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Top » Product Catalogue » Cell Fusion Generators » LF201 »

LF201 - Electro Cell Fusion Generator

Applications

  • ES/EG Cell and T-Cell (Activation or Reprogramming of
    Pluripotent Stem Cell)
  • Human Dendtritic and Tumor Cells (Cancer Vaccine)
  • Yeast and Mold
  • Islet Cells and Tumor Cells (Transplantation of Insulin-Secreting Cell)
  • Spleen and Myeloma cell fusion (ELISA)

LF 201

  • Plant Protoplast Fusion
  • Human Monoclonal Antibody production
    (Treatment of Breast Cancer with Anti-Cancer Drug)
  • Electrofusion of Recipient Oocyte and Somatic Cells
    (Bovine, Pig, Mouse and Rabbit Cloning)
  • Electrofusion of Hamster or Mouse 2-Cell Blastomeres
    (Tetraploid Embryo)



Cell Fusion

Nuclear Transfer (Cloning)

Oocyte Activation


Electro Cell Fusion Advantages v PEG


  • Greater control on the fusion
  • Double/triple efficiency compared with PEG
  • Electro cell fusion results are more stable
  • No Toxicity
  • Reproducibility not dependant on Researcher Skill

Features

 

Increased Reproducibility

To ensure accurate and reproducible cell fusion it is essential to align the
target cells prior to the fusion event. If the ion concentration of the buffer
is too high, ions will cause a water stream which will disturb the cell
alignment (pearl chain). Uniquely, the LF201 enables the researcher to
measure the resistance of the buffer prior to cell fusion and ensure the
correct ion concentration for exact cell alignment (in all experiments).
This greatly enhances reproducibility.

AC / DC Switching Time

The AC current is used to make a pearl chain (line of cells). For effective
cell fusion, it is imperative that the DC pulse is applied immediately after
the AC pulse and when the target cells are still actually in physical contact.
With the LF201, the switching time from AC pulse to DC pulse is
less than 5µsec.

Two Kinds of DC Pulse Outputs

The LF201 offers two possible DC pulse modes. A positive (+) square wave
or a bipolar (+/-) square wave. The first is a single direction pulse and in
the other the pole of the DC pulse is switched to the opposite polarity
during pulse delivery.

Post Fusion

There are two different types of post fusion AC current. One, a standard
Sine Wave (constant amplitude). The other, a Fade Wave (gradually
decreasing amplitude).

DC Pulse Output

The LF201 allows the researcher to output the DC pulse either
automatically or manually. The automatic output mode facilitates routine
work. The manual output mode enables the researcher to apply a DC
pulse while using a microscope - this is particularly useful when working
with unfamiliar cells and during nuclear transplantation.

PC Interfacing

The LF201 can be controlled via Microsoft Windows compatible software
where a cell fusion program can be entered and edited through a PC.
Each program can generate a text file capturing details such as experiment
time/date, resistance data etc..

Ease of Operation

For ease of operation, each cell fusion parameter is displayed on its own
individual LED.

Specifications

AC Pulse

Wave Shape


Voltage

Frequency

Duration

Post Fusion AC Mode

Shunt Resistor

Resistance Measurement

AC/DC Switching Time

Memory

  Sine Wave


0-75Vrms, 1V resolution

1MHz

Pre-Fusion: 0-100sec (1sec resolution)

Post-Fusion: 0-10sec (1sec resolution)

Sine or Fade Wave*

Greater than 50Ω
Up to 39.99kΩ

Less than 5µs

99 protocols

DC Pulse

Wave Shape


Voltage

Pulse Length

Pulse Interval

Number of Pulses

Output

Weight

Power

Dimensions

  Normal Mode: positive (+) Square Wave Pulse


Bipolarity Mode: Bipolar (+/-) Square Wave Pulse

0-1200V (1V resolution)

0-100µs (1µsec resolution)

0.1-10sec (0.1sec resolution)

1-100

Automatic or Manual

9kg

100V-240V

375W x 360D x 170H mm

* In normal mode, AC amplitude is constant. In fade-out mode, AC amplitude decreases.

Applications and Electrode Selection : Cell Fusion

Applications and Electrode Selection

Nuclear Transfer (Cloning) and Oocyte Activation

Nuclear Transfer

LF201

LF201 Specifications:

LF201 - Electro Cell Fusion Generator - for Cell Fusion

  

Applications

•ES/EG Cell and T-Cell (Activation of Reprogramming of Pluripotent Stem Cell)

•Human Dendtritic Cell and Tumor Cell (Cancer Vaccine) 

•Yeast and Mold

•Islet Cell and Tumor Cell (Transplantation of Insulin-Secreting Cell)

•Splenic Cell and Myeloma (ELISA) 

•Plant Protoplast Fusion

•Human Monoclonal Antibody (Treatment of Breast Cancer with Anti-Cancer Drug)

•Electrofusion of Recipient Oocyte and Somatic Cell (Bovine, Pig, Mouse and Rabbit Cloning)

•Electrofusion of Hamster or Mouse 2-Cell Blastomeres (Tetraploid Embryo)

 

 

 

Features

• Resistance Measurement Function

The resistance of the actual sample and buffer can be measured up to 39.99 k?.

 

• AC/DC Switching Time

Switching time from AC pulse to DC pulse is less than 5µs.

 

• Two Kinds of DC Pulse Outputs

DC square pulse can be chosen from either positive (+) or bipolar (+/-) pole.

 

Memory

Up to 99 protocols can be stored.

• Two Types of Post Fusion

Post fusion can be done in either Sine Wave or Fade Wave.

  

• DC Pulse Output

DC pulse can be output automatically or manually

 

• PC Control

The LF201 can be controlled via a PC. The software is compatible with Windows 2000 and Windows XP.

 

• Conversion

Protocol data can be converted into Excel format.

 

   Specifications

   AC

   DC Pulse

Wave Shape

Sine Wave

Wave Shape

Positive (+) Square Wave Pulse or Bipolar (+/-) Square Wave Pulse

Voltage

0-75Vrms, 1V resolution

Frequency

1MHz

Voltage

0-1,200V (1V resolution)

Duration

0-100s (1sec resolution)

Pulse Length

0-100µs (1µsec resolution)

Post Fusion AC

0-10s (Sine or Fade Wave)

Pulse Interval

0.1-10s (0.1sec resolution)

Shunt Resistor

Greater than 50?

Number of Pulses

1-100 (Automatic/Manual)

 

Resistance Measurement

Up to 39.99k?

System Requirement

Windows 2000 and Windows XP

AC/DC Switching Time

Less than 5µs

Power

100V-240V

Memory

99 protocols

Dimensions, Weight

375W x 360D x 170H mm, 9kg


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