SONIDEL Limited      :: sales@sonidel.com        :: tech-support@sonidel.com         :: Tel:  IRE, ++353 (0)1 4434358 UK, ++44 (0)20 3239 4904 USA, ++1 (617) 297 2435 .
Most Popular

- New Products
- STK10: Ultrasound   Transfection Kit
- SP100: Sonoporator
- SDM-series: Microbubbles
- MB101: Microbubble
- NEPA21 Electroporator
- NEPA21: Illustrated   Applications
- NEPA21: Publications by   Research Application
- Connection Cables
- CU700: Monitoring System
- CU902: Polarity Exchanger
- CUY21 EDIT Electroporator
- CUY21 EDIT-S Electroporator
- CUY21 SC Electroporator
- In Vivo, In vitro Electrodes
- Electroporation Cuvettes
- Cuvette Chamber & Stand   Holder
- Laser Thermal Microinjector
- Mechanical Vibration Units
- Ultrasounic BioMicroscope
- KTAC-4000: Sonoporator
- LF101: Cell Fusion Device
- LF201: Cell Fusion Device
- Electro Cell Fusion Electrodes
Electroporation and Electro   Cell Fusion Accessories
- Electroporators
- Publications/Protocols
- Electrodes by Product Codes

 

Product Search
Search by product code, name or application:


Full Product List


Electrode Search
Search for the most appropriate electrode by research application:

Publications/Protocols
- Sonoporation
- Electroporation
- ElectroCell Fusion
- What Is Sonoporation?

Electrode Recommendation
- Electrode Recommendations

Featured Applications
Sonoporation
SONIDEL SP100 and KTAC4000
Electroporation
CUY21 EDIT, CUY21 SC
Electro Cell Fusion
LF101 and LF201

Newsletter Subscribe

Please enter your email:


Featured Device

3
Product Code: OCH01

Desc:Cell Holder
Applic:Electroporation and Sonoporation


Featured Electrode



Product Code: CUY647-10X5

Application:
Electrodes for Electroporation
Description:
Tweezers with Variable Gap 2 Rectangle Electrode, 10mm x 5mm - 2 Rectangle Stainless Steel Plate Electrode Plate: W10mm x D5mm

Information
- How to order
- Contact us

LTM-1000 : Laser Thermal Microinjector

LTM-1000 : Laser Thermal Microinjector

LTM 1000

The unique micro-injector capable of PLANT CELL injection.

Compatible with glass micropipettes with 0.1μm tip


NOT available for sale in the USA, UK, Germany, France and Switzerland

LTM-1000 Introduction Video Digest Version


Thumbnail

Click on image to watch on YouTube

LTM-1000 Powerpoint Presentation

Click here for Application Note and Further Information

Features

  • Capable of producing high ejection pressures (+/- 1MPa per 1℃)
  • Targets individual cell components
  • Dramatically reduces cell death
  • Multiple injections without clogging
  • Rapid and convenient set-up & operation

Stereoscope Microinjection Set-Up
Using LTM-1000

APPLICATIONS

Microinject Materials: DNA, RNA, Protein, etc.

Capillary Tip Sizes: 0.1μm or larger

Targets: Plant Cells, Fungal Cells, Mammalian Cells, Organelles

Injection into moss cells Stereoscope Microinjection Set-Up
Using LTM-1000
SPECIFICATIONS
Laser Emission Wavelength 980nm
Laser Drive System ACC, Max. 2.5A, Continuously-Variable
Optical Output 1.3W
Dimensions 170W x 325D x 120H mm
Weight 4.1kg (includes laser head)
Power 100V- 240V, 50/60Hz

Number of injection into Plant Cells with a single capillary

Injection into the female gametophyte of plant

Conventional Method(A) LTM-1000(B) Size Difference in Capillary Tips Damaged cell using conventional method pictured on Left (A),  No damage using LTM-1000 pictured on Right (B).


Injection of siRNA into the naked female gametophyte of Torenia


Injection of plasmids into a nucleus of onion epidermal cells



Injection of antibody into plant cells

Injection into Budding Yeasts

Injection into Chloroplasts of Cyanidioschyzon merolae

.

Above information provided by: Dr. Tetsuya Higashiyama, Division of Biological Science, Graduate School of Science, Nagoya University

.

STRUCTURE
“Laser-assisted Thermal-expansion Microinjection”
Patent Application Number (2004) 361448

Heat-induced expansion of liquid in a sealed capillary generates high pressure, which enables injection by capillaries with the tip diameter around 0.1m (Knoblauch et al., Nature Biotech., 1999).
As the heat source, we chose a laser beam which enabled us to directly heat hydrophobic liquid containing laser absorber through the capillary glass and to precisely control the injection flow.

*Please be aware that LTM-1000 can not be used for Arecaceae family due to the patent license.

LTM-1000 – Laser Thermal Microinjector

SW-25 – Hydrophobic ionic liquid, 1.5ml

WF-25 – Laser beam absorbent, 1.5ml

LTM-LH – Micropipette holder

We are pleased to announce that reference of the LTM-1000, Laser Thermal Microinjector, was published in Nature.(Nature, Volume 458, Number 7236, Pages 357-361, 19 March 2009)
Defensin-like polypeptide LUREs are pollen tube attractants secreted from synergid cells
It made the front cover of the Nature!
http://www.nature.com/nature/journal/v458/n7236/covers/


© Copyright 2017 Sonidel Limited. All Rights Reserved. XML Sitemap : User Sitemap