LF201 - Electro Cell Fusion Generator

• ES/EG Cell and T-Cell (Activation or Reprogramming of
  Pluripotent Stem Cell)
• Human Dendtritic and Tumor Cells (Cancer Vaccine)
• Yeast and Mold
• Islet Cells and Tumor Cells (Transplantation of Insulin-Secreting Cell)
• Spleen and Myeloma cell fusion (ELISA)

• Plant Protoplast Fusion
• Human Monoclonal Antibody production
  (Treatment of Breast Cancer with Anti-Cancer Drug)
• Electrofusion of Recipient Oocyte and Somatic Cells
  (Bovine, Pig, Mouse and Rabbit Cloning)
• Electrofusion of Hamster or Mouse 2-Cell Blastomeres
  (Tetraploid Embryo)

• Greater control on the fusion
• Double/triple efficiency compared with PEG
• Electro cell fusion results are more stable
• No Toxicity
• Reproducibility not dependant on Researcher Skill

Features

Increased Reproducibility

To ensure accurate and reproducible cell fusion it is essential to align the
target cells prior to the fusion event. If the ion concentration of the buffer
is too high, ions will cause a water stream which will disturb the cell
alignment (pearl chain). Uniquely, the LF201 enables the researcher to
measure the resistance of the buffer prior to cell fusion and ensure the
correct ion concentration for exact cell alignment (in all experiments).
This greatly enhances reproducibility.

AC / DC Switching Time

The AC current is used to make a pearl chain (line of cells). For effective
cell fusion, it is imperative that the DC pulse is applied immediately after
the AC pulse and when the target cells are still actually in physical contact.
With the LF201, the switching time from AC pulse to DC pulse is
less than 5µsec.

Two Kinds of DC Pulse Outputs

The LF201 offers two possible DC pulse modes. A positive (+) square wave
or a bipolar (+/-) square wave. The first is a single direction pulse and in
the other the pole of the DC pulse is switched to the opposite polarity
during pulse delivery.

Post Fusion

There are two different types of post fusion AC current. One, a standard
Sine Wave (constant amplitude). The other, a Fade Wave (gradually
decreasing amplitude).

DC Pulse Output

The LF201 allows the researcher to output the DC pulse either
automatically or manually. The automatic output mode facilitates routine
work. The manual output mode enables the researcher to apply a DC
pulse while using a microscope - this is particularly useful when working
with unfamiliar cells and during nuclear transplantation.

PC Interfacing

The LF201 can be controlled via Microsoft Windows compatible software
where a cell fusion program can be entered and edited through a PC.
Each program can generate a text file capturing details such as experiment
time/date, resistance data etc..

Ease of Operation

For ease of operation, each cell fusion parameter is displayed on its own
individual LED.

Voltage

Frequency

Duration

Post Fusion AC Mode

Shunt Resistor

Resistance Measurement

AC/DC Switching Time

Memory

0-75Vrms, 1V resolution

1MHz

Pre-Fusion: 0-100sec (1sec resolution)

Post-Fusion: 0-10sec (1sec resolution)

Sine or Fade Wave*

Greater than 50Ω
Up to 39.99kΩ

Less than 5µs

99 protocols

Voltage

Pulse Length

Pulse Interval

Number of Pulses

Output

Weight

Power

Dimensions

Bipolarity Mode: Bipolar (+/-) Square Wave Pulse

0-1200V (1V resolution)

0-100µs (1µsec resolution)

0.1-10sec (0.1sec resolution)

1-100

Automatic or Manual

9kg

100V-240V

375W x 360D x 170H mm