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Product Code: SONIDEL STK10

Desc:Ultrasound Transfection Positive Control
Applic:Sonoporation Transfection Kit


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Product Code: CUY500G2

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Gold wire electrodes on microscope slide, 2mm gap, 3/pkg

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Direct Gene Transfer into Mature Seeds via Electroporation

Direct Gene Transfer into Mature Seeds via Electroporation


NEPA21 and CUY21 Applications

.

Direct Gene Transfer into Mature Seeds via Electroporation

.

– Direct gene transfer after vacuum treatment.

– For research use, patent royalty is free in Japan.

– Authors are ready for patent licensing negotiation.


Basic Method

1. Soak mature seeds in water overnight.

2. Transfer the seeds into electroporation buffer.

3. Vacuum treatment.

4. Electroporation on ice.

5. Incubate the seeds on ice for a few hours.

6. Grow the plants under the favorable conditions.

7. Select the transformed plants using antibiotics.

Fig. 1. Electroporation Apparatus and the Chamber (Produced by NEPA GENE Co., Ltd.)

Apparatus - CUY21EDIT

Chamber - CUY495P10

Electroporation on ice

Apparatus: CUY21EDIT/NEPA21

Chamber: CUY495P10

Electroporation on ice

Fertile transgenic plants were regenerated and self-fertilized seeds were obtained in rice and wheat. Transgene integration was confirmed by Southern hybridization. Transmission of the transgene into the next generation (T1) was indicated by PCR analysis. Transient gene expression was observed in several plant species and silk worm eggs.

1.Starting materials are mature seeds.

2.Transformed plants are obtained in relatively short time.

3.Plant tissue culture is not necessary.

4.Possibly applicable to animal cells and microorganisms.

Fig. 2. Production of Transgenic Wheat GUS gene expression

Selection by Geneticin

Transformed Plants.

 GUS Gene ExpressionSelection by GeneticinTransformed Plants


Fig. 3. Transient GUS Gene Expression

Transient GUS Gene Expression

Rice (japonica)…………………………. Rice (indica) ………….. Brassica

……….Soybean


Fig. 4.Gene transfer into Silk Worm Eggs

 GFP Expression Fluorescence

Plant Genetic Engineering Unit, National Institute of Agrobiological Sciences; Takashi Hagio

*Improvement research is in progress in his laboratory.

 

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