Mouse Embryo – Entire Brain
Electrode Recommendation:
If one is specifically interested in mouse embryo (entire brain) - we recommend the CUY650P3 electrode for all stages earlier than E12.5 or the CUY650P5 electrode for all stages later than E12.5.
Technical Drawing: CUY650P Series
Recommended Protocol:
|
Stage |
Electrode Diameter |
Electrode |
Voltage |
Pulse Length |
Pulse Interval |
Cycles |
Current |
|
E12.5 |
3mm |
33V |
30 msec |
970 msec |
4 |
30-60mA |
|
|
E13.5 |
5mm |
30V |
50 msec |
950 msec |
4 |
30-60mA |
|
|
E14.5 |
5mm |
33V |
50 msec |
950 msec |
4 |
30-60mA |
|
|
E15 |
5mm |
35V |
50 msec |
950 msec |
4 |
30-60mA |
In this case, genes are introduced into the entire brain.