Cultured Mouse Embryos and Large Tissue
Cultured Mouse Embryo and Large Tissue:
If you wish to transfect genes into a cultured mouse embryo we recommend the
- CUY520 series                                Technical Drawing: CUY520 series
The volume of a bath can be changed by placing a small bath made of agarose gel in the bath. Please refer to ‘Method to introduce genes into epithelial cells of the chicken embryonic stomach (proventriculus)‘.
Small Tissue:
If you wish to perform electroporation with small tissue, the
-Â Â Â Â Â Â Â Â Â Â CUY700 series and
-Â Â Â Â Â Â Â Â Â Â CUY701 series
may also be used for this purpose.
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA. For such applications we believe that the electroporation methods described in:
- ‘Electroporation-mediated gene transfer system applied to cultured CNS neurons’
- ‘Early subdivisions in the neural plate define distinct comptence for inductive signals’
are appropriate, that is, placing an electrode on the top of a tissue which is on another electrode. A number of electrode option recommendations are appropriate:
Electrode Option 1:
-Â Â Â Â Â Â Â Â Â Â CUY700P2E and CUY700P1L or CUY195P0.5
Technical Drawing: CUY700P2EÂ Â Â Â Â Â Â Â Â Â Â Technical Drawing: 700P1L
Electrode Option 2:
-Â Â Â Â Â Â Â Â Â CUY700P2E and CUY614 with CUY615, one CUY613P1 or CUY611P3-1
Technical Drawing: CUY700P2EÂ Â Â Â Â Â Technical Drawing: CUY614 and CUY615
Technical Drawing: CUY613P1Â Â Â Â Â Â Â Â Â Â Technical Drawing: CUY611P3-1