SONIDEL Limited      :: sales@sonidel.com        :: tech-support@sonidel.com         :: Tel:  IRE, ++353 (0)1 4434358 UK, ++44 (0)20 3239 4904 USA, ++1 (617) 297 2435 .
Most Popular

- New Products
- STK10: Ultrasound   Transfection Kit
- SP100: Sonoporator
- SDM-series: Microbubbles
- MB101: Microbubble
- NEPA21 Electroporator
- NEPA21: Illustrated   Applications
- NEPA21: Publications by   Research Application
- Connection Cables
- CU700: Monitoring System
- CU902: Polarity Exchanger
- CUY21 EDIT Electroporator
- CUY21 EDIT-S Electroporator
- CUY21 SC Electroporator
- In Vivo, In vitro Electrodes
- Electroporation Cuvettes
- Cuvette Chamber & Stand   Holder
- Laser Thermal Microinjector
- Mechanical Vibration Units
- Ultrasounic BioMicroscope
- KTAC-4000: Sonoporator
- LF101: Cell Fusion Device
- LF201: Cell Fusion Device
- Electro Cell Fusion Electrodes
Electroporation and Electro   Cell Fusion Accessories
- Electroporators
- Publications/Protocols
- Electrodes by Product Codes

 

Product Search
Search by product code, name or application:


Full Product List


Electrode Search
Search for the most appropriate electrode by research application:

Publications/Protocols
- Sonoporation
- Electroporation
- ElectroCell Fusion
- What Is Sonoporation?

Electrode Recommendation
- Electrode Recommendations

Featured Applications
Sonoporation
SONIDEL SP100 and KTAC4000
Electroporation
CUY21 EDIT, CUY21 SC
Electro Cell Fusion
LF101 and LF201

Newsletter Subscribe

Please enter your email:


Featured Device

0
Product Code: SONIDEL SP100

Desc:Sonoporator
Applic:(hover for application)Application:
  • Delivery of plasmids to cells and tissues for gene therapy-based applications and studies.
  • Delivery of nucleic acids such as siRNA, RNAi etc. to cells and tissues for studies on control of gene expression/gene therapy.
  • Delivery of cancer chemotherapeutic agents to impermeable target cells/tissues.
  • Delivery of agents to cells to study metabolic effects.


Featured Electrode



Product Code: CUY650P5

Application:
Electrodes for Electroporation
Description:
Tweezers w/5mmf platinum disk electrodes

Information
- How to order
- Contact us

Cultured Mouse Embryos and Large Tissue

Cultured Mouse and Rat Embryo and Large Tissue EP

Whole Mammalian Embryo EP:
There is a choice of two different electrodes:
-    CUY520P20 and
-    CUY650P7
(Click on links to view a technical drawing of the electrodes or similar electrodes in the series)

Please note the following article which explains the appropriate Methodology:
-    Electroporation into Cultured Mammalian Embryos
-    Methodology Summarised

Applicable Stages for Whole-Embryo Culture

E8.5

9.5

10.5

11.5

12.5

13.5

14.5

15.5

Rat Stages

6.5

7.5

8.5

9.5

10.5

11.5

12.5

13.5

Mouse Stages

NOTES
The volume of a bath can be changed by placing a small bath made of agarose gel in the bath.
Please refer to the Application Note: ‘Method to introduce genes into epithelial cells of the chicken embryonic stomach (proventriculus)’.

Embryonic Tissues (Kidney and Lung)
Result form client lab. for Embryonic Tissues (Kidney and Lung) using our CUY520P5 electrode (technical drawing).

 NEPA21 – Ex Vivo Publications
- Acute Inhibition of PI3K-PDK1-Akt Pathway Potentiates Insulin Secretion through Upregulation of Newcomer Granule Fusions in Pancreatic β-Cells
- Cortical excitatory neurons become protected from cell division during neurogenesis in an Rb family-dependent manner
- Electroporation-mediated gene transfer system applied to cultured CNS neurons
- Imaging of Insulin Exocytosis from Pancreatic Beta Cells
- In vivo ex vivo retina – ‘Chapter 19:Analysis of Gene Function in the Reina
- PSD95β regulates plasma membrane Ca(2+) pump localization at the photoreceptor synapse
- The Caudal Migratory Stream, The Caudal Migratory Stream: A Novel Migratory Stream ofInterneurons Derived from the Caudal Ganglionic Eminence in the Developing Mouse Forebrain

 

Small Tissue:
Electrode Recommendations:
-           CUY700 series and
-           CUY701 series
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe that the electroporation methods described in:
Electroporation-mediated gene transfer system applied to cultured CNS neurons
Early subdivisions in the neural plate define distinct comptence for inductive signals
are appropriate, that is, placing an electrode on the top of a tissue which is on another electrode.  A number of electrode option recommendations are appropriate:

Electrode Option 1:

-           CUY700P2E and CUY700P1L or CUY195P0.5

Electrode Option 2:

-          CUY700P2E and CUY614 with CUY615, one CUY613P1 or CUY611P3-1

 

See also Mouse and Rat Tissue/Brain Slice Recommendations


© Copyright 2017 Sonidel Limited. All Rights Reserved. XML Sitemap : User Sitemap