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NEPA21 Electroporator
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Product Code: OCH01

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Applic:Electroporation and Sonoporation


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Product Code: CUY562-0.5

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0.4mm x 0.4mm platinum plate electrode & replaceable needle electrodes, 0.5mm gap

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NEPA21 Cell Transfection Data

NEPA21 Cell Transfection Data Listing

V:  Viability
TE: Transfection Efficiency

Cell Lines: With Electroporation Cuvettes

Cell Line
Description, Species

Characteristics, etc.

Viability

Efficiency

Photo
HeLa

Human Cervical Carcinoma cells

Epithelial, Immortalized
Adherent

95%

95%

Yes
HeLa-K Human Cervical Carcinoma cells Epithelial, Immortalized
Adherent
90% 90%
293
(HEK293)

Human Embryonic Kidney cells

Epithelial , Adherent
Adenovirus type 5

90%

90%

293
(HEK293)
Human Embryonic Kidney cells Epithelial , Adherent 90% 70% Yes
293T
(HEK293T)

Human Embryonic Kidney cells

Epithelial , Adherent
SV40 large T antigen

90%

90%

Yes
TIG-3 Human Embryonic Lung Fibroblasts Diploid, Normal
Adherent
90% 80% Yes
TIG-7

Human Embryonic Lung Fibroblasts

Diploid, Normal
Adherent

89%
(FACS)

76%
(FACS)

Yes
MRC-5 Human Embryonic Lung Fibroblasts Diploid, Normal
Adherent
85% 90% Yes
SUSM-1 Human Fibroblasts Fetal Liver, Epithelial, Normal, Immortalized 77%
(FACS)
71%
(FACS)
Yes
KMST-6 Human Fibroblasts Whole Embryo, Epithelial
Normal, Immortalized
70% 60% Yes
hTERT-BJ1 Human Fibroblast Normal, Immortalized, Adherent Good 31%
(FACS)
HUVEC Human Umbilical Vein Endothelial cells Normal, Adherent 70% 75%
HT1080 Human Fibrosarcoma cells Connective Tissue
Acetabulum, Adherent
93% 81% Yes
MIA-PaCa-2 Human Pancreatic Carcinoma cells Epithelial , Adherent 80% 77% Yes
HepG2 Human Hepatoma cells Epithelial , Adherent 80% 76% Yes
HuH-7 Human Hepatoma cells Epithelial , Adherent 70% 60% Yes
H69 Human Small-Cell Lung Cancer cells Adherent 90% 85% Yes
H1299
(NCI-H1299)
Human Lung Cancer cells Non-Small, Epithelial
Adherent
90% 90%
HSC-2 Human Squamous Carcinoma cells Oral , Adherent 90-95% 98% Yes
HSC-3 Human Squamous Carcinoma cells Tongue , Adherent 90-95% 98% Yes
HSC-4 Human Squamous Carcinoma cells Tongue, Adherent 80% 34% Yes
Ca9-22 Human Squamous Carcinoma cells Gingival, Adherent 60% 60% Yes
HGF Human Gingival Fibroblasts Normal, Adherent Excellent Excellent Yes
MCF-7 Human Breast Cancer cells Epithelial, Adherent 90% 90-95%
T47D Human Breast Cancer cells Ductal Carcinoma
Epithelial, Adherent
90% 80-90%
BT-20 Human Breast Cancer cells Adherent 70% 80% Yes
MCF-10A Human Brest cells Normal, Mammary Epithelial
Adherent
Excellent Excellent Yes
NUGC-3 Human Gastric Carcinoma cells Adherent 73%
(FACS)
68%
(FACS)
A549 Human Lung Adenocarcinoma cells Epithelial, Adherent 80-90% 90%
TE-1 Human Esophageal Carcinoma cells Adherent 80-90% 41% Yes
TE-8 Human Esophageal Carcinoma cells Adherent 80-90% 40% Yes
LNCaP Human Prostate Carcinoma Epithelial, Adherent 71%
(FACS)
90%
(FACS)
PC-3 Human Prostate Cancer cells Epithelial, Adherent 90% 95%
RKO Human Colorectal Carcinoma cells Adherent 80% 40%
SKOV-3 Human Ovarian Carcinoma cells Adherent 90% 90% Yes
OVCAR-3 Human Ovarian Carcinoma cells Adherent 90% 79% Yes
RMG-1 Human Ovarian Clear Cell
Adenocarcinoma
Adherent 97% 67% Yes
CCS Clear Cell Sarcoma Adherent 47% 55%
SK-N-SH Human Neuroblastoma cells Epithelial, Adherent 95% 95% Yes
SH-SY5Y Human Neuroblastoma cells Adherent 60% 90% Yes
KG-1-C Human Oligodendroglial cells Adherent 85% 60% Yes
A172 Human Glioblastoma cells Adherent 80-90% 70% Yes
1321N1 Human Astrocytoma Cells Adherent 80% 80% Yes
HaCaT Human Keratinocyte cells Normal, Immortalized
Adherent
40% 80% Yes
CCD1079sk Human Skin Fibroblasts Adherent 40% 53%
(FACS)
Yes
iHAM-4 Human Amniotic Mesenchymal cells Adherent 59% 95%
iHAE-7 Human Amniotic Epithelial cells Adherent 70% 40%
HTR-8/Svneo Human Trophoblast cells Adherent 95% 67% Yes
RPE Retinal Pigment Epithelium cells Adherent 90% 70%
RPTEC Renal Proximal Tubule Epithelial cells Adherent 70% 85%
UT-7/TPO Human Megakaryocytic cells Suspension 51% 46% Yes
Jurkat Human T-cell Leukemia cells Round Single, Blood, Suspension 90% 85% Yes
Nalm-6 Human B-cell Precursor Leukemia cells Blood, Suspension 67%
(FACS)
70%
(FACS)
MY Human Acute Lymphocytic Leukemia cells Myelomonocytic, Suspension 94%
(FACS)
22%
(FACS)
PALL-2 Human Acute Leukemia cells Acute Lymphoblastic 60% 27%
(FACS)
KG-1 Human Acute Myeloid Leukemia cells Suspension 50% 50% Yes
USCD/AML1 Human Leukema cells Suspension 50% 50%
Namalwa Human Burkitt’s Lymphoma cells B lymphocyte, Blood
Suspension
70% 75%
Raji Human Burkitt’s Lymphoma cells B lymphocyte, Blood
Suspension
74%
(FACS)
53%
(FACS)
Mutu I Human Burkitt Lymphoma cells EBV Positive, Suspension 87%
(FACS)
91%
(FACS)
Yes
Mutu III Human Burkitt Lymphoma cells EBV Positive, Suspension 54%
(FACS)
92%
(FACS)
HL-60 Human Promyelocytic Leukemia cells Suspension 80% 80%
LCL Human Burkitt’s Lymphoma cells Immortalized, Suspension 55%
(FACS)
43%
(FACS)
Yes
Ramos-BlueTM Human Basophilic Leukemia cells Suspension 80% 55% Yes
KU812 Human Basophilic Leukemia cells Suspension 96%
(FACS)
42%
(FACS)
K562 Human Chronic Myelogenous Leukemia cells Suspension 91%
(FACS)
99%
(FACS)
Yes
THP-1 Human Acute Moncytic Leukemia cells Suspension 76% 63% Yes
NIH/3T3 Mouse Embryonic Fibroblasts Swiss NIH , Embryo
Adherent
74%
(FACS)
82%
(FACS)
Yes
3T3-L1 Mouse Preadipocyte Fibroblast like 90% 90% Yes
MEF Mouse Embryonic Fibroblasts Adherent 80% 90%
MEF Mouse Embryonic Fibroblasts Adherent 75% 70% Yes
P19C6 Mouse Embryonic Carcinoma Cells Adherent 90% 50% Yes
L Mouse Fibroblasts Adherent 90% 60-70% Yes
MC3T3-E1 Mouse Osteoblastic cells Fibroblastic, Adherent 85% 75% Yes
LLc1
(LL/2)
Lewis Lung Cell Carcinoma 1 cells Adherent 87%
(FACS)
81%
(FACS)
Yes
C2C12 Mouse Myoblast cells Adherent 80% 70% Yes
4T1 Mouse Breast Cancer cells Adherent 90% 95% Yes
Colon-26 Mouse Colon Adenocarcinoma cells Adherent 95% 90% Yes
MS-1 Mouse Pancreatic Endothelial cells Adherent 90% 90%
ddy Mouse Endometrial cells Fibroblast, Adherent 60% 80%
Neuro-2a Mouse Neuroblastoma Cells Adherent 90% 90% Yes
BV-2 Mouse Microglial cells Adherent 92% 60% Yes
WR19L Mouse T-cell Lymphoma cells Suspension 92%
(FACS)
60%
(FACS)
Yes
BA/F3 Mouse pro-B cells Suspension 90% 90%
RAW264.7 Mouse Macrophage-like cells Monocyte-like, Adherent 70% 56% Yes
MIN6 Mouse Pancreatic Beta cells Suspension 57%
(FACS)
71%
(FACS)
Yes
Mouse Pancreatic Islet Beta cells 60% 40% Yes
MEL Mouse Erythroleukemia cells Suspension 70%
(FACS)
50%
(FACS)
XS106 Mouse Dendritic Cell Suspension 61% 45% Yes
mDC Mouse Myeloid Dendritic cells Suspension 79%
(FACS)
72%
(FACS)
MC/9 Mouse Mast cells Suspension 76%
(FACS)
84%
(FACS)
Yes
BMBa Mouse Bone Marrow-Derived Basophils Suspension 45%
(FACS)
67%
(FACS)
Yes
ATDC5 Mouse Pre-chondrocytic cells 62% 14% Yes

PC12 Rat Adrenal Pheochromocytoma cells Adherent 90% 70% Yes
H9c2 Rat Ventricular Myoblasts Skeletal Muscle-like
Adherent
71%
(FACS)
82%
(FACS)
Yes
REF Rat Embryonic Fibroblasts Adherent 90% 99% Yes
RDF Rat Dermal Fibroblasts Adherent 90% 40%
C6 Rat Glioma cells Fibroblast-like, GFAP Positive, Adherent 80% 67% Yes
RSC96 Rat Schwann cells Adherent 70% 85% Yes
TtT/GF Rat Anterior Pituitary cells Folliculostellate, Adherent 65% 83% Yes
UMR106 Rat Osteoblastic cells Adherent 80% 70%
CHO Chinese Hamster Ovary cells Epithelial, Adherent 74% 90% Yes
CHO-K1 Chinese Hamster Ovary cells Epithelial, Adherent 95% 95% Yes
CHO-S Chinese Hamster Ovary cells Epithelial, Suspension Excellent 56%
(FACS)
DT40 Chicken B cells Suspension 38% 40%
(FACS)
Yes
MDCK Madrin-Darby Canine Kidney cells Epithelial-like, Adherent 90% 95% Yes
BFF Bovine Fetal Fibroblasts Adherent 78%
(FACS)
72%
(FACS)
Yes
A6 Xenopus Kidney Epithelial cells Adherent 90% 60% Yes
GTS9 Ginbuna Thymus cells Adherent 50% 30% Yes
Vero African Green Monkey Kidney cells Epithelial-like, Adherent 58% 35%
SEC Bovine T cells Suspension 80% 21%
(FACS)

.

Primary Cells: With Electroporation Cuvettes

Cell Line Description, Species Characteristics, etc. Viability Efficiency Photo
HASM Human Airway Smooth Muscle cells Primary, Adherent 90% 80% Yes
Human Endometrial Stromal cells Primary Adherent 95% 90% Yes
Human Uterine Cervical Fibroblast Primary, Adherent 65% 90% Yes
Human Amniotic Mesenchymal cells Primary, Adherent 70% 40%
Human T cells Primary, Suspension 58%
(FACS)
90%
(FACS)
Yes
Mouse Cerebral Cortex Neurons
(Embryonic day14)
Primary, Adherent 80% 70% Yes
Mouse Hippocampal Neurons
(Embryonic day 14)
Primary, Adherent 80% 60%
Mouse Basal Ganglia Primordium
(Embryonic day 13.5)
Primary, Adherent 91%
(FACS)
71%
(FACS)
Mouse Microglia cells Primary, Adherent 60-70% 23% Yes
MEF Mouse Embryonic Fibroblasts Primary, Adherent 90% 85% Yes
Mouse Embryonic Skin Fibroblasts Primary, Adherent 90% 50% Yes
BMMC
(BMMCs)
Mouse Bone Marrow-Derived Mast cells Primary, Suspension 64%
(FACS)
78%
(FACS)
Yes
Mouse Primary Liver cells Primary, Adherent 70% 50% Yes
Mouse Stromal cells Primary, Adherent 90% 30%

Rat Cerebral Cortex Neurons
(Embryonic day 16)

Primary, Adherent 64% 78% Yes
Rat Hippocampal Neurons Primary, Adherent 60% 80%
Rat Bulbar Neurons
(Embryonic day 15)
Primary, Adherent 80% 75% Yes
Rat Cerebellar Granule cells Primary, Adherent 70% 80%
Rat Meningeal Fibroblasts
(Postnatal day 3)
Primary, Adherent 90% 95% Yes
OEC Rat Olfactory Ensheathing cells
(Postnatal week 3)
Primary, Adherent 93% 46% Yes
Chick Embryonic Fibroblasts Primary Adherent 80% 90% Yes
Chick Embryonic Cerebellar Granule cells
(Embryonic day 11)
Primary, Adherent 86% 83% Yes

.

Stem Cells, ES Cells and iPS Cells: With Electroporation Cuvettes

Cell Line Description, Species Characteristics, etc. Viability Efficiency Photo
Human Mesenchymal stem cells Primary, Adherent
Cord blood derived
78%
(FACS)
75%
(FACS)
Yes
TS Mouse Trophoblast Stem cells Adherent 59% 47% Yes
TT2 Mouse TT2 ES cells Adherent 50-60% 50-60% Yes
Mouse ES cells Adherent 80% 75% Yes
Rat WDA ES-like cells Adherent 60% 80%
Mouse iPS Cells Origin: Mouse Fibroblast, Adherent 89% 47% Yes
Mouse Neurospheres
Neural Stem cells Derrived from Mouse SVZ
Excellent Excellent Yes
Mouse Neural Stem cells Primary, Adherent Good 37% Yes

.

Cells Lines in Adherence: With Adherent-Cell Electrodes

Cell Line Description, Species Characteristics, etc. Viability Efficiency Photo
SH-SY5Y Human Neuroblastoma cells Adherent 90% 50% Yes
EPC Human Endothelia Progenitor cells Adherent Yes
3T3-L1 Mouse Preadipocytes Adherent 90% 70% Yes
C6 Rat Glioma cells Fibroblast-like, GFAP Positive, Adherent 80% 39% Yes

.

Primary Cell in Adherence: With Adherent-Cell Electrodes

Cell Characteristics Viability Efficiency Photo
Human Epidermal Keratinocyte cell Primary, Adherent 90% 30%
Mouse Cerebral Cortex Neurons
(Embryonic day 15) (6 DIV)
Primary, Adherent Excellent Excellent Yes
Mouse Hippocampal Neurons
(Embryonic day 14)  (4 DIV)
Primary, Adherent 60% 50%
Mouse Hippocampal Neurons
(Embryonic day 18)  (2 DIV)
Primary, Adherent 85% 54% Yes
Mouse Stromal cells
(1-month cultured)
Primary, Adherent 90% 50%
Mouse Glial cells
(14 DIV)
Primary, Adherent 80% 50% Yes
Mouse Cerebral Cortex Neurons
(Embryonic day 17) (2 DIV)
Primary, Adherent 70% 60% Yes
Rat Granulosa cells Primary, Adherent Excellent 41%
Rat Neurons
(E17 rat cerebral cortex. After 2 DIV. on 24-well plates)
Primary, Adherent 70% 60% Yes
Rat Neurons
(P7 rat hippocampus. After 11 DIV)
Primary, Adherent 100% 50% Yes

.

Plant Protoplasts

Cell Characteristics Luciferase Activity
(CPS)
Tabacco Protoplasts 20,312


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