SONIDEL Limited      :: sales@sonidel.com        :: tech-support@sonidel.com         :: Tel:  IRE, ++353 (0)1 4434358 UK, ++44 (0)20 3239 4904 USA, ++1 (617) 297 2435 .
Most Popular

NEPA21 Electroporator
ELEPO21 Electroporator
NEPA Porator
ECFG Porator
Transgenic Mouse Zygote Electroporation: NEPA21
Organoid Electroporation: NEPA21
Neuroscience & Genome Editing Publications
Hybridoma Production for Monoclonal Antibodies
Bacteria / Yeast Electroporation
SP100: Sonoporator
NEPA21: Illustrated  Applications
NEPA21: Publications by   Research Application
- Connection Cables
- CU700: Monitoring System
- CU902: Polarity Exchanger
- CUY21 EDIT Electroporator
- CUY21 EDIT-S Electroporator
- CUY21 SC Electroporator
- In Vivo, In vitro Electrodes
- Electroporation Cuvettes
- Cuvette Chamber & Stand   Holder
- Laser Thermal Microinjector
- Mechanical Vibration Units
- Ultrasounic BioMicroscope
- KTAC-4000: Sonoporator
- LF101: Cell Fusion Device
- LF201: Cell Fusion Device
- Electro Cell Fusion Electrodes
Electroporation and Electro   Cell Fusion Accessories
- Electroporators
- Publications/Protocols
- Electrodes by Product Codes

 

Product Search
Search by product code, name or application:


Full Product List
$catmenu
Electrode Search
Search for the most appropriate electrode by research application:

Publications/Protocols
- Sonoporation
- Electroporation
- ElectroCell Fusion
- What Is Sonoporation?

Electrode Recommendation
- Electrode Recommendations

Featured Applications
Sonoporation
SONIDEL SP100 and KTAC4000
Electroporation
CUY21 EDIT, CUY21 SC
Electro Cell Fusion
LF101 and LF201


Featured Device

3
Product Code: OCH01

Desc:Cell Holder
Applic:Electroporation and Sonoporation


Featured Electrode



Product Code: CUY499

Application:
Electrodes for Electro Cell Fusion
Description:
Container for Micro Slide Stand Model Chamber (Glass at bottom, For microscope observation) - L125 x W80 x H40 mm - Connected with CUY498-3, 4, 10, P10

Information
- How to order
- Contact us

Knock-down by transfection of shRNA expression vector by electroporation

Knock-down by transfection of shRNA expression vector by electroporation

NEPA21 / CUY21 Applications

[In Ovo] Knock-down by transfection of shRNA expression vector by electroporation


(A) shRNA expression vector is electroporated as shown above.

(B) Select target DNA sequence of 19 to 21 mer. Sense and antisense sequence were linked to a nucleotide spacer as a loop and put into expression vector that is driven by U6 or H1 promoter. Commercially available expression vector that dreives expression by mouse U6 promoter is effective in chick embryos.

(C) After transcription, hairpin is digested to form siRNA, and siRNA forms RISK (RNA-induced silencing complex) to degrade the target mRNA.

.

shRNA expression 24 hous after electroporation.

24 hours after electroporation.

(A) Transfection is monitored by co-electroporated GFP fluorescence.

(B), (C) Application of siRNA against En2 by electroporation shows degradation of En2 mRNA (B).

(C) Control side

.

Graduate School of Life Sciences, and Institute of Development, Aging and Cancer, Tohoku University

Harukazu Nakamura and Tatsuya Katahira

*Mechanisms of Development, Volume 121, Issue 9, Pages 1137-1143, September 2004

*Development Growth & Differentiation, Volume 45, Issue 4, Pages 361-367, August 2003

.

.

Contact Us for more Information


© Copyright 2024 Sonidel Limited. All Rights Reserved. XML Sitemap : User Sitemap