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Product Code: SONIDEL SP100

Desc:Sonoporator
Applic:(hover for application)Application:
  • Delivery of plasmids to cells and tissues for gene therapy-based applications and studies.
  • Delivery of nucleic acids such as siRNA, RNAi etc. to cells and tissues for studies on control of gene expression/gene therapy.
  • Delivery of cancer chemotherapeutic agents to impermeable target cells/tissues.
  • Delivery of agents to cells to study metabolic effects.


Featured Electrode



Product Code: CUY513-5

Application:
Electrodes for Electroporation
Description:
Stainless Steel, Electrodes for adhesive cells w/3 plates, 5mm gap

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Novel 2-Step Pulse Regime Electroporation System for In Vivo and In Vitro Applicaitons – including cells in adherence and cells in suspension

07 March 2011

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Sonoporation Cuvette Electroporation Cell Data Cell Images Electrodes Publications Applications SP100

Novel 2-Step Pulse Regime Electroporation System for In Vivo and In Vitro (including cells in adherence and cells in suspension) Applications


The NEPA21 Electroporator is our latest and most sophisticated electroporation device to-date.  It is the successor to the hugely popular CUY21SC and CUY21EDIT electroporator range.
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Luciferase expression in muscle and tumour tissues1

NEPA21

The NEPA21 Electroporator is based on the same platform and core technology that made the CUY21EDIT and CUY21SC devices leaders in their class.  The major innovations in the NEPA21system are its novel 2-Step Pulse regime and polarity exchange capability.

2-STEP PULSE REGIME AND POLARITY EXCHANGE:

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The 2-Step pulse regime with voltage decay and polarity exchange facilitates more highly controlled electroporation  pulse delivery.

This results in higher transfection efficiency and higher cell viability.

Step 1 Pore-forming Pulse Mode:
High Voltage, Short Duration, Single/Multiple Pulse(s), Voltage Decay

This poration  pulse is for forming pores in the cell membrane with minimum residual cell damage
Step 2-1
Transfer Pulse Mode:
Low Voltage, Long Duration, Multiple Pulses, Voltage Decay.

This transfer pulse is for delivering the target molecules (DNA, RNA, etc.) into cells with minimum residual cell damage
Step 2-2

Polarity Exchanged Transfer Pulse:


This can improve the transfection efficiency and again, minimise residual cell damage


Conscious that excellent transfection efficiency and cell viability is achievable using the Single-Step pulse regimes delivered by the CUY21SC and CUY21EDIT systems and that this performance is still the market-leading benchmark performance standard, we have ensured that the NEPA21 device can also deliver the Single-Step pulse regimes of those systems.

The effect of this innovation is that the NEPA21 system not only incorporates the full In Vivo, In Utero, In Ovo and Ex Vivo application ranges of the original CUY21EDIT and CUY21SC devices, but it also includes a completely new novel In Vitro (Suspended Cells and Adherent Cell)  application range.
Moreover, the NEPA21 system also affords the user considerably lower operating-cost advantages for Suspended Cell and Adherent Cell (in commercially available cell culture plates) applications as the system is designed such that there is no requirement for expensive specialised reagents.

DISPOSABLE KIT COMPARISONS:

NEPA21 (Nepa Gene) Device: N (Company L) Device: N (Company I)
NEPA ElectroporationCuvette (50 pieces)

Euro 90.00

N Kit (10 RCT)


$160.00

N Transfection Kit (25 tips)

Euro 375.00

One Electroporation


Euro 1.80

One Electroporation


$16.00

One Electroporation


Euro 15.00

*Euro Price
For many of our clients the NEPA21′s lower running cost is a major factor in the decision to purchase the device.

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APPLICATIONS CATEGORIES AND EXAMPLES:

APPLICATION CATEGORIES              and           EXAMPLES
IN VITRO
Cell Culture

DIFFICULT TO TRANSFECT CELLS:

–    Primary Cells

o    BMMC Primary Mouse Bone Marrow-Derived

Mast cells

o    Primary Mouse Neurons, Cerebral Cortex
o    Primary Rat Bulbar Nerve Cells

o    HASM  Primary Human Airway Smooth

Muscle cells

o    Primary Human Endometrial Stromal Cells

–    Neurons
–    Stem Cells
–    Blood Cells
–    Immune Cells
–    Etc

NEPA21 Cell Image Results

Cell Transfection Data

Adherent

PRIMARY CELLS:
–    Cultured Primary Neurons in adherence
–    MOUSE EMBRYO (E15) CEREBRAL CORTEX


Adherent Cell Results

Tissues/Organs (Ex Vivo)

TISSUES/ORGANS:
–    Tissue Slice
–    Brain Slices
–    Organ
–    Whole Embryo
–    Etc

IN VIVO
In Vivo: Mouse/Rat

MOUSE/RAT:
–    Brain
–    Eye
–    Muscle
–    Skin
–    Kidney
–    Liver
–    Tests
–    Embryo
–    Etc

In Utero: Mouse/Rat Embryo

MOUSE/RAT EMBRYO:
–    Cerebral
–    Cortex
–    Hippocampus
–    Spinal Cord
–    Etc

In Ovo: Chick Embryo

CHICK EMBRYO:
–    Neural Tube
–    Limb Bud
–    Digestive Organ
–    Etc


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