Electrode Recommendation:
With respect to in utero Electroporation, we recommend the following:
- CUY650P3 (If the mouse embryo is younger than E12.5)
- CUY650P5 (If the mouse embryo is older than E12.5)
Kindly note below for the Technical Drawings of these electrodes.
Protocol Recommendation:
Kindly note the following ‘In Utero Electroporation Resource’ file which includes the following articles:
- ‘Manipulating gene expressions by electroporation in the developing brain of mammalian embryos’
- ‘Multipolar Migration: The Third Mode of Radial Neuronal Migration in the Developing Cerebral Cortex’
- ‘Radial Migration of Superficial Layer Cortical Neurons Controlled by Novel Ig Cell Adhesion Molecule MDGA1′
- ‘Mammalian BarH1 Confers Commissural Neuron Identity on Dorsal Cells in the Spinal Cord’
- Technical Drawing of CUY651 electrode
- In utero Electroporation Parameters
- Technical Drawing of CUY650P1, P3 electrodes
- Technical Drawing of CUY650-5, P5, 7, P7 electrodes
- Photo of mouth-controlled micropipette system
Download: ‘In Utero Electroporation Resource’ file
Kindly also note the following in utero Brain Applications. Identify the Application of interest and then click on the relevant Protocol to download it.
Application
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Protocol
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CdK5 Kinase - Cortex (2003)
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Serine 732 Phosphorylation of FAK by Cdk5 Is Important for Microtubule Organization, Nuclear Movement, and Neuronal Migration’
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Central Nervous System (2006)
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In Vivo Electroporation in the Embryonic Mouse Central Nervous System
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Cerebral Cortical Nueron (2005)
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Birth-Date-Dependent Segreation of the Mouse Cerebral Cortical Neurons in Re-Aggregation Cultures
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Cerebral Cortical Progenitors (2005)
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G Protein By Subunits and AGS3 Control Spindle Orientation and Asymmetric Cell Fate of Cerebral Cortical Progenitors
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Cortex (2003)
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Multipolar Migration: The Third Mode of Radial Neuronal Migration in the Developing Cerebral Cortex
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Cortex - Brain (2001)
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Efficient In Utero Gene Transfer System to the Developing Mouse Brain using Electroporation: Visulization of Neuronal Migration in the Developing Cortex
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Cortex - Brain (2003)
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Emx2 Patterns the Neocortex by Regulating FGF Positional Signalling
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Cortical Neurons (2006)
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Radial Migration of Superficial Layer Cortical Neurons Controlled by Novel Ig Cell Adhesion Molecule MDGA1
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Glia Cortex - Brain (2003)
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Layering Defect in p35 Deficiency is linked to Improper Neuronal-Glial Interaction in Radial Migration
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Hes Gene - Telencephalon (2004)
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Hes genes regulate size, shape and histogenesis of the nervous system by control of the timing of neural stem cell differentiation
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Hippocampus (2005)
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Neuronal Generation, Migration, and Differentation in the Mouse Hippocampal Primoridium as Revealed by Enhanced Green Fluorescent Protein Gene Transfer by Means of In Utero Electroporation
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In Utero Electrodes
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Electroporation Protocol; Technical Drawing of CUY651, CUY650P1, P3, CUY650-5, P5, 7, P7 Electrodes and Mouth-controlled Micropipette System Photo
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JNK - Cerebral Cortex (2003)
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The In Vivo Roles of STEF/Tiam1, Rac1 and JNK in Cortical Neuronal Migration
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Neural Stem Cell - Brain (2001)
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Roles of the Basic Helix-Loop-Helix Genes Hes1 and Hes5 in Expansion of Neural Stem Cells of the Developing Brain*
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NUDEL - Brain (2004)
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A NUDEL - Dependent Mechanism of Neurofilament Assembly Regulates the Integrity of CNS Neurons
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Spinal Cord (2003)
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Mammalian BarH1 Confers Commissural Neuron Identity on Dorsal Cells in the Spinal Cord
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Spinal Cord (2005)
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Commissural Neuron Identity is Specified by a Homeodomain Protein, Mbh1, that is Directly Downstream of Math1
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Telencephalon (2004)
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Generation of Reelin-Positive Marginal Zone Cells from the Caudomedial Wall of Telencephalic Vesicles
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The application titled above as ‘In Utero Electrodes’ identifies appropriate protocols for in utero electroporation.
For in utero electroporation parameters with Adult Mice Brain; please note the following links.
- ‘Locus-Specific Rescue of GluR1€ NMDA Receptors in Mutant Mice Identifies the Brain Regions Important for Morphine Tolerance and Dependence’
Download Article
- ‘Multipolar Migration: The Third Mode of Radial Neuronal Migration in the Developing Cerebral Cortex’
Download Article
- ‘Shocking Development in Chick Embryology Electroporation and in ovo Gene Expression’
Download Article
- ‘Gene Transfer into Cultured Mammalian Embryos by Electroporation’
Download Article
Kindly also note the following link to Prof. Saito (Chiba University) explaining, in detail, best practise in utero electroporation procedure and protocol.
