Cat Cloning – LF101/LF201

Device and Electrode Recommendation

Device Recommendation:

We recommend the following Electo Cell Fusion devices:

– LF101, and

– LF201

Electrode Recommendation:

We recommend the following electrodes with the LF101 or LF201 electro cell fusion devices:

Note:

¥ Since the size of the embryo for those kinds of animal models is about 100uM, the diameter of CUY5100-100 which is 100uM covers the entire embryo

π As one will observe, the suffix of the catalogue number indicates the diameter of the electrode. Accordingly, the most appropriate electrode is chosen by reference to the size of the embryo which one works

Protocol Recommendation:

Kindly note the following cloning publications:

– ‘In vitro development of cloned embryos derived from miniature pig somatic cells after activation by ultrasound stimulation’

– ‘Development of porcine embryos reconstituted with somatic cells and enucleated metaphase I and II oocytes matured in a protein free mediium’

– ‘Nuclear transfer of synchronized Africian wild cat somatic cells into enucleated domestic cat oocytes’