To ensure accurate and reproducible cell fusion it is essential to align the target cells prior to the fusion event. If the ion concentration of the bufferis too high, ions will cause a water stream which will disturb the cell alignment (pearl chain). Uniquely, the LF101 enables the researcher tomeasure the resistance of the buffer prior to cell fusion and ensure the correct ion concentration for exact cell alignment (in all experiments). This greatly enhances reproducibility.
Large Choice of Electrode Configuration
A large selection of possible electrode configurations available. The electrodes have been specifically designed for the LF101 device.
Pulse Characteristics
Both automatic and manual pulse delivery (number of pulses 1-99) modes avaailable. Pulse interval can be set in increments from 0.1 - 9.9 seconds.
Voltage Settings
Voltage can be set in 1V resolutions. This is especially suitable for mammalian cloning. The device outputs a precise square waves which enhances cell fusion probability.
Ease of Operation
For ease of operation, each cell fusion parameter is displayed on its own individual LED.
DC Pulse Output
The LF101 enables the researcher to output the DC pulse either automatically or manually. The automatic output mode facilitates routinework. The manual output mode enables the researcher to apply a DCpulse while using a microscope - this is particularly useful when working with unfamiliar cells and during nuclear transplantation.
Somatic Cell & Embryo Nuclear Transplant
Specialized setting for bovine, pig, mouse, rabbit cloning. Somatic
cell and embryo nuclear transplant results in extremely high
fusion probability (e.g., more than 90% for bovine cloning).
Plant Protoplast & Mammalian Cell Fusion
Specialized setting for plant protoplast and mammalian cell fusion provide optimal cell fusion conditions. Cell fusion achieved which a
DC pulse (100V, Max) of precisely controlled voltage and pulse
range after producing the pearl chains with DC. It can elevate the rate
of hybridoma production (Cell fusion) probability.
Specifications
AC
Pulse
Wave
Shape
Sine
Wave
Voltage
0 -
50V (VP-P)
Frequency
0.1 -
3.9MHz Variable
Duration
0 -
99s
Shunt
Resistor
Greater
than 50 ohm
- Power : 100V （50/60Hz） 2A
- Size : 370W x 430D x 160H mm
- Weight : 10ｋｇ
DC
Pulse
Wave
Shape
Square
Voltage
0 -
999V
0 - 200V (1V resolution)*
Pulse
Length Range
5 -
99ms
(1ms
resolution)
Number
of Pulses
1 -
99 Automatic or Manual
Pulse
interval
0.1 -
9.9s
Shunt
Resistor
Greater
than 50 ohm
Post
fusion
Switched
On/Off
*Voltage for Cloning: 0 - 200V
Applications and Electrode Selection : Cell Fusion
