SONIDEL Limited      :: sales@sonidel.com        :: tech-support@sonidel.com         :: Tel:  IRE, ++353 (0)1 4434358 UK, ++44 (0)20 3239 4904 USA, ++1 (617) 297 2435 .
Most Popular

NEPA21 Electroporator
ELEPO21 Electroporator
NEPA Porator
ECFG Porator
Transgenic Mouse Zygote Electroporation: NEPA21
Organoid Electroporation: NEPA21
Neuroscience & Genome Editing Publications
Hybridoma Production for Monoclonal Antibodies
Bacteria / Yeast Electroporation
SP100: Sonoporator
NEPA21: Illustrated  Applications
NEPA21: Publications by   Research Application
- Connection Cables
- CU700: Monitoring System
- CU902: Polarity Exchanger
- CUY21 EDIT Electroporator
- CUY21 EDIT-S Electroporator
- CUY21 SC Electroporator
- In Vivo, In vitro Electrodes
- Electroporation Cuvettes
- Cuvette Chamber & Stand   Holder
- Laser Thermal Microinjector
- Mechanical Vibration Units
- Ultrasounic BioMicroscope
- KTAC-4000: Sonoporator
- LF101: Cell Fusion Device
- LF201: Cell Fusion Device
- Electro Cell Fusion Electrodes
Electroporation and Electro   Cell Fusion Accessories
- Electroporators
- Publications/Protocols
- Electrodes by Product Codes

 

Product Search
Search by product code, name or application:


Full Product List


Electrode Search
Search for the most appropriate electrode by research application:

Publications/Protocols
- Sonoporation
- Electroporation
- ElectroCell Fusion
- What Is Sonoporation?

Electrode Recommendation
- Electrode Recommendations

Featured Applications
Sonoporation
SONIDEL SP100 and KTAC4000
Electroporation
CUY21 EDIT, CUY21 SC
Electro Cell Fusion
LF101 and LF201

Newsletter Subscribe

Please enter your email:


Featured Device

0
Product Code: SONIDEL SP100

Desc:Sonoporator
Applic:(hover for application)Application:
  • Delivery of plasmids to cells and tissues for gene therapy-based applications and studies.
  • Delivery of nucleic acids such as siRNA, RNAi etc. to cells and tissues for studies on control of gene expression/gene therapy.
  • Delivery of cancer chemotherapeutic agents to impermeable target cells/tissues.
  • Delivery of agents to cells to study metabolic effects.


Featured Electrode



Product Code: CUY505P5

Application:
Electrodes for Electroporation
Description:
Mouse embryos - Zygote Platinum plate electrodes on slide glass, 5mm gap, L8W5H3mm

Information
- How to order
- Contact us

In vivo gene transfer into the adult honeybee brain by using electroporation

In vivo gene transfer into the adult honeybee brain by using electroporation

NEPA21 / CUY21 Applications.

[Honeybee] In vivo gene transfer into the adult honeybee brain by using electroporation

.

In vivo electroporation

A) The necks of honeybees that had settled in the tube were fixed in place by inserting two U-shaped plastic sheets for surgical ease and later release.

B) A honeybee settled in the tube with her neck fixed prior to the operation. ……………………………………..

……….

.

C) Schematic representation of the micropipette and electrodes placed in the honeybee brain. Purple indicates micropipette filled with DNA solution, blue indicates electrodes. AN, antennae; C, compound eyes; MB, mushroom bodies; Oc, ocelli; OL, optic lobes.

D) DNA injection and electroporation with needle electrodes.

Arrows and arrowhead indicate electrodes and micropipette for DNA injection, respectively.

Scale bar: 300µm.

Fluorescent images of electroporated honeybee brain at 50V, targeting the optic lobe (A, B) and mushroom bodies (C-F).

Fluorescent signals were detected around the anode position in the brain electroporated with GFP-expressing plasmid driven by the CMV promoter (A, C, E, F) and not in those electroporated without plasmid (B, D).

(G, H) Magnified views of GFP-expressing areas shown in (A) and (F), respectively.

Positions of electrodes are indicated by circles containing + or -.

Arrowheads indicate the regions in which fluorescence was detected (A, C, E, G) and neural projections from mushroom body cells (F, H).

Frontal view (A, B, F, G, H), rear view (C, D), and top view (E).

Panels (C) and (E) show different views of the same brain.

Department of Biological Sciences, Graduate School of Science, University of Tokyo; Takekazu Kunieda and Takeo Kubo

*University of Tokyo Patent Pending

*Biochemical and Biophysical Research Communications, Volume 318, Issue 1, Pages 25-31, May 21, 2004

.

Contact Us for further information


© Copyright 2018 Sonidel Limited. All Rights Reserved. XML Sitemap : User Sitemap