Controlling Electrofusion – LF101 & LF201
Recommended Protocol:
Kindly note the following article and Video Clip:
–  ‘Timing controllable electrofusion device for aqueous dreplet-based microreactors’
The article details an electrofusion device (LF101 or LF201) for controlling the precise moment of fusion between droplets (β-galactosidase and fluorescein di-β-D-galactopyranoside (FDG)) by applying an electric field. This device allows:
–Â Â Accurate determination of the start of chemical/biological reactions
–Â Â Minimum contact of reactants with channel walls-eliminating surface absorption problems
–Â Â Easy fabrication
–Â Â Continuous observation of initiated reaction, and
–Â Â Enzymatic reaction using fluorescence microscopy was observed