Mouse and Rat Small Tissue Electrode Recommendation and Protocol
Mouse and Rat Small Tissue Electrode Recommendations and Protocols:
If you wish to perform electroporation with small tissue, the
- CUY700 series and
- CUY701 series
may also be used for this purpose.
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA. For such applications we believe that the electroporation methods described in ‘Electroporation-mediated gene transfer system applied to cultured CNS neurons’ are appropriate, that is, placing an electrode on the top of a tissue which is on another electrode. A number of electrode option recommendations are appropriate:
Electrode Option 1:
- CUY700P2E and CUY700P1L or CUY195P0.5
Technical Drawings: CUY700P2E CUY700P1L CUY195 series
Electrode Option 2:
- CUY700P2E and CUY614 w/CUY615, one CUY613P1 or CUY611P3-1
Technical Drawings:
Download the full papers described briefly in ‘Electroporation-mediated gene transfer system applied to cultured CNS neurons’ above.
- Dr. Okabe paper ‘Electroporation-mediated gene transfer system applied to cultured CNS neurons’, and
- Dr. Shimamura’s paper ‘Early subdivisions in the neural plate define distinct comptence for inductive signals’
Cultured Mouse Embryo and Large Tissue:
If you wish to transfect genes into a cultured mouse embryo we recommend the
- CUY520 series
Technical Drawing: CUY520 sries
The volume of a bath can be changed by placing a small bath made of agarose gel in the bath. Please refer to ‘Method to introduce genes into epithelial cells of the chicken embryonic stomach (proventriculus)‘.
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See also Mouse and Rat Tissue/Brain Slice Recommendations