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Featured Device

2
Product Code: SONIDEL MB101

Desc:Microbubble for Ultrasound-mediated Transfection
Applic:Sonoporation Aid


Featured Electrode



Product Code: CUY600-6

Application:
Electrodes for Electroporation
Description:
6 x 5mm tip length needle electrodes on tip of the stick. Require CU901

Information
- How to order
- Contact us

NEPA21 Cell Image Viability and Transfection Efficiency Results

NEPA21 Cell Image Viability and Transfection Efficiency Results

Cell Lines: With Electroporation Cuvettes

HeLa

Human Cervical Carcinoma cells

.

Viability: 95%

Transfection Efficiency: 95%

.

293 (HEK293)

Human Embryonic Kidney cells

293 (HEK293) - Human Embryonic Kidney Cells - Viability 90 per cent and Transfection Efficiency 70 per cent

Viability: 90%

Transfection Efficiency: 70%

293T (HEK293T)
Human Embryonic Kidney cells

Viability: 90%

Transfection Efficiency: 95%

TIG-3

Human Embryonic Lung Fibroblasts

TIG-3 - Human Embryonic Lung Fibroblasts - Viability 90 per cent and Transfection Efficiency 80 per cent

Viability: 90%

Transfection Efficiency: 80%

*48 hours after EP
. .

TIG-7

Human Embryonic Lung Fibroblasts

. .

Viability 89%

Transfection Efficiency 76%

. .

MRC-5

Human Embryonic Lung Fibroblasts

Viability 85%

Transfection Efficiency 90%

. .

SUSM-1

Human Fibroblasts

Viability 77%

Transfection Efficiency 71%

. .

KMST-6

Human Fibroblasts

Viability 70%

Transfection Efficiency 60%

. .

HT1080

Human Fibrosarcoma cells

Viability 93%

Transfection Efficiency 81%

. ..

MIA-PaCa-2

Human Pancreatic Carcinoma cells

Viability 80%

Transfection Efficiency 77%

.. .

HepG2

Human Hepatoma cells

Viability 88%

Transfection Efficiency 76%

.
.

HuH-7

Human Hepatoma cells

Viability: 70%

Transfection Efficiency: Excellent

.

H69

Human Small-Cell Lung Cancer cells

H69 - Human Small-Cell Lung Cancer cells - Viability 90 per cent H69 - Human Small-Cell Lung Cancer cells - Transfection Efficiency 85 per cent

Viability: 90%

Transfection Efficiency: 85%

HSC-2

Human Squamous Carcinoma cells

Viability: 90-95%

Transfection Efficiency: 98%

. .

HSC-3

Human Squamous Carcinoma cells

HSC-3 - Human Squamous Carcinoma cells - Viability 90-95 per cent HSC-3 - Human Squamous Carcinoma cells- Transfection Efficiency 98 per cent

Viability: 90-95%

Transfection Efficiency: 98%

.

HSC-4

Human Squamous Carcinoma cells

Viability: 80%

Transfection Efficiency: 31%

.

Ca9-22

Human Squamous Carcinoma cells

Viability: 60%

Transfection Efficiency: 60%

.

HGF

Human Gingival Fibroblasts

Viability: Good

Transfection Efficiency: Good

.

BT-20

Human Breast Cancer cells

BT-20 - Human Breast Cancer cells - Viability 70 per cent and Transfection Efficiency 80 per cent

Viability: 70%

Transfection Efficiency: 80%

MCF-10A

Human Breast Cells

Viability: Good

Transfection Efficiency: Good

.

TE-1

Human Esophageal Carcinoma cells

Viability 85%

Transfection Efficiency 41%

. .

TE-8

Human Esophageal Carcinoma cells

Viability 85%

Transfection Efficiency 40%

.

SKOV-3

Human Ovarian Carcinoma cells

Viability: 90%

Transfection Efficiency: 90%

. .

OVCAR-3

Human Ovarian Carcinoma cells

Viability 90%

Transfection Efficiency 79%

. .

RMG-1

Human Ovarian Clear Cell Adenocarcinoma

Viability 97%

Transfection Efficiency 67%

. .

SK-N-SH

Human Neuroblastoma cells

Blue: TO-PRO Green: GFP

Viability 95%

Transfection Efficiency 95%

.

SH-SY5Y

Human Neuroblastoma cells

*Left image: Lipofectamine LTX [ Viability:90% Transfection Efficiency:10%]

Viability: 60%

Transfection Efficiency: 90%

.
.

KG-1-C

Human Oligodendroglial cells

Viability 85%

Transfection Efficiency 60%

.

A172

Human Glioblastoma cells

Viability: 85%

Transfection Efficiency: 70%

. .

1321N1

Human Astrocytoma cells

Viability: 80%

Transfection Efficiency: 80%

.

HaCaT

Human Keratinocyte cells

Viability 40%

Transfection Efficiency 80%

. .

CCD1079sk

Human Skin Fibroblasts

Viability 40%

Transfection Efficiency 53%

. .

HTR-8/Svneo

Human Trophoblast cells

Viability 95%

Transfection Efficiency 67%

UT-7/TPO

Human Megakaryocytic cells

UT-7 TPO - Human Megakaryocytic cells - Viability 51 per cent UT-7 TPO - Human Megakaryocytic cells - Transfection Efficiency 46 per cent

Viability: 51%

Transfection Efficiency: 46%

Jurkat

Human T-cell Leukemia cells

Viability: 90%

Transfection Efficiency: 85%

.

KG-1

Human Acute Myeloid Leukemia cells

KG-1 - Human Acute Myeloid Leukemia cells - Viability 60 percent KG-1 - Human Acute Myeloid Leukemia cells - Transfection Efficiency 65 percent

Viability: 60%

Transfection Efficiency: 65%

For ref. Lipofectamine 2000 (Invitrogen) Transfection Efficiency: 0%

Mutu 1

Human Burkitt Lymphoma cells

Viability: 87%

Transfection Efficiency: 91%

. .

LCL

Human Lymphoblastoid cell

Viability: 53%

Transfection Efficiency: 43%

.

Ramos-BlueTM

Human Burkitt Lymphoma cells

Viability: 80%

Transfection Efficiency: 55%

.

K562

Human Erythroleukemia cells

Viability: 91%

Transfection Efficiency: 99%

.

THP-1

Human acute monocytic leukemia cells

Viability: 76%

Transfection Efficiency: 63%

.

NIH/3T3

Mouse Embryonic Fibroblasts

Viability: 74%

Transfection Efficiency: 81%

.

3T3-L1

Mouse Preadipocytes

Viability: 90%

Transfection Efficiency: 90%

.

MEF

Mouse Embryonic Fibroblasts

Viability: 75%

Transfection Efficiency: 70%

.

P19C6

Mouse Embryonic Carcinoma cells

P19C6 - Mouse Embryonic Carcinoma cells - Viability 90 per cent P19C6 - Mouse Embryonic Carcinoma cells - Transfection Efficiency 50 per cent

Viability: 90%

Transfection Efficiency: 50%

For ref. Lipofectamine 2000 (Invitrogen) V: 55% TE: 50%

L

Mouse Fibroblasts

Viability: 90%

Transfection Efficiency: 65%

.

MC3T3-E1

Mouse Osteoblastic cells

Viability 85%

Transfection Efficiency 75%

. .

LLc1 (LL/2)

Mouse Lewis lung cell carcinoma 1 cells

Viability: 87%

Transfection Efficiency: 81%

.

C2C12

Mouse Myoblast cells

Viability: 80%

Transfection Efficiency: 70%

.

4T1

Mouse Breast Cancer cells

Viability: 90%

Transfection Efficiency: 95%

.

Colon-26

Mouse Colon Adenocarcinoma cells

Viability: 95%

Transfection Efficiency: 90%

.

ddy Mouse Endometrial cells

Viability: 60%

Transfection Efficiency: 80%

.

Neuro-2a

Mouse Neuroblastoma cells

Neuro-2a - Mouse Neuroblastoma cells - Viability 90 per cent Neuro-2a - Mouse Neuroblastoma cells - Transfection Efficiency 90 per cent

Viability: 90%

Transfection Efficiency: 90%

BV-2

Mouse Microglial cells

BV-2 - Mouse Microglial cells - Viability 92 per cent BV-2 - Mouse Microglial cells - Transfection Efficiency 60 per cent

Viability: 92%

Transfection Efficiency: 60%

WR19L

Mouse T-cell lymphoma cells

Viability: 92%

Transfection Efficiency: 60%

.

RAW264.7

Mouse Macrophage-like cells

Viability: 70%

Transfection Efficiency: 56%

.

MIN6

Mouse Pancreatic Beta cells

Viability 57%

Transfection Efficiency 71%

.

Mouse Pancreatic Islet Beta cells

Viability: 60%

Transfection Efficiency: 40%

.

XS106

Mouse Dendritic Cells

Viability 61%

Transfection Efficiency 45%

. .

MC/9

Mouse Mast cells

Viability: 76%

Transfection Efficiency: 84%

.

BMBa

Mouse Bone marrow-derived basophils

Viability: 45%

Transfection Efficiency: 67%

.

ATDC5

Mouse Pre-chondrocytic cells

Blue:DAPI Green:GFP For ref. invitrogen:Lipofectamine LTX (with Plus Reagent) Transfection Efficiency:0.001%

Viability: 62%

Transfection Efficiency: 14%

.

PC12

Rat Adrenal Pheochromocytoma cells

PC12 - Rat Adrenal Pheochromocytoma cells - Transfection Efficiency 70 per cent

Viability: 90%

Transfection Efficiency: 70%

.

H9c2

Rat Ventricular Myoblasts

Viability 71%

Transfection Efficiency 82%

. .

REF

Rat Embryonic Fibroblasts

Viability 90%

Transfection Efficiency 99%

. .

C6

Rat Glioma cells

Viability 80%

Transfection Efficiency 67%

. .

RSC96

Rat Schwann cells

Viability: 70%

Transfection Efficiency: 85%

.

TtT/GF

Rat Anterior Pituitary cells

Viability: 65%

Transfection Efficiency: 83%

.

CHO

Chinese Hamster Ovary cells

Viability 74%

Transfection Efficiency 90%

. .

CHO-K1

Chinese Hamster Ovary cells

Viability 95%

Transfection Efficiecncy 95%

. .

DT40

Chicken B cells

Viability 38%

Transfection Efficiency 40%

. .

MDCK

Madrin-Darby Canine

Viability: 90%

Transfection Efficiency: 95%

.

BFF

Bovine Fetal Fibroblasts

BFF - Bovine Fetal Fibroblasts – Viability 78 per cent BFF - Bovine Fetal Fibroblasts – Transfection Efficiency 72 per cent

Viability: 78%

Transfection Efficiency: 72%

A6

Xenopus Kidney Epithelial cells

A6 - Xenopus Kidney Epithelial cells - Viability 90 per cent A6 - Xenopus Kidney Epithelial cells - Transfection Efficiency 60 per cent

Viability: 90%

Transfection Efficiency: 60%

GTS9

Ginbuna Thymus cells

Viability: 50%

Transfection Efficiency: 30%

………………………………
. …………………….

Primary Cells: With Electroporation Cuvettes

Primary HASM

Human Airway Smooth Muscle cells

Viability 90%

Transfection Efficiency 80%

. .

Primary

Human Endometrial Stromal Cells

Viability 95%

Transfection Efficiency 90%

. .

Primary

Human Uterine Cervical Fibroblast

Viability 65%

Transfection Efficiency 90%

. .

Primary

Human T cells

The cells were cultured and stimulated with CD3 CD28 antibody for 66 hours before Electroporation

(the result of amaxa’s device: V about 20%  TE about 60%)

Viability: 58%

Transfection Efficiency: 90%

Primary

Mouse Cerebral Cortex Neurons

9 days after EP

Red: MAP2 Blue: GFAP Green: GFP The cells were from Embryonic Day 14 Mouse Cerebral Cortex

Viability 80%

Transfection Efficiency 70%

. .

Primary

Mouse Neural Stem cells

Viability: Good

Transfection Efficiency: 37%

Primary

Mouse Microglia cells

Viability 65%

Transfection Efficiency 23%

. .

Primary MEF

Mouse Embryonic Fibroblasts

Viability: 90%

Transfection Efficiency: 85%

.

Primary

Mouse Embryonic Skin Fibroblasts

Viability: 90%

Transfection Efficiency: 50%

Primary BMMC

Mouse Bone Marrow-Derived Mast Cells

Viability 64%

Transfection Efficiency 78%

. .

Primary

Mouse Liver cells

Primary - Mouse Liver cells - Viability 70 per cent Primary - Mouse Liver cells - Transfection Efficiency 50 per cent

Viability: 70%

Transfection Efficiency: 50%

Primary

Rat Cerebral Cortex Neurons

Primary - Rat Cerebral Cortex Neurons - Viability 64 per cent Primary - Rat Cerebral Cortex Neurons - Transfection Efficiency 78 per cent

Viability: 64%

Transfection Efficiency: 78%

The cells were from Embryonic Day 16 Rat Cerebral Cortex.24 hours after electroporation.

Primary

Rat Bulbar Neurons

7 days after EP / The cells were from Embryonic Day 15 Rat Bubblar Neurons

Viability 80%

Transfection Efficiency 75%

.

Primary

Rat Meningeal Fibroblasts

Viability: 90%

Transfection Efficiency: 95%

. .

Primary

Rat Meningeal Fibroblasts

Viability 90%

Transfection Efficiency 95%

. .

Primary

Rat Meningeal Fibroblasts

Blue: NG2 Green: GFP Postnatal day 3

Viability: 90%

Transfection Efficiency: 95%

.

Primary OEC

Rat Olfactory Ensheathing Cells


5 days after electroporation – The cells were from Postnatal Week 3 – Electroporation: after being cultured for 2 weeks.

Viability 93%

Transfection Efficiency 46%

. .

Primary

Chick Embryonic Fibroblasts

Primary - Chick Embryonic Fibroblasts - Viability 80 Percent and Transfection Efficiency 90 per cent.

Viability: 80%

Transfection Efficiency: 90%

Primary

Chick Embryonic Cerebellar Granule Cells

*Explant Culture (cell clusters) Embryonic day 13, **Scale: 50 µm

Viability:

Transfection Efficiency: 35%

.

Primary

Human Mesenchymal Stem cells

Primary - Human Mesenchymal Stem cells - Viability 78 per cent Primary - Human Mesenchymal Stem cells - Transfection Efficiency 75 per cent

Viability: 78%

Transfection Efficiency: 75%

Cord blood derived

Nucleofector (Lonza amaxa) result: Viability 20% Transfection Efficiency 20%

Primary - Human Mesenchymal Stem cells - Graph

TS

Mouse Trophoblast Stem cells

Viability 59%

Transfection Efficiency 47%

Mouse TT2 ES cells

Viability: 55%

Transfection Efficiency: 55%

Mouse ES cells

Viability: 80%

Transfection Efficiency: 75%

Mouse iPS cells

Viability: 89%

Transfection Efficiency: 47%

Neurospheres

Neural Stem cells Derived from Mouse SVZ

Neurospheres - Neural Stem cells Derived from Mouse SVZ - 24 hours after electroporation Neurospheres - Neural Stem cells Derived from Mouse SVZ - 48 hours after electroporation
24 hours after electroporation 48 hours after electroporation
Neurosphres - Neural Stem cells Derived from Mouse SVZ - 72 hours after electroporation
72 hours after electroporation

Cell Lines in Adherence: With Adherent-Cell Electrode

.

SH-SY5Y

Human Neuroblastoma cells

In Adherence

Viability: 90%

Transfection Efficiency: 50%

.

EPC – Human Endothelial Progenitor Cells

In Adherence

Human Endothelial Progenitor Cells (EPC) - In Adherence - Control Human Endothelial Progenitor Cells (EPC) - In Adherence - With Electroporation

Control

With Electroporation

Adherent Cell EP with CU600 holder and CUY900-13-3-5 ElectrodeCUY900-13-3-5 - Electrode for Adherent Cell Electroporation

3T3-L1 Mouse Preadipocytes

In Adherence

3T3-L1 Mouse Preadipocytes - Viability 90 per cent 3T3-L1 Mouse Preadipocytes - In Adherence - Transfection Efficiency 70 per cent

24 hours after electroporation

Electroporation: After 7 days of differentiation

Adherent Cell EP with CU600 holder and CUY900-13-3-5 ElectrodeCUY900-13-3-5 - Electrode for Adherent Cell Electroporation

NEPA21 Adherent Cell EP with CU600…………………………… CUY900-13-3-5 – Electrode

holder and CUY900-13-3-5 Electrode …………………………………for Adherent Cell EP

Viability: 90%

Transfection Efficiency: 70%

C6 Rat Glioma cells
In Adherence

Viability: 80%

Transfection Efficiency: 39%

…………………………
………………….

Primary Cells in Adherent Status: With Adherent-Cell Elelctrode

Primary Mouse Neurons
In Adherence

2 days after electroporation
The neurons were prepared from E15 mouse cerebral cortex.
Electroporation: After 6 DIV (on 24-well plates)

Viability: Excellent

Transfection Efficiency: Excellent

..

Primary Mouse Neurons In Adherence

(High magnification)

2 days after electroporation
The neurons were prepared from E15 mouse cerebral cortex.
Electroporation: After 6 DIV (on 24-well plates)
.

Primary Mouse Neurons In Adherence

(High magnification)

2 days after electroporation
The neurons were prepared from E15 mouse cerebral cortex.
Electroporation: After 6 DIV (on 24-well plates)
.

Primary Mouse Hippocampal Neurons

In Adherence

Viability: 85%

Transfection Efficiency: 54%

The neurons were prepared from E18 mouse cerebral cortex.
Electroporation: After 2 DIV (on 24-well plates)
.

Primary Mouse Glial cells

In Adherence

Electroporation: After 14 DIV

Viability: 80%

Transfection Efficiency: 50%

Primary Rat Neurons

In Adherence

Primary - Rat Neurons in Adherence - Viability 70 per cent. Primary - Rat Neurons in Adherence - Transfection Efficiency 60 per cent

Viability: 70%

Transfection Efficiency: 60%

24 hours after electroporation

The neurons were prepared from E17 rat cerebral cortex.

Electroporation: After 2 DIV (on 24-well plates)

Adherent Cell EP with CU600 holder and CUY900-13-3-5 ElectrodeCUY900-13-3-5 - Electrode for Adherent Cell Electroporation

Primary Rat Neurons

In Adherence

Primary Rat Neurons in Adherence - 48 hrs post EP - Viability 100 per cent Primary Rat Neurons in Adherence - 48 hrs post EP - Transfection Efficiency 50 per cent

Viability: 100%

Transfection Efficiency: 50%

48 hours after electroporation

The neurons were prepared from P7 rat hippocampus.

Electroporation: After 11 DIV

CUY900-13-3-5 - Adherent cell electrode

Primary Rat Neurons

In Adherence

Primary Rat Neurons in Adherence - 48 hrs post EP - Viability 100 per cent x 2 Primary Rat Neurons in Adherence - 48 hrs post EP - Transfection Efficiency 50 per cent x 2

Viability: 100%

Transfection Efficiency: 50%

48 hours after electroporation

The neurons were prepared from P7 rat hippocampus.

Electroporation: After 11 DIV

CUY900-13-3-5 - Adherent cell electrode.jpg

Rat

Brain Slices

Rat Brain Slices1 Rat Brain Slices2
48 hours after electroporation

The neurons were prepared from P7 rat hippocampus.

Electroporation: After 11 DIV

Rat Brain Slices - DiagramRat Brain Slices - Electrode

Rat

Brain Slices

Rat Brain Slices3 Rat Brain Slices4

48 hours after electroporation

The neurons were prepared from P7 rat hippocampus.

Electroporation: After 11 DIV

/Rat Brain Slices - Electrode/Rat Brain Slices - Diagram

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