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Featured Device

2
Product Code: SONIDEL MB101

Desc:Microbubble for Ultrasound-mediated Transfection
Applic:Sonoporation Aid


Featured Electrode



Product Code: CUY580

Application:
Electrodes for Electroporation
Description:
Electrode holder for CUY550-10, CUY611 and CUY613 series electrodes

Information
- How to order
- Contact us

Plant Protoplast Fusion by Electro-Cell-Fusion and by Electroporation

Plant Protoplast Fusion

by

Electro Cell Fusion 

 

Plant Protoplast Fusion may be performed by Electro Cell Fusion using  our ECFG21 device (which has replaced the LF101 and LF201 devices).

Download: Plant Protoplast Fusion by Electro Cell Fusion Protocol

(Cell Type: General Plant Protoplast and Celery Apium Graveolens L. Fusio)

The stated methodologies illustrate the use of our LF201 or LF101 devices (now replaced by the ECFG21 device) and a chamber                    

 

Electro Cell Fusion Electrode Recommendation:

According to the protocol:

–          CUY501G4 (160ul)                             

–          CUY498-4 (2.0ml)

–          CUY498-10 (4.0ml)

are appropriate chambers. The number in brackets indicates the volume between electrodes.  If one has a preference for the larger volume chamber electrode, then we recommend the CUY498-10 electrode.

 

Further electrode information:

Our electrodes are compatible with the BTX ECM2001. However, they cannot be used with Eppendorf device.

  1. If using our electrodes with another device, both the C115 and C117 connection cables are required.
  2. Unlike the NEPA21/CUY21EDIT electroporators, the LF101, LF201 and ECFG21 do not have an automatic safety cut-off switch. (Unlike in in vivo electroporation, the researcher does not need to hold the electrodes in cell fusion). Compared with that of NEPA21/CUY21EDIT, the DC pulse length of the LF101, LF201 and ECFG21 is very short (usec).

 

 

Re: Plant Seed Electroporation

Link to further information on our webstie: Plant Seed Transfection by Electroporation

The NEPA21 electroporator and the CUY495P15 electrode can be used for this protocol.

 

Please also note that you will also need to source the following items directly from these suppliers:

           Dry Vacuum Pump (e.g. http://www.ulvac-kiko.com/en/pumps/da060d.html )

           Vacuum Desiccator (e.g. VM http://www.justis.as-1.co.jp/jus-tis/web/DirectSearch.aspx?shcode=1-5800-01

If the seed is hard, DNA cannot be transferred.

Parameter setting for the following are available to our clients:

–  Protoplast (using our 2mm-gap Cuvette)

  Plant Embryo EP (using our CUY520P5 electrode)

–  Isolated Chloroplasts (using our 2mm-gap Cuvette)

 

Related Information:

 Algae Transformation without cell-wall removal

 ECFG21 device for Hybridoma Production/Nuclear Transfer

ECFG Illustrated Applications



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