Mouse Embryo – Eye

Electrode Recommendation:

With respect to gene delivery into Mouse Embryo’s Eye (and Brain) one can use the tungsten needle type electrode. However, as the mouse embryo is very small it is often hard to inject DNA into embryo’s eye.  Accordingly:

For gene delivery into Mouse Embryo - Retina we recommend:

-          CUY675P3 and

-          CUY675P5

For your information, some clients prefer to use the CUY650P7 electrode for gene delivery into mouse retina.

Technical Drawing: CUY650P7

(Dr. Matsuda uses the CUY650P7 electrode in his articles contained in the ‘EP with Retina’ below).

For gene delivery into Mouse Embryo - Cornea we recommend:

-          CUY671P1 or                                        Technical Drawing: CUY671P1

-          CUY670                                                  Technical Drawing: CUY670

Protocol Recommendation:

Download ‘EP with Retina’

EP with Retina PDF includes:

-  ‘Electroporation and RNA interference in the rodent retina in vivo and in vitro‘

-  ‘Protocol for in vivo electroporation into mouse/rat retina‘

-  ‘Compensation by tumor suppressor genes during retinal development in mice and humans‘

For your further information, note the article titled:

-  ‘A role for ligand-gated ion channels in rod photoreceptor development’.

In this article and ‘Electroporation and RNA interference in the rodent retina in vivo and in vitro’ above genes are transfected into mouse and rat retina.  We believe that the method outlined in these articles can be applied to Gene Therapy