Zebrafish_Electroporation

Please note the Zebrafish video referred to above demonstrates the NEPA21 technique used in the article: ‘Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development (Katie E. Holmes¹, Matthew J. Wyatt², Yu-chi Shen², Deborah A. Thompson^{2, 3}, Kate F. Barald^{2, 4}

¹Department of Veterinary Science, University of Wisconsin, Madison, ²Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, ³Present address: Department of Pulmonary Medicine, University of Michigan, Ann Arbor, MI, ⁴Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI).

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ZEBRAFISH BRAIN	Zou M, De Koninck P, Neve RL, Friedrich RW,Fast gene transfer into the adult zebrafish brain by herpes simplex virus 1 (HSV-1) and electroporation: methods and optogenetic applications Front Neural Circuits. 2014 May 6;8:41.	Custom-made electrodes	Change dependent on impedance 4-6 mA	See the details in the article

ZEBRAFISH RETINA	Kustermann et al., Survival, excitability, and transfection of retinal neurons in an organotypic culture of mature zebrafish retina Cell and Tissue Research, Volume 332, Number 2, Pages 195-209, May 2008	CUY550-10CUY701P2E	20V	50ms	950ms	5
	Thummel et al.,Inhibition of Müller glial cell division blocks regeneration of the light-damaged zebrafish retina Developmental Neurobiology, Volume 68, Issue 3, Pages 392-408, 15 February 2008.	CUY650P3	75V	50ms	950ms	2

ZEBRAFISH FIN	Christen B, Robles V, Raya M, Paramonov I, Izpisúa Belmonte JC Regeneration and reprogramming compared BMC Biol. 2010 Jan 20;8:5.	CUY647-5X10	15V	50ms	950ms	10
	Thummel et al.,Inhibition of zebrafish fin regeneration using in vivo electroporation of morpholinos against fgfr1 and msxbDevelopmental Dynamics, Volume 235, Issue 2, Pages 336-346, February 2006.	CUY650P3	15V	50sm	950ms	10
	Bai et al.,Matrix metalloproteinase expression and function during fin regeneration in zebrafish: Analysis of MT1-MMP, MMP2 and TIMP2BMatrix Biology, Volume 24, Issue 4, Pages 247-260, June 2005.	CUY650P3		50ms	950ms	10

ZEBRAFISH EMBRYO	Holmes KE, Wyatt MJ, Shen YC, Thompson DA, Barald KF.Direct delivery of MIF morpholinos into the zebrafish otocyst by injection and electroporation affects inner ear development J Vis Exp. 2011 Jan 7;(47). pii: 2466. doi: 10.3791/2466.	Custom-made	13V	5ms	100ms	3

XENOPUS	Sasagawa et al.,Improved mRNA Electroporation Method for Xenopus Neurula EmbryosGenesis, Volume 33, Issue 2, Pages 81-85, June 2002.	CUY700P20E CUY195P0.5	20+/-1V2.0+/-0.2kΩ	5ms	95ms	10