Adherent Cells
Electrode Recommendation
Previously, when clients requested an electrode recommendation for a 35mm diameter dish we suggested the CUY510 electrode.
However, as a result of client feedback we now know that this type of electroporation can be performed (much more successfully) on the entire dish using the:
- CUY512-5
- CUY513-4 and
- CUY513-5 electrodes
Download Technical Drawing of CUY512 and CUY513 electrodes and Electric Field
Electroporation Parameters
| Model | Electrode |
Cell |
Voltage |
Pulse length |
Pulse interval |
No. of Pulses |
Optimal |
Note | |||||||
| CUY21EDIT | CUY513-5 |
Neuron Cell |
110V |
5msec |
99msec or 95msec |
5 pulses or 5 pulses, (reverse polarity) |
0.15A |
50-70% confluent Hiporation buffer is added. |
|||||||
| CUY21EDIT | CUY513-5 |
Hippocampal Neuron Cell |
110V |
2msec | 98msec | 5 pulses 5 pulses (reverse polarity) |
0.15A |
50-70% confluent | |||||||
| CUY21EDIT | CUY512-5 |
Fibroblast Cell |
300V |
5msec | 95msec | 3 pulses 3 pulses (reverse polarity) |
0.15A |
Efficiency of gene transfection : 30% Viability : 60% |
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| CUY21SC | CUY512-5 |
Muscle Cell |
80-90V |
10msec | 90msec | 10 pulses 10 pulses (reverse polarity) |
0.17A |
50-70% confluent When current < 170mA, efficiency of gene transfection is low. When current > 200mA, cells are peeled off. |
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| CUY21EDIT | CUY513-5 |
Hela Cell |
7-14V |
60ms | 50ms | 4 pulses 4 pulses (reverse polarity) |
N/A |
Peptide transfection | |||||||
Kindly note the following article:
- ‘Expression Regulation of Hyaluronan Synthase in Corneal Endothelial Cells’