Vacuum & Electrical Pulse Method for Plant Seed Electroporation

 Vacuum & Electrical Pulse 

	Direct gene transfer after the vacuum treatment. Patent:”Electroporation method including the use of depressurization / presurization” PCT/JP03/08937		Basic Method Soak mature seeds in water overnight Transfer the seeds into electroporation buffer Vacuum treatment Electroporation on ice Incubate the seeds on ice for a few hours Grow the plants under favorable conditions Select the transformed plants using antibiotics.
	Vacuum Chamber & CUY21EDIT		Chamber: CUY495P10	Electroporation on ice

                                 N: Negative control, –: Buffer only, V: Vacuum, E: Electroporation      GUS gene expression reference (Wheat)The level of GUS gene expression by fluorescene assay (Wheat)　　　　                                       　　         Not Transformed               npt II gene introduced                    Next Generation (T1) Individual (Rice Plant)   Geneticine Selection of Rice Seeds and/or Seedings　 Plant Genetic Engineering Unit, National Institute of Agrobiological Sciences; Takashi Hagio   * Improvement research is in progress in Dr. Hagio’s laboratory.

Gene Transfer System for Plant Seed, Egg & Mircobe

SONIDEL Limited

1. Current Agrobacterium & Microinjection Methods – Disadvantages
2. New Vacuum & Electroporation Method – Advantages
3. New Vacuum & Electroporation Method – Basic Methodology Explained
4. New Vacuum & Electroporation Method – Apparatus Required
5. New Vacuum & Electroporation Methodology – Effectiveness
6. Transformed Rice Plant Cultivation
7. Transformed Rice Plant Cultivation – Results
8. Gene Transfer to Silk Worm Eggs
9. Gene Transfer to Arabidopsis Seed
10. Gene Transfer to Yest
11. New Vacuum & Electroporation Method – Improvement