SONIDEL Limited      :: sales@sonidel.com        :: tech-support@sonidel.com         :: Tel:  IRE, ++353 (0)1 4434358 UK, ++44 (0)20 3239 4904 USA, ++1 (617) 297 2435 .
Most Popular

NEPA21 Electroporator
ELEPO21 Electroporator
NEPA Porator
ECFG Porator
Transgenic Mouse Zygote Electroporation: NEPA21
Organoid Electroporation: NEPA21
Neuroscience & Genome Editing Publications
Hybridoma Production for Monoclonal Antibodies
Bacteria / Yeast Electroporation
SP100: Sonoporator
NEPA21: Illustrated  Applications
NEPA21: Publications by   Research Application
- Connection Cables
- CU700: Monitoring System
- CU902: Polarity Exchanger
- CUY21 EDIT Electroporator
- CUY21 EDIT-S Electroporator
- CUY21 SC Electroporator
- In Vivo, In vitro Electrodes
- Electroporation Cuvettes
- Cuvette Chamber & Stand   Holder
- Laser Thermal Microinjector
- Mechanical Vibration Units
- Ultrasounic BioMicroscope
- KTAC-4000: Sonoporator
- LF101: Cell Fusion Device
- LF201: Cell Fusion Device
- Electro Cell Fusion Electrodes
Electroporation and Electro   Cell Fusion Accessories
- Electroporators
- Publications/Protocols
- Electrodes by Product Codes

 

Product Search
Search by product code, name or application:


Full Product List


Electrode Search
Search for the most appropriate electrode by research application:

Publications/Protocols
- Sonoporation
- Electroporation
- ElectroCell Fusion
- What Is Sonoporation?

Electrode Recommendation
- Electrode Recommendations

Featured Applications
Sonoporation
SONIDEL SP100 and KTAC4000
Electroporation
CUY21 EDIT, CUY21 SC
Electro Cell Fusion
LF101 and LF201


Featured Device

3
Product Code: OCH01

Desc:Cell Holder
Applic:Electroporation and Sonoporation


Featured Electrode



Product Code: CUY5000P2

Application:
Electrodes for Electro Cell Fusion
Description:
Platinum wire electrodes on petridish, 2mm gap

Information
- How to order
- Contact us
Top » Product Catalogue » Cell Fusion Generators

Electro Cell Fusion Devices
Cell Fusion can be performed not only by PEG but also by an Electro Cell Fusion device. Not many researchers are aware that Cell Fusion can be performed by a device! Our LF101 and LF201 devices have been designed for this purpose.

PEG v LF201/LF101 Device

 
LF210 ElectroCell Fusion Device

 

While the majority of researchers employ PEG, there are nevertheless problems associated with such an approach. They include:
- a lack of control on the fusion
- reproducibility being dependant on the skills of the individual researcher
- additionally, PEG presents problems with toxicity
- it can be done by a device

The particular advantage of the LF201/LF101 Device is that:
- in the case of electrofusion and the level of control afforded by the LF201/LF101, the PEG problems do not arise.
- indeed, the LF201/LF101 offers a broad range of capabilities in terms of Electro Fusion for Hybridoma Production

LF201 v LF101

The LF201 has a higher 3ification than the LF101 – it is the next generation model in the series. So, not only does the LF201 perform all the applications that can be done by LF101; it is a high-though-put Electro Cell Fusion Generator. Its wider voltage range enables it to handle a greater number of cells (in its larger chamber) at the same time. In addition, new functions have been added: resistance measurement, bi-polar pulse and attenuating post-fusion AC current etc..

LF101 and LF210 can be use for Nuclear Transplant (Cloning)/Cell Fusion

LF201 v LF101 v Competing Devices

LF201 v Competing Devices

Use of a low voltage is an imperative requirement if one is to obtain successful nuclear transplant. Unlike the LF101 and LF201 many competing devices cannot accurately control voltages in the lower ranges (10V increment setting).

LF201 v LF101

For successful and efficient cell fusion, researchers need to be able to simultaneously handle as large a volume of cells as possible. This meant increasing the size of the cell-fusion chamber from a 2mm gap (in the LF101) to a 3mm gap (in the LF201). Of necessity, a larger gap requires a higher voltage. Hence, why the LF201’s DC voltage range is 1200V and that of the LF101 is 930V. The difference between the two is that the LF201 is designed for the high-through-put cell fusion. In addition, please note the following electrode configurations and switches that support the LF201 and LF101 devices: Note also the system developed by Dr. Takeuchi of Tokyo University. The movie clip (download below) demonstrates an electrofusion device for controlling the precise moment of fusion between droplets by applying an electric field. This device allows:
1. accurate determination of the start of chemical/biological reactions
2. minimum contact of reactants with channel walls – eliminating surface absorption problems
3. easy fabrication and
4. continuous observation of initiated reaction
The movie clip demonstrates the fusion of ß-galactosidase and fluorescein di-ß-D galactopyranoside (FDG) droplets, and observed the enzymatic reaction using fluorescence microscopy. In addition, sequential fusion of pico-litre droplets was also accomplished.

Download Clip

SONIDEL Limited is please to offer the choice of both the LF101 and LF201 electro cell fusion devices:

Electrodes for Electro Cell Fusion

Electro Cell Fusion Publications / Protocols



Categories

© Copyright 2024 Sonidel Limited. All Rights Reserved. XML Sitemap : User Sitemap