Adult Mouse and Rat (In vivo) – Liver
In Vivo and Ex Vivo – Rat and Mouse
In Vivo Liver EP
For Adult Mouse and Rat
Please see below for a full list of relevant NEPA21 publications.
For the best methodology and set-up preparation, we recommend the paper:
“Adjuvant gemcitabine therapy improves survival in a locally induced, R0-resectable model of metastatic intrahepatic cholangiocarcinoma”
The electrode we recommend is our CUY650P5 electrode.
You will note that the EP Conditions recommended in this paper are single-pulse parameters (as the researchers used our old CUY21SC system).
Our NEPA21 parameters are multipulse. If you have the NEPA21 system, please contact us (firstname.lastname@example.org) for a set of EP parameters. If you do not have a NEPA21 system, please contact us for a demonstration.
While it is not necessary for successful electroporation, one might improve Transfection Efficiency by Clamping the lobes. We have clients who have clamped lobes and obtained good results.
However, clamping is not an easy procedure. But if the expertise is available in your lab., we recommend clamping.
Mouse and Rat – In Vivo Liver Publications, Protocols and electrode recommendations
|P on||P off||
No of Pulses
|Mouse/Rat in vivo Liver||Gürlevik E et al.Adjuvant gemcitabine therapy improves survival in a locally induced, R0-resectable model of metastatic intrahepatic cholangiocarcinoma.Hepatology. 2013 Sep;58(3):1031-41.||CUY650P5||Ask Us||Ask Us||Ask Us||Ask Us|
|Zang et al.Inhibition of nuclear delivery of plasmid DNA and transcription by interferon γ: hurdles to be overcome for sustained gene therapy.Gene Ther. 2011 Sep;18(9):891-7. doi: 10.1038/gt.2011.35. Epub 2011 Mar 31.||CUY650P10||Ask Us||Ask Us||Ask Us||Ask Us|
|Kiyama S et al.Reduction of fibrosis in a rat model of non-alcoholic steatohepatitis cirrhosis by human HGF gene transfection using electroporation.J Gastroenterol Hepatol. 2008 Aug;23(8 Pt 2):e471-6.||CUY650P10||Ask Us||Ask Us||Ask Us||Ask Us|
|Nakagawa et al. Deubiquitylation of histone H2A activates transcriptional initiation via trans-histone cross-talk with H3K4 di- and trimethylation Genes & Development, Volume 22, Issue 1, Pages 37-49, 1 January 2008||CUY650P series||Ask Us||Ask Us||Ask Us||Ask Us|
|Kawano T et al. Stabilizing of plasmid DNA in vivo by PEG-modified cationic gold nanoparticles and the gene expression assisted with electrical pulsesJ Control Release. 2006 Apr 10;111(3):382-9.||CUY650P5||Ask Us||Ask Us||Ask Us||Ask Us|
|Sakai et al. Hepatocyte-targeted gene transfer by combination of vascularly delivered plasmidDNA and in vivo electroporationGene Therapy, Volume 12, Issue 7, Pages 607-616, April 2005||CUY650P10||Ask Us||Ask Us||Ask Us||Ask Us|
|MouseLiver, Kidney,Spleen, Skin andMuscle||Thanaketpaisarn et al., Tissue-Specific Characteristics of in Vivo Electric Gene: Transfer by Tissue andIntravenous Injection of Plasmid DNAPharmaceutical Research, Volume 22, Number 6, Pages 883-891, June 2005||CUY650P5||Ask Us||Ask Us||Ask Us||Ask Us|
|Mice/Rats in vivo Muscle||Xue Hepatocyte growth factor gene therapy accelerates regeneration in cirrhotic mouse liver after hepatectormy Gut, Volume 52, Number 5, Pages 694-700, May 2003||CUY650-10||Ask Us||Ask Us||Ask Us||Ask Us|
|Xue et al. Attenuated acute liver injury in mice by naked hepatocyte growth factor gene transferinto skeletal muscle with electroporationGut, Volume 50, Number 4, Pages 558-562, April 2002||CUY650-10||Ask Us||Ask Us||Ask Us||Ask Us|
Postnatal (P0) EP – Mouse Retina Ex Vivo EP
We recommend the CUY650P7 electrode.
Please note the following;
– For Methodology: Efficient In Vivo Electroporation of the Postnatal Rodent Forebrain
Re Liver Cells
To review photographic results of experiments performed at client labs. and to appreciate the quantum of the results we guarantee. Please click on the following link to our website:
– Images of Cells Transfected (in Client labs.)
– List of Cells Transfected (in Client labs.)
For both links: After you open them:
– Hold down the ‘Control’ key and pressing the ‘F’ key
– This will open a search field
– To search for result data relevant to your interest, please search under a relevant search ‘term’:
– E.g., ‘liv’ (for Liver Cell data) ‘ips’ (for iPSC Cell data)
– Type the relevant search term in the in the search field and hit the return key
Re Ex Vivo Slice EP
There are a number of application options for Slice EP:
Cuvette EP Option
– with the CU500 Cuvette Holder and our EC-002S cuvettes
Ex Vivo electrode Options
– CUY520P5 (Link to CUY520P5 photo)
– CUY701PxE and CUY701PxL (Link to photo: CUY701pxE and CUY701PxL configurations )
– CUY700PxE and CUY700PxL (Link to photo: CUY700PxE and CUY700PxL configurations)
To review sample photographic results for Ex Vivo EP, please note the following links to our website:
– Ex Vivo Results
– Ex vivo and In Vivo (Retina)
– Mouse and Rat Brain Slice and Tissue Slice EP
– Mouse and Rat Small Tissue EP
– Cultured Mouse (Whole Embryo and Large Tissue EP
– Embryonic Tissues (Kidney and Lungs) and Small Tissues
– In Vivo Spinal Cord EP
– Mouse Embryo – Retina