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The CUY21EDIT Electroporator is designed for In Vivo, In Ovo, In Utero and Ex Vivo Electroporation.
Because in vivo impedance (electrical resistance) is generally less stable than in vitro impedance, an accurate measurement of the target sample resistance prior to the electroporation event is the most important critical success factor for accurate and reproducibe electroporation. Fluctuations in resistance impact on delivered current values and this negatively impacts reproducibility from one EP event to the next. The CUY21EDIT automatically measures and displays the impedance of the target sample. (For example, the resistance of an EP target varies according to physical experimental factors such as the volume of the sample, the buffer used and the distance between the electrodes). In order to assure delivery of the optimal current, the researcher needs to ascertain the actual resistance of the target sample prior to the EP event. The CUY21EDIT impedance measurement function enables the researcher to check the sample impedance prior to running the EP program and adjust it if the recorded impedance value deviates from the previously recorded value. This ensures reproducibility from one EP event to the next.
Actual output voltage can be measured after the electroporation event. By checking the post EP event voltage, it is eary to determine whether the programmed voltage of the DC pulse was generated. This is a useful cross-check to establish if something malfunctioned with the device.
In our experience, the optimum voltage for most in vivo electroporations is below 100V. Accurate and low voltage square wave pulses ensure higher transfection efficiency and cause less damage to the electroporation target. While the voltage range of the CUY21EDIT can be set from 1V to 500V (in 1V increments), we have purposely designed the CUY21EDIT to output precise low voltage (below 100V) square wave pulses that do not degrade over the course of the pulse. It is this characteristic that distinguishes the CUY21EDIT from competiting devices.
The seven independent LED displays (for each of the electroporation parameters) enable program settings and measured values to be checked at a glance. For example, measured voltage and current are displayed immediately after electroporation. Separate numerical keys also make it easy for the operator to edit a saved program or set a new one.
Both pulse length and interval can be set from 0.1-999.9 msec in 0.1msec increments. The number of pulses can be programmed from 1 up to 99 times.
Over 250 different electrode configurations have been designed and optimised for use with the CUY21EDIT electroporator. Such an extensive electrode range extend the application range of the CUY21EDIT device. This is an important consideration at the point of purchase, as researchers have to be mindful that they may, in the future, wish to expand their field of interest to include other electroporation applications. Furthermore, custom-made electrodes can be easily designed and manufactured to suit your particular application of interest.
Impedance Measurement
(Ohm Check)
.
Voltage (Volt)
Pulse Length (P on)
Pulse Interval (P off)
Number of Pulses (No.)
Max. 18kΩ
1-500V (1V resolution)
0.1-999.9ms (0.1ms resolution)
0.1-999.9ms (0.1ms rsolution)
1-99
Safety limit (High Current)*..
Measured Output
(Automatic)
..
Memory
Dimensions, Weight
Power
Voltage Range Current
1 - 125V: 5.0A
125 - 250V: 2.2A
250 - 500V: 1.0A
Voltage: 1 - 500V
Current: 0.01 - 4.00A
2 programs
W390 x D450 x H250 mm, 125.4Kg
115V or 220V, 50/60Hz
* Safety function: When current exceed the limit the electroporation program is automatically shut down.
In addition to the precise current control offered by the CUY21 system, the CUY21 system also delivers a precision Square Wave throughout its pulse (unlike the decaying square wave pulses delivered by competing devices)
During electroporation, DNA naturally moves to and accumulates at the positive (+) electrode pole (similar to the process in electrophoresis). However, such accumulation (if not addressed) adversely impacts on electroporation transfection efficiency. The CU902 Polarity Exchanger addresses the sub-optimal effects of accumulation by reversing the electrical polarity of the electrodes during the electroporation event, thus preventing accumulation and ensuring better DNA uptake throughout the whole of the cells.
Mesencephalon (HH* stage 10)
Diencephalon (HH* stage 10)
CUY611 series
Head Ectoderm (HH stage 10)
Lens (HH stage 10)
Lateral Plate Mesoderm (LPM)
Somitic Mesoderm (HH stage 7-9)
Limb Bud (HH stage 7-9)
Digestive Organ (HH stage 7-9)
Ventricle (HH stage 30)
Retina (HH stage 38-42)
CUY613 series
CUY614 & CUY615
CUY613 series CUY614 & CUY615
Neural Plate (HH stage 3-7)
Cerebral Cortex, All Area (> E13.5)
Cerebral Cortex, Specific Area (> E13.5)
Forebrain Ventricle (E14)
Spinal Cord (E10.5)
CUY650P5, P7
CUY650P0.5-3, CUY650P1-5
CUY650P1-5
CUY651P
Neural Plate (Mouse: E7-13
Metencephalon (Mous: E7-13)
Somite (Mouse: E7-13)
Telencephalon (Mouse: E7-13)
CUY520P15, P20, P25
CUY650P3, P5, P10
Calf
Anterior Tibial Muscle
CUY650P3, P5 (Holding)
CUY647P10X5 (Holding)
Liver
Vessel
CUY650P10
CUY250 series combination
Testis
Ovary
CUY650P5, P10
Knee Cartilage
*CUY21SC is recommended.
Cornea
CUY670, CUY671P1
Brain Ventricle (P4-P8)
CUY650P7
CUY537P0.3, P0.5
*CUY21SC is used.
We stock over 250 different original electrode configurations specifically designed for use with the CUY21 electroporator range. This significantly expands the experimental applications range of our clients.
We also offer a customized electrode design service. We can design and manufacture electrodes to your particular research requirements.
Please feel free to contact us
If you see an electrode you want more information on, please enter the product code (e.g., CUY650P5) into the 'Search by product code, name or application' search box on the top left hand side of the webpage and click on the 'Search' button.
CUY21 Illustrated Applications
CUY21 Electrode Recommendations
CUY21 Electroporation Publications - Full List
CUY21 Electroporation Publications - by Research Interest
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