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NEPA21 Electroporator
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Transgenic Mouse Zygote Electroporation: NEPA21
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Featured Device

2
Product Code: SONIDEL MB101

Desc:Microbubble for Ultrasound-mediated Transfection
Applic:Sonoporation Aid


Featured Electrode



Product Code: CUY5100-100

Application:
Electrodes for Electro Cell Fusion
Description:
Chopstick Stainless Steel, needle electrode, 100Ķmφ, 5mm tip length, 2/pkg

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Mouse/Rat – Skin

Electrode Recommendation:

To deliver genes into Mouse/Rat Skin by electroporation, we recommend:

–¬†¬†¬†¬†¬†¬†¬†¬†¬† CUY663-5×10 electrode

This electrode is a tweezers-type electrode.  One tweezers is a 5 x 10mm rectangular electrode and the other is three needles which look like a fork.

For your further information, some clients prefer to use the CUY650P electrode series for the same application.  The CUY650P series is also a tweezers type electrode but it incorporates disk electrodes.  With the CUY650P series, skin is picked up and simply sandwiched between the disk electrodes.

Technical Drawing: CUY650 series

 

Protocol Recommendation:

Kindly note Dr. Maruyama’s excellent know-how article:

–¬† Epidermis-targeted gene transfer using in vivo electroporation

In this article Dr. Maruyama uses the CUY21EDIT electroporator and CUY663B (currently catalogue number CUY663-5×10) electrode.

According to Dr. Maruyama’s paper, the required voltage is maximum 24V. ¬†The appropriate electrode diameter depends on the size of the area into which the genes are to be transfected.¬† If one’s target area is small, the 1mm diameter (CUY650P1) electrode is appropriate.

Also of know are the following articles:

Р Chapter 27: Electroporation-Mediated Intradermal Delivery of DNA Vaccines in Nonhuman Primates

Modulation of scratching behavior by silencing an endogenous cyclooxygenase-1 gene in the skin through the administration of siRNA

 

Click here to view a summary of the unique advantages of the NEPA21 electroporation system and its novel multi-pulse EP Methodology


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