HeLa

Human Cervical Carcinoma cells

Viability: 95%

Transfection Efficiency: 95%

293T (HEK293T)
Human Embryonic Kidney cells

Viability: 90%

Transfection Efficiency: 90%

TIG-7

Human Embryonic Lung Fibroblasts

Viability 89%

Transfection Efficiency 76%

MRC-5

Human Embryonic Lung Fibroblasts

Viability 85%

Transfection Efficiency 90%

SUSM-1

Human Fibroblasts

Viability 77%

Transfection Efficiency 71%

KMST-6

Human Fibroblasts

Viability 70%

Transfection Efficiency 60%

HT1080

Human Fibrosarcoma cells

Viability 93%

Transfection Efficiency 81%

MIA-PaCa-2

Human Pancreatic Carcinoma cells

Viability 80%

Transfection Efficiency 77%

HepG2

Human Hepatoma cells

Viability 88%

Transfection Efficiency 76%

HuH-7

Human Hepatoma cells

Viability: 70%

Transfection Efficiency: Good

HSC-2

Human Squamous Carcinoma cells

Viability: 90-95%

Transfection Efficiency: 98%

HSC-3

Human Squamous Carcinoma cells

Viability: 90-95%

Transfection Efficiency: 98%

HSC-4

Human Squamous Carcinoma cells

Viability: 80%

Transfection Efficiency: 31%

Ca9-22

Human Squamous Carcinoma cells

Viability: 60%

Transfection Efficiency: 60%

HGF

Human Gingival Fibroblasts

Viability: Good

Transfection Efficiency: Good

MCF-10A

Human Breast Cells

Viability: Good

Transfection Efficiency: Good

TE-1

Human Esophageal Carcinoma cells

Viability 85%

Transfection Efficiency 41%

TE-8

Human Esophageal Carcinoma cells

Viability 85%

Transfection Efficiency 40%

SKOV-3

Human Ovarian Carcinoma cells

Viability: 90%

Transfection Efficiency: 90%

OVCAR-3

Human Ovarian Carcinoma cells

Viability 90%

Transfection Efficiency 79%

RMG-1

Human Ovarian Clear Cell Adenocarcinoma

Viability 97%

Transfection Efficiency 67%

SK-N-SH

Human Neuroblastoma cells

Viability 95%

Transfection Efficiency 95%

SH-SY5Y

Human Neuroblastoma cells

*Left image: Lipofectamine LTX [ Viability:90％ Transfection Efficiency:10％]

Viability: 60%

Transfection Efficiency: 90%

KG-1-C

Human Oligodendroglial cells

Viability 85%

Transfection Efficiency 60%

A172

Human Glioblastoma cells

Viability: 80-90%

Transfection Efficiency: 70%

1321N1

Human Astrocytoma cells

Viability: 80%

Transfection Efficiency: 80%

HaCaT

Human Keratinocyte cells

Viability 40%

Transfection Efficiency 80%

CCD1079sk

Human Skin Fibroblasts

Viability 40%

Transfection Efficiency 53%

HTR-8/Svneo

Human Trophoblast cells

Viability 95%

Transfection Efficiency 67%

Jurkat

Human T-cell Leukemia cells

Viability: 90%

Transfection Efficiency: 85%

Mutu

Human Burkitt Lymphoma cells

Viability: 87%

Transfection Efficiency: 91%

LCL

Human Lymphoblastoid cell

Viability: 41%

Transfection Efficiency: 40%

Ramos-BlueTM

Human Burkitt Lymphoma cells

Viability: 80%

Transfection Efficiency: 55%

K562

Human Erythroleukemia cells

Viability: 91%

Transfection Efficiency: 99%

THP-1

Human acute monocytic leukemia cells

Viability: 76%

Transfection Efficiency: 63%

NIH/3T3

Mouse Embryonic Fibroblasts

Viability: 74%

Transfection Efficiency: 82%

3T3-L1

Mouse Preadipocytes

Viability: 90%

Transfection Efficiency: 90%

MEF

Mouse Embryonic Fibroblasts

Viability: 95%

Transfection Efficiency: 65%

L

Mouse Fibroblasts

Viability: 90%

Transfection Efficiency: 65%

MC3T3-E1

Mouse Osteoblastic cells

Viability 85%

Transfection Efficiency 75%

LLc1 (LL/2)

Mouse Lewis lung cell carcinoma 1 cells

Viability: 87%

Transfection Efficiency: 81%

C2C12

Mouse Myoblast cells

Viability: 80%

Transfection Efficiency: 70%

4T1

Mouse Breast Cancer cells

Viability: 90%

Transfection Efficiency: 95%

Colon-26

Mouse Colon Adenocarcinoma cells

Viability: 95%

Transfection Efficiency: 90%

ddy Mouse Endometrial cells

Viability: 60%

Transfection Efficiency: 80%

WR19L

Mouse T-cell lymphoma cells

Viability: 92%

Transfection Efficiency: 60%

RAW264.7

Mouse Macrophage-like cells

Viability: 70%

Transfection Efficiency: 56%

MIN6

Mouse Pancreatic Beta cells

Viability 57%

Transfection Efficiency 71%

Mouse Pancreatic Islet Beta cells

Viability: 60%

Transfection Efficiency: 40%

XS106

Mouse Dendritic Cells

Viability 61%

Transfection Efficiency 45%

MC/9

Mouse Mast cells

Viability: 76%

Transfection Efficiency: 84%

BMBa

Mouse Bone marrow-derived basophils

Viability: 45%

Transfection Efficiency: 67%

TS

Mouse Trophoblast Stem cells

Viability 59%

Transfection Efficiency 47%

ATDC5

Mouse Pre-chondrocytic cells

Blue:DAPI Green:GFP For ref. invitrogen:Lipofectamine LTX (with Plus Reagent) Transfection Efficiency:0.001%

Viability: 62%

Transfection Efficiency: 14%

PC12

Rat Adrenal Pheochromocytoma cells

Viability: 70%

Transfection Efficiency: 50%

H9c2

Rat Ventricular Myoblasts

Viability 71%

Transfection Efficiency 82%

REF

Rat Embryonic Fibroblasts

Viability 90%

Transfection Efficiency 99%

C6

Rat Glioma cells

Viability 80%

Transfection Efficiency 67%

RSC96

Rat Schwann cells

Viability: 70%

Transfection Efficiency: 85%

TtT/GF

Rat Anterior Pituitary cells

Viability: 65%

Transfection Efficiency: 83%

CHO

Chinese Hamster Ovary cells

Viability 74%

Transfection Efficiency 90%

CHO-K1

Chinese Hamster Ovary cells

Viability 95%

Transfection Efficiecncy 95%

DT40

Chicken B cells

Viability 38%

Transfection Efficiency 40%

MDCK

Madrin-Darby Canine

Viability: 90%

Transfection Efficiency: 95%

GTS9

Ginbuna Thymus cells

Viability 50%

Transfection Efficiency 30%

Mouse TT2 ES cells

Viability: 55%

Transfection Efficiency: 55%

Mouse iPS cells

Viability: 89%

Transfection Efficiency: 47%

Primary HASM

Human Airway Smooth Muscle cells

Viability 90%

Transfection Efficiency 80%

Primary

Human Endometrial Stromal Cells

Viability 95%

Transfection Efficiency 90%

Primary

Human Uterine Cervical Fibroblast

Viability 65%

Transfection Efficiency 90%

Primary

Human T cells

The cells were cultured and stimulated with CD3 CD28 antibody for 66 hours before Electroporation

(the result of amaxa’s device: V about 20%  TE about 60%)

Viability: 58%

Transfection Efficiency: 90%

Primary

Mouse Cerebral Cortex Neurons

9 days after EP

Red: MAP2 Blue: GFAP Green: GFP The cells were from Embryonic Day 14 Mouse Cerebral Cortex

Viability 80%

Transfection Efficiency 70%

Primary

Mouse Neural Stem cells

Viability: Good

Transfection Efficiency: 37%

Primary

Mouse Microglia cells

Viability 65%

Transfection Efficiency 23%

Primary MEF

Mouse Embryonic Fibroblasts

Viability: 90%

Transfection Efficiency: 85%

Primary

Mouse Embryonic Skin Fibroblasts

Viability: 90%

Transfection Efficiency: 50%

Primary BMMC

Mouse Bone Marrow-Derived Mast Cells

Viability 64%

Transfection Efficiency 78%

Primary

Rat Bulbar Neurons

7 days after EP / The cells were from Embryonic Day 15 Rat Bubblar Neurons

Viability 80%

Transfection Efficiency 75%

Primary

Rat Meningeal Fibroblasts

Viability: 90%

Transfection Efficiency: 95%

Primary

Rat Meningeal Fibroblasts

Viability 90%

Transfection Efficiency 95%

Primary

Rat Meningeal Fibroblasts

Viability: 90%

Transfection Efficiency: 95%

Primary OEC

Rat Olfactory Ensheathing Cells

5 days after electroporation / The cells were from Postnatal Week 3 / Electroporation: after being cultured for 2 weeks.

Viability 93%

Transfection Efficiency 46%

Primary

Chick Embryonic Cerebellar Granule Cells

Viability:

Transfection Efficiency: 35%

3T3-L1 Mouse Preadipocytes

In Adherence

Viability: 90%

Transfection Efficiency: 70%

24 hours after electroporation

Electroporation: After 7 days of differentiation

SH-SY5Y

Human Neuroblastoma cells

In Adherence

Viability: 90%

Transfection Efficiency: 50%

C6 Rat Glioma cells
In Adherence

Viability: 80%

Transfection Efficiency: 39%

Primary Mouse Neurons
In Adherence

 Viability Good

Transfection Efficiency Good

Primary Mouse Neurons In Adherence

(High magnification)

Primary Mouse Neurons In Adherence

(High magnification)

Primary Mouse Hippocampal Neurons

In Adherence

Viability: 85%

Transfection Efficiency: 54%

Primary Mouse Glial cells

In Adherence

Viability: 80%

Transfection Efficiency: 50%