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LTM-1000
Laser Thermal Microinjector

The high pressure ensures to use capillaries with fine tips around 0.1-0.5μm for injection.

This world first microinjector enables to inject into the cell having rigid cell wall such as plant cells.

LTM-1000
K:miyamuraLTM-1000写真LTM-1000背景無.jpg

LTM-1000

*LTM-1000 does not include capillaries.

 

Application

Microinject materials (DNA, RNA, Protein, etc) into the following using capillary 0.1-0.5μm without clogging.

Plant Cells

Fungi Cells

Animal small cells

Organelles

 

http://www.nepagene.jp/catalogue/img_prdcts/LTM-1000b.jpg

Microinjection using LTM-1000

Supported by the project to develop "innovative seeds" of Japan Science and Technology Agency (JST)

 

Features

Extremely-high pressure (approx. 1MPa per 1℃) makes it possible to use capillaries with the tip around 0.1-0.5μm.

It ensures the following.

Target a cell component pointedly. (Nucleus, Mitochondrion, Chloroplast, etc)

Reduce cell damage drastically.

Inject into many cells in a single capillary. (e.g. more than 50 tobacco BY-2 cells could be injected)

Simple flow path makes it possible to start experiments right away saving a lot of labor.

 

Specifications

 

Laser emission wavelength

980nm

Laser drive system

ACC, max. 2.5A, continuously-variable

Temperature control

Peltier system 25.0℃-40.0℃
(0.1℃ increments) continuously-variable

Optical output

1.3W

Dimension

170W×325D×120H mm

Weight

4.1kg (includes laser head)

Power supply

 

 

 

Number of injection into tobacco BY-2 cells with a single capillary

LTM-1000

 

Conventional

method

 
C:UsersSakihei FujiwaraPictures図5.png

 

 

 

C:UsersUSERPictures針先端.png

 

Injection of siRNA into the naked female gametophyte of Torenia.

 

Injection of plasmids into a nucleus of onion epidermal cells

 

Injection of antibody into plant cells

 

http://www.nepagene.jp/catalogue/img_prdcts/LTM-1000-01.jpghttp://www.nepagene.jp/catalogue/img_prdcts/LTM-1000-02.jpg

http://www.nepagene.jp/catalogue/img_prdcts/LTM-1000-03.jpg

http://www.nepagene.jp/catalogue/img_prdcts/LTM-1000-04.jpg

 

 

Injection into Budding Yeasts

 

Injection into Chloroplasts of Cyanidioschyzon merolae

 

Injection into the female gametophyte of plant

 

Conventional

method

LTM-1000

 

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Division of Biological Science, Graduate School of Science, Nagoya University: Tetsuya Higashiyama

 

Structure

"Laser-assisted Thermal-expansion Microinjection"

(patent application number (2004) 361448)

C:UsersSakihei FujiwaraPictures図1.pngC:UsersSakihei FujiwaraPictures図6.png

Heat-induced expansion of liquid in a sealed capillary generates high pressure, which enables injection by capillaries with the tip diameter around 0.1μm (Knoblauch et al., Nature Biotech., 1999).

As the heat source, we chose laser beam which enabled us to directly heat hydrophobic liquid containing laser absorber through the capillary glass and to precisely control the injection flow.

 

DOWNLOAD LTM1000 POWERPOINT PRESENTATION


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