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SONIDEL SP100 and KTAC4000
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Featured Device

0
Product Code: SONIDEL SP100

Desc:Sonoporator
Applic:(hover for application)Application:
  • Delivery of plasmids to cells and tissues for gene therapy-based applications and studies.
  • Delivery of nucleic acids such as siRNA, RNAi etc. to cells and tissues for studies on control of gene expression/gene therapy.
  • Delivery of cancer chemotherapeutic agents to impermeable target cells/tissues.
  • Delivery of agents to cells to study metabolic effects.


Featured Electrode



Product Code: CUY500G1

Application:
Electrodes for Electro Cell Fusion
Description:
Micro Slide Gold Wire Electrode - 1.0mm gap, Volume 40l

Information
- How to order
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Cat Cloning – LF101/LF201

Device and Electrode Recommendation

Device Recommendation:

We recommend the following Electo Cell Fusion devices:

- LF101, and

- LF201

Electrode Recommendation:

We recommend the following electrodes with the LF101 or LF201 electro cell fusion devices:

Animal Model                         Note

Electrode Recommendation

Bovine                          ¥

Porcine                         ¥

Cat                              ¥

Dog                             ¥

Rabbit                          ¥

CUY5100-100

CUY5100-00

CUY5100-100

CUY5100-100

CUY5100-100

Mouse Embryo              π

Mouse embryo             n

Mouse Embryo             n

CUY5100-10

CUY5100-25

CUY5100-50

Note:

¥ Since the size of the embryo for those kinds of animal models is about 100uM, the diameter of CUY5100-100 which is 100uM covers the entire embryo

π As one will observe, the suffix of the catalogue number indicates the diameter of the electrode. Accordingly, the most appropriate electrode is chosen by reference to the size of the embryo which one works

Protocol Recommendation:

Kindly note the following cloning publications:

- ‘In vitro development of cloned embryos derived from miniature pig somatic cells after activation by ultrasound stimulation’

- ‘Development of porcine embryos reconstituted with somatic cells and enucleated metaphase I and II oocytes matured in a protein free mediium’

- ‘Nuclear transfer of synchronized Africian wild cat somatic cells into enucleated domestic cat oocytes’


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