NEPA21 Cell Transfection Data
NEPA21 Cell Transfection Data Listing
Cell Lines: With Electroporation Cuvettes
| Cell Line |
Description, Species |
Characteristics, etc. |
Viability |
Efficiency |
Photo |
| HeLa |
Human Cervical Carcinoma cells |
Epithelial, Immortalized |
95% |
95% |
Yes |
| HeLa-K | Human Cervical Carcinoma cells | Epithelial, Immortalized Adherent |
90% | 90% | |
| 293 (HEK293) |
Human Embryonic Kidney cells |
Epithelial , Adherent |
90% |
90% |
|
| 293T (HEK293T) |
Human Embryonic Kidney cells |
Epithelial , Adherent |
90% |
90% |
Yes |
| TIG-7 |
Human Embryonic Lung Fibroblasts |
Diploid, Normal |
89% |
76% |
Yes |
| MRC-5 | Human Embryonic Lung Fibroblasts | Diploid, Normal Adherent |
85% | 90% | Yes |
| SUSM-1 | Human Fibroblasts | Fetal Liver, Epithelial, Normal, Immortalized | 77% (FACS) |
71% (FACS) |
Yes |
| KMST-6 | Human Fibroblasts | Whole Embryo, Epithelial Normal, Immortalized |
70% | 60% | Yes |
| hTERT-BJ1 | Human Fibroblast | Normal, Immortalized, Adherent | Good | 31% (FACS) |
|
| HUVEC | Human Umbilical Vein Endothelial cells | Normal, Adherent | 70% | 75% | |
| HT1080 | Human Fibrosarcoma cells | Connective Tissue Acetabulum, Adherent |
93% | 81% | Yes |
| MIA-PaCa-2 | Human Pancreatic Carcinoma cells | Epithelial , Adherent | 80% | 77% | Yes |
| HepG2 | Human Hepatoma cells | Epithelial , Adherent | 80% | 76% | Yes |
| HuH-7 | Human Hepatoma cells | Epithelial , Adherent | 70% | 60% | Yes |
| H1299 (NCI-H1299) |
Human Lung Cancer cells | Non-Small, Epithelial Adherent |
90% | 90% | |
| HSC-2 | Human Squamous Carcinoma cells | Oral , Adherent | 90-95% | 98% | Yes |
| HSC-3 | Human Squamous Carcinoma cells | Tongue , Adherent | 90-95% | 98% | Yes |
| HSC-4 | Human Squamous Carcinoma cells | Tongue, Adherent | 80% | 34% | Yes |
| Ca9-22 | Human Squamous Carcinoma cells | Gingival, Adherent | 60% | 60% | Yes |
| HGF | Human Gingival Fibroblasts | Normal, Adherent | Good | Good | Yes |
| MCF-7 | Human Breast Cancer cells | Epithelial, Adherent | 90% | 90-95% | Yes |
| T47D | Human Breast Cancer cells | Ductal Carcinoma Epithelial, Adherent |
90% | 80-90% | |
| MCF-10A | Human Brest cells | Normal, Mammary Epithelial Adherent |
Good | Good | |
| NUGC-3 | Human Gastric Carcinoma cells | Adherent | 73% (FACS) |
68% (FACS) |
|
| A549 | Human Lung Adenocarcinoma cells | Epithelial, Adherent | 80-90% | 90% | |
| TE-1 | Human Esophageal Carcinoma cells | Adherent | 80-90% | 41% | Yes |
| TE-8 | Human Esophageal Carcinoma cells | Adherent | 80-90% | 40% | Yes |
| LNCaP | Human Prostate Carcinoma | Epithelial, Adherent | 71% (FACS) |
90% (FACS) |
|
| PC-3 | Human Prostate Cancer cells | Epithelial, Adherent | 70% | 90% | |
| RKO | Human Colorectal Carcinoma cells | Adherent | 80% | 40% | |
| SKOV-3 | Human Ovarian Carcinoma cells | Adherent | 90% | 90% | Yes |
| OVCAR-3 | Human Ovarian Carcinoma cells | Adherent | 90% | 79% | Yes |
| RMG-1 | Human Ovarian Clear Cell Adenocarcinoma |
Adherent | 97% | 67% | Yes |
| CCS | Clear Cell Sarcoma | Adherent | 47% | 55% | |
| SK-N-SH | Human Neuroblastoma cells | Epithelial, Adherent | 95% | 95% | Yes |
| SH-SY5Y | Human Neuroblastoma cells | Adherent | 60% | 90% | Yes |
| KG-1-C | Human Oligodendroglial cells | Adherent | 85% | 60% | Yes |
| A172 | Human Glioblastoma cells | Adherent | 80-90% | 70% | Yes |
| 1321N1 | Human Astrocytoma Cells | Adherent | 80% | 80% | Yes |
| HaCaT | Human Keratinocyte cells | Normal, Immortalized Adherent |
40% | 80% | Yes |
| CCD1079sk | Human Skin Fibroblasts | Adherent | 40% | 53.2% (FACS) |
Yes |
| iHAM-4 | Human Amniotic Mesenchymal cells | Adherent | 59% | 95% | |
| iHAE-7 | Human Amniotic Epithelial cells | Adherent | 70% | 40% | |
| HTR-8/Svneo | Human Trophoblast cells | Adherent | 95% | 67% | Yes |
| RPE | Retinal Pigment Epithelium cells | Adherent | 90% | 70% | |
| Jurkat | Human T-cell Leukemia cells | Round Single, Blood, Suspension | 90% | 85% | Yes |
| Nalm-6 | Human B-cell Precursor Leukemia cells | Blood, Suspension | 65% (FACS) |
63% (FACS) |
|
| MY | Human Acute Lymphocytic Leukemia cells | Myelomonocytic, Suspension | 94% (FACS) |
22% (FACS) |
|
| PALL-2 | Human Acute Leukemia cells | Acute Lymphoblastic | 60% | 27% (FACS) |
|
| USCD/AML1 | Human Leukema cells | Suspension | 50% | 50% | |
| Namalwa | Human Burkitt’s Lymphoma cells | B lymphocyte, Blood Suspension |
70% | 70-80% | |
| Raji | Human Burkitt’s Lymphoma cells | B lymphocyte, Blood Suspension |
74% (FACS) |
53% (FACS) |
|
| Mutu I | Human Burkitt Lymphoma cells | EBV Positive, Suspension | 87% (FACS) |
91% (FACS) |
Yes |
| Mutu III | Human Burkitt Lymphoma cells | EBV Positive, Suspension | 54% (FACS) |
92% (FACS) |
|
| HL-60 | Human Promyelocytic Leukemia cells | Suspension | 80% | 80% | |
| LCL | Human Burkitt’s Lymphoma cells | Immortalized, Suspension | 55% (FACS) |
43% (FACS) |
Yes |
| Ramos-BlueTM | Human Basophilic Leukemia cells | Suspension | 80% | 55% | Yes |
| KU812 | Human Basophilic Leukemia cells | Suspension | 96% (FACS) |
42% (FACS) |
|
| K562 | Human Chronic Myelogenous Leukemia cells | Suspension | 91% (FACS) |
99% (FACS) |
Yes |
| THP-1 | Human Acute Moncytic Leukemia cells | Suspension | 76% | 63% | Yes |
| NIH/3T3 | Mouse Embryonic Fibroblasts | Swiss NIH , Embryo Adherent |
74% (FACS) |
82% (FACS) |
Yes |
| 3T3-L1 | Mouse Preadipocyte | Fibroblast like | Good | 61% | Yes |
| MEF | Mouse Embryonic Fibroblasts | Adherent | 80% | 90% | Yes |
| L | Mouse Fibroblasts | Adherent | 90% | 60-70% | Yes |
| MC3T3-E1 | Mouse Osteoblastic cells | Fibroblastic, Adherent | 98% | 70-80% | Yes |
| LLc1 (LL/2) |
Lewis Lung Cell Carcinoma 1 cells | Adherent | 87% (FACS) |
81% (FACS) |
Yes |
| C2C12 | Mouse Myoblast cells | Adherent | 80% | 70% | Yes |
| 4T1 | Mouse Breast Cancer cells | Adherent | 90% | 95% | Yes |
| Colo-26 | Mouse Colon Adenocarcinoma cells | Adherent | 95% | 90% | Yes |
| MS-1 | Mouse Pancreatic Endothelial cells | Adherent | 90% | 90% | |
| ddy Mouse Endometrial cells | Fibroblast, Adherent | 60% | 80% | ||
| WR19L | Mouse T-cell Lymphoma cells | Suspension | 92% (FACS) |
60% (FACS) |
Yes |
| RAW264.7 | Mouse Macrophage-like cells | Monocyte-like, Adherent | 70% | 56% | Yes |
| MIN6 | Mouse Pancreatic Beta cells | Suspension | 57% (FACS) |
71% (FACS) |
Yes |
| MEL | Mouse Erythroleukemia cells | Suspension | 70% (FACS) |
50% (FACS) |
|
| XS106 | Mouse Dendritic Cell | Suspension | 61% | 45% | Yes |
| mDC | Mouse Myeloid Dendritic cells | Suspension | 79% (FACS) |
72% (FACS) |
|
| MC/9 | Mouse Mast cells | Suspension | 76% (FACS) |
84% (FACS) |
Yes |
| BMBa | Mouse Bone Marrow-Derived Basophils | Suspension | 45% (FACS) |
67% (FACS) |
Yes |
| TS | Mouse Trophoblast Stem cells | Adherent | 59% | 47% | Yes |
| PC12 | Rat Adrenal Pheochromocytoma cells | Adherent | 70% | 50% | Yes |
| H9c2 | Rat Ventricular Myoblasts | Skeletal Muscle-like Adherent |
71% (FACS) |
82% (FACS) |
Yes |
| REF | Rat Embryonic Fibroblasts | Adherent | 90% | 99% | Yes |
| RDF | Rat Dermal Fibroblasts | Adherent | 90% | 40% | |
| C6 | Rat Glioma cells | Fibroblast-like, GFAP Positive, Adherent | 80% | 67% | Yes |
| RSC96 | Rat Schwann cells | Adherent | 70% | 85% | Yes |
| TtT/GF | Rat Anterior Pituitary cells | Folliculostellate, Adherent | 65% | 83% | Yes |
| UMR106 | Rat Osteoblastic cells | Adherent | 80% | 70% | |
| CHO | Chinese Hamster Ovary cells | Epithelial, Adherent | 74% | 90% | Yes |
| CHO-K1 | Chinese Hamster Ovary cells | Epithelial, Adherent | 95% | 95% | Yes |
| CHO-S | Chinese Hamster Ovary cells | Epithelial, Suspension | Good | 56% (FACS) |
|
| DT40 | Chicken B cells | Suspension | 91% | 38% (FACS) |
Yes |
| MDCK | Madrin-Darby Canine Kidney cells | Epithelial-like, Adherent | 90% | 95% | Yes |
| Vero | African Green Monkey Kidney cells | Epithelial-like, Adherent | 58% | 35% | |
| SEC | Bovine T cells | Suspension | 80% | 21% (FACS) |
|
| GTS9 | Ginbuna Thymus cells | Adherent | 50% | 30% | Yes |
.
EC Cells, iPS Cells: With Electroporation Cuvettes
| Cell Line |
Description, Species | Characteristics, etc. | Viability | Efficiency | Photo |
| TT2 | Mouse TT2 ES cells | Adherent | 50-60% | 50-60% | Yes |
| ddy Mouse ES cells | Adherent | 30% | 50% | Yes | |
| Rat WDA ES-like cells | Adherent | 60% | 80% | ||
| Mouse iPS Cells | Origin: Mouse Fibroblast, Adherent | 88.8% | 47.0% | Yes |
.
Primary Cells: With Electroporation Cuvettes
| Cell Line | Description, Species | Characteristics, etc. | Viability | Efficiency | Photo |
| HASM | Human Airway Smooth Muscle cells | Primary, Adherent | 90% | 80% | Yes |
| Human Endometrial Stromal cells | Primary Adherent | 95% | 90% | Yes | |
| Human Uterine Cervical Fibroblast | Primary, Adherent | 65% | 90% | Yes | |
| Human Amniotic Mesenchymal cells | Primary, Adherent | 70% | 40% | ||
| Human T cells | Primary, Suspension | 58% (FACS) |
90% (FACS) |
Yes | |
| Mouse Cerebral Cortex Neurons (Embryonic day14) |
Primary, Adherent | 80% | 70% | Yes | |
| Mouse Hippocampal Neurons (Embryonic day 14) |
Primary, Adherent | 80% | 60% | ||
| Mouse Basal Ganglia Primordium (Embryonic day 13.5) |
Primary, Adherent | 91% (FACS) |
71% (FACS) |
||
| Mouse Neural Stem cells | Primary, Adherent | Good | 37% | Yes | |
| Mouse Microglia cells | Primary, Adherent | 60-70% | 23% | Yes | |
| MEF | Mouse Embryonic Fibroblasts | Primary, Adherent | 85% (FACS) |
58% (FACS) |
Yes |
| Mouse Embryonic Skin Fibroblasts | Primary, Adherent | 80% | 50% | Yes | |
| BMMC (BMMCs) |
Mouse Bone Marrow-Derived Mast cells | Primary, Suspension | 64% (FACS) |
78% (FACS) |
Yes |
| Mouse Stromal cells | Primary, Adherent | 90% | 30% | ||
| Mouse Hippocampal Neurons | Primary, Adherent | 60% | 80% | ||
| Rat Bulbar Neurons (Embryonic day 15) |
Primary, Adherent | 80% | 75% | Yes | |
| Rat Cerebellar Granule cells | Primary, Adherent | 70% | 80% | ||
| Rat Meningeal Fibroblasts (Postnatal day 3) |
Primary, Adherent | 90% | 95% | Yes | |
| OEC | Rat Olfactory Ensheathing cells (Postnatal week 3) |
Primary, Adherent | 93% | 46% | Yes |
| Chick Embryonic Cerebellar Granule cells (Embryonic day 11) |
Primary, Adherent | 86% | 83% | ||
| *Cell clusters Chick Embryonic Cerebellar Granule Cells (Embryonic day 13) |
*Elplant Primary, Adherent |
35% | Yes |
.
Cells in Adherence: With Adherent-Cell Electrodes
| Cell Line | Description, Species | Characteristics, etc. | Viability | Efficiency | Photo |
| SH-SY5Y | Human Neuroblastoma cells | Adherent | 90% | 50% | Yes |
| C6 | Rat Glioma cells | Fibroblast-like, GFAP Positive, Adherent | 57% | 55% | Yes |
.
Primary Cell in Adherence: With Adherent-Cell Electrodes
| Cell | Characteristics | Viability | Efficiency | Photo | |
| Human Epidermal Keratinocyte cell | Primary, Adherent | 90% | 30% | ||
| Mouse Cerebral Cortex Neurons (Embryonic day 15) (6 DIV) |
Primary, Adherent | Good | Good | Yes | |
| Mouse Hippocampal Neurons (Embryonic day 14) (4 DIV) |
Primary, Adherent | 60% | 50% | ||
| Mouse Hippocampal Neurons (Embryonic day 18) (2 DIV) |
Primary, Adherent | 85% | 54% | Yes | |
| Mouse Stromal cells (1-month cultured) |
Primary, Adherent | 90% | 50% | ||
| Mouse Glial cells (14 DIV) |
Primary, Adherent | 80% | 50% | Yes | |
| Rat Granulosa cells | Primary, Adherent | Good | 41% | ||
.
Plant Protoplasts
| Cell | Characteristics | Luciferase Activity (CPS) |
||
| Tabacco Protoplasts | 20,312 | |||