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Featured Device

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Product Code: SONIDEL SP100

Desc:Sonoporator
Applic:(hover for application)Application:
  • Delivery of plasmids to cells and tissues for gene therapy-based applications and studies.
  • Delivery of nucleic acids such as siRNA, RNAi etc. to cells and tissues for studies on control of gene expression/gene therapy.
  • Delivery of cancer chemotherapeutic agents to impermeable target cells/tissues.
  • Delivery of agents to cells to study metabolic effects.


Featured Electrode



Product Code: CUY497P4

Application:
Electrodes for Electro Cell Fusion
Description:
Micro Slide Stand Model Chamber Platinum Electrode - L80 x W4 x H5 mm, 4mm gap, Volume 1.6ml - Connected with CUY499L

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Zebrafish Retina Electrode Recommendation

Electrode Recommendation:

For Adult Zebrafish Retina and where the eye is left for a while after the electroporation we recommend:

-          CUY650P3 Download Technical Drawing

For Larval Zebrafish Retina (which is quite small, 10microns) it may not be possible to inject DNA buffer.   Accordingly, we suggest that electroporation is performed after the larval zebrafish eye is taken out.  Once this is done and one intends culturing the retinae, then we recommend our petridish-type electrode.  The following configurations are possible:

-          CUY701P2E and CUY611P3-1, or

-          CUY701P2E and CUY614 w/CUY615, or

-          CUY700P2E and CUY611P3-1, or

-          CUY700P2E and CUY614 w/ CUY615

Technical Drawing CUY701P2E

Technical Drawing: CUY611P3-1

Technical Drawing: CUY700P2E

Technical Drawing: CUY614 and CUY615

Protocol Recommendation:

Kindly note this link to further Zebrafish Retina infomation.  This link refers to the work of Dr. Thummel of the University of Notre Dame who is working on the delivery of gens into adult zebrafish retina by electroporation.  In the experiment, as the adult zebrafish is used and the eye is left for a while after electroporation, CUY650P3 (tweezers-type electrode) is used.

 

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