Zebrafish_Electroporation
ZEBRAFISH ELECTROPORATION
Zebrafish Retina
Publications:
– Inhiitbion of Muller glial cell division blocks regeneration of the light-damaged zebrafish retina
Zebrafish Muscle
Publications:
Zebrafish Fin
Publications:
Zebrafish Brain
Publications:
Electrode Recommendations:
– CUY650P3
– CUY520P5
– CU500
Ex Vivo Tissues
In Vivo Fish
– Inhibition of Müller glial cell division blocks regeneration of the light-damaged zebrafish retina
Re: In Vivo Zebrafish EP
Please note the following links to Video footage of NEPA21 Applications – the Zebrafish Video might be of particular interest:
– Direct delivery of MIF Morpholinos into Zebrafish Embryo
Please note the Zebrafish video referred to above demonstrates the NEPA21 technique used in the article: ‘Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development (Katie E. Holmes1, Matthew J. Wyatt2, Yu-chi Shen2, Deborah A. Thompson2, 3, Kate F. Barald2, 4
1Department of Veterinary Science, University of Wisconsin, Madison, 2Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, 3Present address: Department of Pulmonary Medicine, University of Michigan, Ann Arbor, MI, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI).
APPLICATION |
PUBLICATION TITLE |
ELECTRODE |
VOLTAGE |
P ON |
P OFF |
No OF PULSES |
ZEBRAFISH BRAIN |
Zou M, De Koninck P, Neve RL, Friedrich RW,Fast gene transfer into the adult zebrafish brain by herpes simplex virus 1 (HSV-1) and electroporation: methods and optogenetic applications Front Neural Circuits. 2014 May 6;8:41. | Custom-made electrodes | Change dependent on impedance 4-6 mA | See the details in the article | ||
ZEBRAFISH RETINA |
Kustermann et al., Survival, excitability, and transfection of retinal neurons in an organotypic culture of mature zebrafish retina Cell and Tissue Research, Volume 332, Number 2, Pages 195-209, May 2008 | CUY550-10CUY701P2E | 20V | 50ms | 950ms | 5 |
Thummel et al.,Inhibition of Müller glial cell division blocks regeneration of the light-damaged zebrafish retina Developmental Neurobiology, Volume 68, Issue 3, Pages 392-408, 15 February 2008. |
75V |
50ms |
950ms |
2 |
||
ZEBRAFISH FIN |
Christen B, Robles V, Raya M, Paramonov I, Izpisúa Belmonte JC Regeneration and reprogramming compared BMC Biol. 2010 Jan 20;8:5. |
15V |
50ms |
950ms |
10 |
|
Thummel et al.,Inhibition of zebrafish fin regeneration using in vivo electroporation of morpholinos against fgfr1 and msxbDevelopmental Dynamics, Volume 235, Issue 2, Pages 336-346, February 2006. |
15V |
50sm |
950ms |
10 |
||
Bai et al.,Matrix metalloproteinase expression and function during fin regeneration in zebrafish: Analysis of MT1-MMP, MMP2 and TIMP2BMatrix Biology, Volume 24, Issue 4, Pages 247-260, June 2005. |
50ms |
950ms |
10 |
|||
ZEBRAFISH EMBRYO |
Holmes KE, Wyatt MJ, Shen YC, Thompson DA, Barald KF.Direct delivery of MIF morpholinos into the zebrafish otocyst by injection and electroporation affects inner ear development J Vis Exp. 2011 Jan 7;(47). pii: 2466. doi: 10.3791/2466. | Custom-made | 13V | 5ms | 100ms | 3 |
XENOPUS |
Sasagawa et al.,Improved mRNA Electroporation Method for Xenopus Neurula EmbryosGenesis, Volume 33, Issue 2, Pages 81-85, June 2002. | 20+/-1V2.0+/-0.2kΩ | 5ms | 95ms | 10 |