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Featured Device

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Product Code: SONIDEL SP100

Desc:Sonoporator
Applic:(hover for application)Application:
  • Delivery of plasmids to cells and tissues for gene therapy-based applications and studies.
  • Delivery of nucleic acids such as siRNA, RNAi etc. to cells and tissues for studies on control of gene expression/gene therapy.
  • Delivery of cancer chemotherapeutic agents to impermeable target cells/tissues.
  • Delivery of agents to cells to study metabolic effects.


Featured Electrode



Product Code: CUY505P5

Application:
Electrodes for Electroporation
Description:
Mouse embryos - Zygote Platinum plate electrodes on slide glass, 5mm gap, L8W5H3mm

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[Plant Seed] Direct Gene Transfer into Mature Seeds via Electroporation

[Plant Seed] Direct Gene Transfer into Mature Seeds via Electroporation

[Plant Seed] Direct Gene Transfer into Mature Seeds via Electroporation

Direct gene transfer after the vacuum treatment

For research use, patent royalty is free in Japan

Basic Method
1: Soak mature seeds in water overnight
2: Transfer the seeds into the electroporation buffer
3: Vacuum treatment
4: Electroporation on ice
5: Incubate the seeds on ice for a few hours
6: Grow the plants under the relevant favourable conditions
7: Select the transformed plants using antibiotics.

Fig. 1. Electroporation Apparatus and the Chamber (Produced by NEPA GENE Co., Ltd.)

Apparatus: NEPA21/CUY21EDIT Chamber: CUY495P10 Electroporation on ice
Fertile transgenic plants were regenerated and self-fertilized seeds were obtained in rice and wheat. Transgene integration was confirmed by Southern hybridization. Transmission of the transgene into the next generation (T1) was indicated by PCR analysis. Transient gene expression was observed in several plant species and silk worm eggs.
1: Starting materials are mature seeds
2: Transformed plants are obtained in relatively short time
3: Plant tissue culture is not necessary
4: Possibly applicable to animal cells and microorganisms

Fig. 2. Production of Transgenic Whea

GUS Gene Expression Selection by Geneticin Transformed Plants

Fig. 3. Transient GUS Gene Expression

Rice (japonica) Rice (indica) Brassica Soybean

Fig. 4. Gene transfer into Silk Worm Eggs

Takashi Hagio, Plant Genetic Engineering Unit, National Institute of Agrobiological Sciences
*Improvement research is in progress in his laboratory


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