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NEPA21 Electroporator
ELEPO21 Electroporator
NEPA Porator
ECFG Porator
Transgenic Mouse Zygote Electroporation: NEPA21
Organoid Electroporation: NEPA21
Neuroscience & Genome Editing Publications
Hybridoma Production for Monoclonal Antibodies
Bacteria / Yeast Electroporation
SP100: Sonoporator
NEPA21: Illustrated  Applications
NEPA21: Publications by   Research Application
- Connection Cables
- CU700: Monitoring System
- CU902: Polarity Exchanger
- CUY21 EDIT Electroporator
- CUY21 EDIT-S Electroporator
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- In Vivo, In vitro Electrodes
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- Cuvette Chamber & Stand   Holder
- Laser Thermal Microinjector
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- Ultrasounic BioMicroscope
- KTAC-4000: Sonoporator
- LF101: Cell Fusion Device
- LF201: Cell Fusion Device
- Electro Cell Fusion Electrodes
Electroporation and Electro   Cell Fusion Accessories
- Electroporators
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- Electrodes by Product Codes

 

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Featured Applications
Sonoporation
SONIDEL SP100 and KTAC4000
Electroporation
CUY21 EDIT, CUY21 SC
Electro Cell Fusion
LF101 and LF201


Featured Device

3
Product Code: OCH01

Desc:Cell Holder
Applic:Electroporation and Sonoporation


Featured Electrode



Product Code: CUY659P2-15

Application:
Electrodes for Electroporation
Description:
Tweezers with 2mm exposed needle (bent) & 2mmf platinum disk electrodes on tip, both tweezers are bent.

Information
- How to order
- Contact us

Cultured Mouse Embryos and Large Tissue

Cultured Mouse and Rat Embryo and Large Tissue EP

Whole Mammalian Embryo EP:
There is a choice of two different electrodes:
–    CUY520P20 and
–    CUY650P7
(Click on links to view a technical drawing of the electrodes or similar electrodes in the series)

Please note the following article which explains the appropriate Methodology:
–    Electroporation into Cultured Mammalian Embryos
–    Methodology Summarised

Applicable Stages for Whole-Embryo Culture

E8.5

9.5

10.5

11.5

12.5

13.5

14.5

15.5

Rat Stages

6.5

7.5

8.5

9.5

10.5

11.5

12.5

13.5

Mouse Stages

NOTES
The volume of a bath can be changed by placing a small bath made of agarose gel in the bath.
Please refer to the Application Note: ‘Method to introduce genes into epithelial cells of the chicken embryonic stomach (proventriculus)’.

Embryonic Tissues (Kidney and Lung)
Result form client lab. for Embryonic Tissues (Kidney and Lung) using our CUY520P5 electrode (technical drawing).

 NEPA21 – Ex Vivo Publications
Acute Inhibition of PI3K-PDK1-Akt Pathway Potentiates Insulin Secretion through Upregulation of Newcomer Granule Fusions in Pancreatic β-Cells
Cortical excitatory neurons become protected from cell division during neurogenesis in an Rb family-dependent manner
Electroporation-mediated gene transfer system applied to cultured CNS neurons
Imaging of Insulin Exocytosis from Pancreatic Beta Cells
– In vivo ex vivo retina – ‘Chapter 19:Analysis of Gene Function in the Reina
PSD95β regulates plasma membrane Ca(2+) pump localization at the photoreceptor synapse
The Caudal Migratory Stream, The Caudal Migratory Stream: A Novel Migratory Stream ofInterneurons Derived from the Caudal Ganglionic Eminence in the Developing Mouse Forebrain

 

Small Tissue:
Electrode Recommendations:
–           CUY700 series and
–           CUY701 series
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe that the electroporation methods described in:
–  Electroporation-mediated gene transfer system applied to cultured CNS neurons
–  Early subdivisions in the neural plate define distinct comptence for inductive signals
are appropriate, that is, placing an electrode on the top of a tissue which is on another electrode.  A number of electrode option recommendations are appropriate:

Electrode Option 1:

–           CUY700P2E and CUY700P1L or CUY195P0.5

Electrode Option 2:

–          CUY700P2E and CUY614 with CUY615, one CUY613P1 or CUY611P3-1

 

See also Mouse and Rat Tissue/Brain Slice Recommendations


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