% | 99% | Yes |
RDF | Rat Dermal Fibroblasts | Adherent | 90% | 40% | |
C6 | Rat Glioma cells | Fibroblast-like, GFAP Positive, Adherent | 80% | 67% | Yes |
RSC96 | Rat Schwann cells | Adherent | 70% | 85% | Yes |
TtT/GF | Rat Anterior Pituitary cells | Folliculostellate, Adherent | 65% | 83% | Yes |
UMR106 | Rat Osteoblastic cells | Adherent | 80% | 70% | |
CHO | Chinese Hamster Ovary cells | Epithelial, Adherent | 74% | 90% | Yes |
CHO-K1 | Chinese Hamster Ovary cells | Epithelial, Adherent | 95% | 95% | Yes |
CHO-S | Chinese Hamster Ovary cells | Epithelial, Suspension | Excellent | 56% (FACS) |
|
DT40 | Chicken B cells | Suspension | 38% | 40% (FACS) |
Yes |
MDCK | Madrin-Darby Canine Kidney cells | Epithelial-like, Adherent | 90% | 95% | Yes |
BFF | Bovine Fetal Fibroblasts | Adherent | 78% (FACS) |
72% (FACS) |
Yes |
A6 | Xenopus Kidney Epithelial cells | Adherent | 90% | 60% | Yes |
GTS9 | Ginbuna Thymus cells | Adherent | 50% | 30% | Yes |
Vero | African Green Monkey Kidney cells | Epithelial-like, Adherent | 58% | 35% | |
SEC | Bovine T cells | Suspension | 80% | 21% (FACS) |
.
Primary Cells: With Electroporation Cuvettes
Cell Line | Description, Species | Characteristics, etc. | Viability | Efficiency | Photo |
HASM | Human Airway Smooth Muscle cells | Primary, Adherent | 90% | 80% | Yes |
Human Endometrial Stromal cells | Primary Adherent | 95% | 90% | Yes | |
Human Uterine Cervical Fibroblast | Primary, Adherent | 65% | 90% | Yes | |
Human Amniotic Mesenchymal cells | Primary, Adherent | 70% | 40% | ||
Human T cells | Primary, Suspension | 58% (FACS) |
90% (FACS) |
Yes | |
Mouse Cerebral Cortex Neurons (Embryonic day14) |
Primary, Adherent | 80% | 70% | Yes | |
Mouse Hippocampal Neurons (Embryonic day 14) |
Primary, Adherent | 80% | 60% | ||
Mouse Basal Ganglia Primordium (Embryonic day 13.5) |
Primary, Adherent | 91% (FACS) |
71% (FACS) |
||
Mouse Microglia cells | Primary, Adherent | 60-70% | 23% | Yes | |
MEF | Mouse Embryonic Fibroblasts | Primary, Adherent | 90% | 85% | Yes |
Mouse Embryonic Skin Fibroblasts | Primary, Adherent | 90% | 50% | Yes | |
BMMC (BMMCs) |
Mouse Bone Marrow-Derived Mast cells | Primary, Suspension | 64% (FACS) |
78% (FACS) |
Yes |
Mouse Primary Liver cells | Primary, Adherent | 70% | 50% | Yes | |
Mouse Stromal cells | Primary, Adherent | 90% | 30% | ||
Rat Cerebral Cortex Neurons |
Primary, Adherent | 64% | 78% | Yes | |
Rat Hippocampal Neurons | Primary, Adherent | 60% | 80% | ||
Rat Bulbar Neurons (Embryonic day 15) |
Primary, Adherent | 80% | 75% | Yes | |
Rat Cerebellar Granule cells | Primary, Adherent | 70% | 80% | ||
Rat Meningeal Fibroblasts (Postnatal day 3) |
Primary, Adherent | 90% | 95% | Yes | |
OEC | Rat Olfactory Ensheathing cells (Postnatal week 3) |
Primary, Adherent | 93% | 46% | Yes |
Chick Embryonic Fibroblasts | Primary Adherent | 80% | 90% | Yes | |
Chick Embryonic Cerebellar Granule cells (Embryonic day 11) |
Primary, Adherent | 86% | 83% | Yes |
.
Stem Cells, ES Cells and iPS Cells: With Electroporation Cuvettes
Cell Line | Description, Species | Characteristics, etc. | Viability | Efficiency | Photo |
Human Mesenchymal stem cells | Primary, Adherent Cord blood derived |
78% (FACS) |
75% (FACS) |
Yes | |
TS | Mouse Trophoblast Stem cells | Adherent | 59% | 47% | Yes |
TT2 | Mouse TT2 ES cells | Adherent | 50-60% | 50-60% | Yes |
Mouse ES cells | Adherent | 80% | 75% | Yes | |
Rat WDA ES-like cells | Adherent | 60% | 80% | ||
Mouse iPS Cells | Origin: Mouse Fibroblast, Adherent | 89% | 47% | Yes | |
Mouse Neurospheres Neural Stem cells Derrived from Mouse SVZ |
Excellent | Excellent | Yes | ||
Mouse Neural Stem cells | Primary, Adherent | Good | 37% | Yes |
.
Cells Lines in Adherence: With Adherent-Cell Electrodes
Cell Line | Description, Species | Characteristics, etc. | Viability | Efficiency | Photo |
SH-SY5Y | Human Neuroblastoma cells | Adherent | 90% | 50% | Yes |
EPC | Human Endothelia Progenitor cells | Adherent | Yes | ||
3T3-L1 | Mouse Preadipocytes | Adherent | 90% | 70% | Yes |
C6 | Rat Glioma cells | Fibroblast-like, GFAP Positive, Adherent | 80% | 39% | Yes |
.
Primary Cell in Adherence: With Adherent-Cell Electrodes
Cell | Characteristics | Viability | Efficiency | Photo | |
Human Epidermal Keratinocyte cell | Primary, Adherent | 90% | 30% | ||
Mouse Cerebral Cortex Neurons (Embryonic day 15) (6 DIV) |
Primary, Adherent | Excellent | Excellent | Yes | |
Mouse Hippocampal Neurons (Embryonic day 14) (4 DIV) |
Primary, Adherent | 60% | 50% | ||
Mouse Hippocampal Neurons (Embryonic day 18) (2 DIV) |
Primary, Adherent | 85% | 54% | Yes | |
Mouse Stromal cells (1-month cultured) |
Primary, Adherent | 90% | 50% | ||
Mouse Glial cells (14 DIV) |
Primary, Adherent | 80% | 50% | Yes | |
Mouse Cerebral Cortex Neurons (Embryonic day 17) (2 DIV) |
Primary, Adherent | 70% | 60% | Yes | |
Rat Granulosa cells | Primary, Adherent | Excellent | 41% | ||
Rat Neurons (E17 rat cerebral cortex. After 2 DIV. on 24-well plates) |
Primary, Adherent | 70% | 60% | Yes | |
Rat Neurons (P7 rat hippocampus. After 11 DIV) |
Primary, Adherent | 100% | 50% | Yes |
.
Plant Protoplasts
Cell | Characteristics | Luciferase Activity (CPS) |
||
Tabacco Protoplasts | 20,312 |