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NEPA21 Electroporator
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0
Product Code: SONIDEL SP100

Desc:Sonoporator
Applic:(hover for application)Application:
  • Delivery of plasmids to cells and tissues for gene therapy-based applications and studies.
  • Delivery of nucleic acids such as siRNA, RNAi etc. to cells and tissues for studies on control of gene expression/gene therapy.
  • Delivery of cancer chemotherapeutic agents to impermeable target cells/tissues.
  • Delivery of agents to cells to study metabolic effects.


Featured Electrode



Product Code: CUY660N10

Application:
Electrodes for Electroporation
Description:
Replacement stainless steel needle for CUY660, 5/pkg

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Mouse/Rat – Muscle
Since no surgery is involved, the researcher can easily perform electroporation and do so consecutively in a short period of time.  In our opinion, this is the best method.  However, as the volume of muscle affects the resistance value, and thus, actual…
 
Zebrafish Retina Electrode Recommendation
For Larval Zebrafish Retina (which is quite small, 10microns) it may not be possible to inject DNA buffer.   Accordingly, we suggest that electroporation is performed after the larval zebrafish eye is taken out.  Once this is done and one intends…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
Zebrafish Retina
For Larval Zebrafish Retina (which is quite small, 10microns) it may not be possible to inject DNA buffer.   Accordingly, we suggest that electroporation is performed after the larval zebrafish eye is taken out.  Once this is done and one intends…
 
In Ovo Electroporation
Some clients, however, who have to contend with a large number of samples on a daily basis find it difficult to manipulate a pair of electrodes vertically.  Such clients prefer to move the pair of electrodes horizontally.  The CUY613 series was designed…
 
Mouse and Rat Small Tissue Electrode Recommendation and Protocol
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe…
 
Mouse and Rat Small Tissue Electrode Recommendation and Protocol
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe…
 
Mouse/Rat – Muscle
Since no surgery is involved, the researcher can easily perform electroporation and do so consecutively in a short period of time.  In our opinion, this is the best method.  However, as the volume of muscle affects the resistance value, and thus, actual…
 
Mouse/Rat – Muscle
Since no surgery is involved, the researcher can easily perform electroporation and do so consecutively in a short period of time.  In our opinion, this is the best method.  However, as the volume of muscle affects the resistance value, and thus, actual…
 
Mouse and Rat Tissue and Brain Slice
 
Mouse and Rate Tissue and Brain Slice
 
Cultured Mouse Embryos and Large Tissue
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe…
 
Technical Drawing CUY701P2E
 
Technical Drawing: CUY701 series
 
Adult Mouse and Rat (In vivo) - Liver
Zang  et al.Inhibition  of  nuclear  delivery  of  plasmid  DNA  and  transcription  by  interferon   γ: hurdles to be overcome for sustained gene  therapy.Gene  Ther. 2011  Sep;18(9):891-7. doi: 10.1038/gt.2011.35. Epub 2011  Mar 31…
 
Chick Embryo (New Culture) - Gastrulation, Neural Plate (HH Stage 3-7)
For Chick Embryo (new culture) - Gastrulation, Neural Plate (HH Stage 3-7) applications we recommend the following electrodes: CUY701 seris (pair)
 
Photo: CUY701P20E and L
 
Chick and Quail Embryo (in ovo) - Neural Tube, Mesencephalon, Diencephalon (HH* Stage 10
Please also note the following links and attached articles for further information on the NEPA21’s In Ovo capacity. (Please note where a reference is made in the resource material to the CUY21 systems (EDIT or SC), the NEPA21 replaces them and can…
 
Tissue Fragment (Ex Vivo) - Brain Slice
For Tissue Fragment (ex vivo) - Brain Slice applications we recommend the following electrode: CUY701 series
 
Photo: CUY701P20E, P20L
 
Photo: CUY701P20E, P20L
 
Tissue Fragment (Ex Vivo) - Tissue Slice
For Tissue Fragment (ex vivo) - Brain Slice applications we recommend the following electrode: CUY701 series
 
Tissue Fragment (Ex Vivo) - Skin Slice
For Tissue Fragment (ex vivo) - Brain Slice applications we recommend the following electrode: CUY701 series
 
CUY21SC - Square Wave Electroporator
Because in vivo impedance (electrical resistance) is generally less stable than in vitro impedance, an accurate measurement of the target sample resistance prior to the electroporation event is the most important critical success factor for accurate…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
NEPA21_Retina_EP
(B) The current was applied with the positive electrode contralateral to the injected eye. After prior injection of plasmid DNA into the subretinal space of the right eye, this arrangement electrophoresed the negatively-charged DNA toward the RPE layer…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
Electroporation Protocols - In Vitro, In Ovo, In Utero, In Vivo, Ex Vivo and New Culture
 
 
Electroporation Protocols - In Vitro, In Ovo, In Utero, In Vivo, Ex Vivo and New Culture
 
Electroporation Protocols - In Vitro, In Ovo, In Utero, In Vivo, Ex Vivo and New Culture
 
Electroporation Protocols - In Vitro, In Ovo, In Utero, In Vivo, Ex Vivo and New Culture
 
Electroporation Protocols - In Vitro, In Ovo, In Utero, In Vivo, Ex Vivo and New Culture
 
NEPA21_Retina_EP
(B) The current was applied with the positive electrode contralateral to the injected eye. After prior injection of plasmid DNA into the subretinal space of the right eye, this arrangement electrophoresed the negatively-charged DNA toward the RPE layer…
 
Electroporation Protocols - In Vitro, In Ovo, In Utero, In Vivo, Ex Vivo and New Culture
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
LTM-1000 : Laser Thermal Microinjector
Heat-induced expansion of liquid in a sealed capillary generates high pressure, which enables injection by capillaries with the tip diameter around 0.1μm (Knoblauch et al., Nature Biotech., 1999). As the heat source, we chose a laser beam which enables…
 
LTM-1000 : Laser Thermal Microinjector
Heat-induced expansion of liquid in a sealed capillary generates high pressure, which enables injection by capillaries with the tip diameter around 0.1μm (Knoblauch et al., Nature Biotech., 1999). As the heat source, we chose a laser beam which enables…
 
LTM-1000 : Laser Thermal Microinjector
Heat-induced expansion of liquid in a sealed capillary generates high pressure, which enables injection by capillaries with the tip diameter around 0.1μm (Knoblauch et al., Nature Biotech., 1999). As the heat source, we chose a laser beam which enables…
 
LTM-1000 : Laser Thermal Microinjector
Heat-induced expansion of liquid in a sealed capillary generates high pressure, which enables injection by capillaries with the tip diameter around 0.1μm (Knoblauch et al., Nature Biotech., 1999). As the heat source, we chose a laser beam which enables…
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
LTM-1000 : Laser Thermal Microinjector
Heat-induced expansion of liquid in a sealed capillary generates high pressure, which enables injection by capillaries with the tip diameter around 0.1μm (Knoblauch et al., Nature Biotech., 1999). As the heat source, we chose a laser beam which enables…
 
LTM-1000 : Laser Thermal Microinjector
Heat-induced expansion of liquid in a sealed capillary generates high pressure, which enables injection by capillaries with the tip diameter around 0.1μm (Knoblauch et al., Nature Biotech., 1999). As the heat source, we chose a laser beam which enables…
 
LTM-1000 : Laser Thermal Microinjector
Heat-induced expansion of liquid in a sealed capillary generates high pressure, which enables injection by capillaries with the tip diameter around 0.1μm (Knoblauch et al., Nature Biotech., 1999). As the heat source, we chose a laser beam which enables…
 
Mouse – Tissue/Brain Slice
 
Mouse – Tissue/Brain Slice
 
Method to introduce genes into epithelial cells of the chicken embryonic stomach (proventriculus)
4. Apply pulses of 30V for 15 times with pulse length of 50ms, and interval time of 75ms.  Remove agarose gel immediately and wash it in Tyrode's solution.  Then take out the tissues from the gel and wash them well in Tryode's solution.  The tissues…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21 Publications by Research Interest
 
CUY21 Publications by Research Interest
 
CUY21 Publications by Research Interest
 
CUY21 Publications by Research Interest
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The CUY21SC measures and displays the delivered current immediately after an EP event. This data is crucial to enable the researcher to verify (on the basis of V=IR) that the correct electroporation protocol has been delivered. As the CUY21SC can measure…
 
CUY21SC - Square Wave Electroporator
The resistance of an EP target varies according to physical experimental factors such as the volume of the sample, the buffer used and the distance between the electrodes. Fluctuations in resistance impact on delivered current values and this negatively…
 
Gene transfer into embryonic brains using in utero electroporation technique
A 2 cm midline incision is then made in the abdominal wall along the linea alba using a set of forceps and scissors. A piece of sterile gauze with a hole cut in the center is placed over the incision, and one uterine horn is drawn out through the…
 
Gene transfer into embryonic brains using in utero electroporation technique
 
Gene transfer into embryonic brains using in utero electroporation technique
 
Gene transfer into embryonic brains using in utero electroporation technique
 
Gene transfer into embryonic brains using in utero electroporation technique
 
Gene transfer into embryonic brains using in utero electroporation technique
 
Gene transfer into embryonic brains using in utero electroporation technique
A 2 cm midline incision is then made in the abdominal wall along the linea alba using a set of forceps and scissors. A piece of sterile gauze with a hole cut in the center is placed over the incision, and one uterine horn is drawn out through the…
 
Gene transfer into embryonic brains using in utero electroporation technique
 
Gene transfer into embryonic brains using in utero electroporation technique
 
Gene transfer into embryonic brains using in utero electroporation technique
 
Gene transfer into embryonic brains using in utero electroporation technique
 
Gene transfer into embryonic brains using in utero electroporation technique
A 2 cm midline incision is then made in the abdominal wall along the linea alba using a set of forceps and scissors. A piece of sterile gauze with a hole cut in the center is placed over the incision, and one uterine horn is drawn out through the…
 
Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect
Stereomicroscopic views of transfected testes charged with various voltages and observed under visible (A) or excitation (B) light after 5 wk. Voltage is indicated on each testis (A). Loss of testicular weight 5 wk after electric charge in 12-day-old…
 
Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect
Stereomicroscopic views of transfected testes charged with various voltages and observed under visible (A) or excitation (B) light after 5 wk. Voltage is indicated on each testis (A). Loss of testicular weight 5 wk after electric charge in 12-day-old…
 
Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect
Stereomicroscopic views of transfected testes charged with various voltages and observed under visible (A) or excitation (B) light after 5 wk. Voltage is indicated on each testis (A). Loss of testicular weight 5 wk after electric charge in 12-day-old…
 
Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect
Stereomicroscopic views of transfected testes charged with various voltages and observed under visible (A) or excitation (B) light after 5 wk. Voltage is indicated on each testis (A). Loss of testicular weight 5 wk after electric charge in 12-day-old…
 
Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect
Stereomicroscopic views of transfected testes charged with various voltages and observed under visible (A) or excitation (B) light after 5 wk. Voltage is indicated on each testis (A). Loss of testicular weight 5 wk after electric charge in 12-day-old…
 
Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect
Stereomicroscopic views of transfected testes charged with various voltages and observed under visible (A) or excitation (B) light after 5 wk. Voltage is indicated on each testis (A). Loss of testicular weight 5 wk after electric charge in 12-day-old…
 
Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect
Stereomicroscopic views of transfected testes charged with various voltages and observed under visible (A) or excitation (B) light after 5 wk. Voltage is indicated on each testis (A). Loss of testicular weight 5 wk after electric charge in 12-day-old…
 
Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect
Stereomicroscopic views of transfected testes charged with various voltages and observed under visible (A) or excitation (B) light after 5 wk. Voltage is indicated on each testis (A). Loss of testicular weight 5 wk after electric charge in 12-day-old…
 
Gene transfer into embryonic brains using in utero electroporation technique
 
Method to introduce genes into epithelial cells of the chicken embryonic stomach (proventriculus)
4. Apply pulses of 30V for 15 times with pulse length of 50ms, and interval time of 75ms.  Remove agarose gel immediately and wash it in Tyrode's solution.  Then take out the tissues from the gel and wash them well in Tryode's solution.  The tissues…
 
Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect
Stereomicroscopic views of transfected testes charged with various voltages and observed under visible (A) or excitation (B) light after 5 wk. Voltage is indicated on each testis (A). Loss of testicular weight 5 wk after electric charge in 12-day-old…
 
Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect
Stereomicroscopic views of transfected testes charged with various voltages and observed under visible (A) or excitation (B) light after 5 wk. Voltage is indicated on each testis (A). Loss of testicular weight 5 wk after electric charge in 12-day-old…
 
Gene transfer into embryonic brains using in utero electroporation technique
 
Gene transfer into embryonic brains using in utero electroporation technique
 
Gene transfer into embryonic brains using in utero electroporation technique
A 2 cm midline incision is then made in the abdominal wall along the linea alba using a set of forceps and scissors. A piece of sterile gauze with a hole cut in the center is placed over the incision, and one uterine horn is drawn out through the…
 
Method to introduce genes into epithelial cells of the chicken embryonic stomach (proventriculus)
4. Apply pulses of 30V for 15 times with pulse length of 50ms, and interval time of 75ms.  Remove agarose gel immediately and wash it in Tyrode's solution.  Then take out the tissues from the gel and wash them well in Tryode's solution.  The tissues…
 
Method to introduce genes into epithelial cells of the chicken embryonic stomach (proventriculus)
4. Apply pulses of 30V for 15 times with pulse length of 50ms, and interval time of 75ms.  Remove agarose gel immediately and wash it in Tyrode's solution.  Then take out the tissues from the gel and wash them well in Tryode's solution.  The tissues…
 
Method to introduce genes into epithelial cells of the chicken embryonic stomach (proventriculus)
4. Apply pulses of 30V for 15 times with pulse length of 50ms, and interval time of 75ms.  Remove agarose gel immediately and wash it in Tyrode's solution.  Then take out the tissues from the gel and wash them well in Tryode's solution.  The tissues…
 
Electroporation-mediated gene transfer in the adult rat brain
 
Method to introduce genes into epithelial cells of the chicken embryonic stomach (proventriculus)
4. Apply pulses of 30V for 15 times with pulse length of 50ms, and interval time of 75ms.  Remove agarose gel immediately and wash it in Tyrode's solution.  Then take out the tissues from the gel and wash them well in Tryode's solution.  The tissues…
 
Electroporation of Xenopus embryo: Gene Delivery into the Primordium Eye at the Neural Plate Stage
Fig.(1) shows the general setting of the electroporation. Vitelline membrane of the embryo (st.12-13) is usually kept intact. Inject 5-10nl of GFP-mRNA(1µg/µl, 0.05% Fast Green) into intercellular space of upper-few epithelial layers of the neural…
 
Electroporation of Xenopus embryo: Gene Delivery into the Primordium Eye at the Neural Plate Stage
Fig.(1) shows the general setting of the electroporation. Vitelline membrane of the embryo (st.12-13) is usually kept intact. Inject 5-10nl of GFP-mRNA(1µg/µl, 0.05% Fast Green) into intercellular space of upper-few epithelial layers of the neural…
 
Electroporation of Xenopus embryo: Gene Delivery into the Primordium Eye at the Neural Plate Stage
Fig.(1) shows the general setting of the electroporation. Vitelline membrane of the embryo (st.12-13) is usually kept intact. Inject 5-10nl of GFP-mRNA(1µg/µl, 0.05% Fast Green) into intercellular space of upper-few epithelial layers of the neural…
 
Electroporation of Xenopus embryo: Gene Delivery into the Primordium Eye at the Neural Plate Stage
Fig.(1) shows the general setting of the electroporation. Vitelline membrane of the embryo (st.12-13) is usually kept intact. Inject 5-10nl of GFP-mRNA(1µg/µl, 0.05% Fast Green) into intercellular space of upper-few epithelial layers of the neural…
 
Electroporation of Xenopus embryo: Gene Delivery into the Primordium Eye at the Neural Plate Stage
Fig.(1) shows the general setting of the electroporation. Vitelline membrane of the embryo (st.12-13) is usually kept intact. Inject 5-10nl of GFP-mRNA(1µg/µl, 0.05% Fast Green) into intercellular space of upper-few epithelial layers of the neural…
 
Electroporation of Xenopus embryo: Gene Delivery into the Primordium Eye at the Neural Plate Stage
Fig.(1) shows the general setting of the electroporation. Vitelline membrane of the embryo (st.12-13) is usually kept intact. Inject 5-10nl of GFP-mRNA(1µg/µl, 0.05% Fast Green) into intercellular space of upper-few epithelial layers of the neural…
 
Protocol for in vivo electroporation into mouse and rat retina
 
Protocol for in vivo electroporation into mouse and rat retina
 
Protocol for in vivo electroporation into mouse and rat retina
 
Protocol for in vivo electroporation into mouse and rat retina
 
Protocol for in vivo electroporation into mouse and rat retina
 
Protocol for in vivo electroporation into mouse and rat retina
 
Protocol for in vivo electroporation into mouse and rat retina
 
Protocol for in vivo electroporation into mouse and rat retina
 
Protocol for in vivo electroporation into mouse and rat retina
 
Epidermis-Targeted Gene Transfer Using In Vivo Electroporation
 
Epidermis-Targeted Gene Transfer Using In Vivo Electroporation
 
Epidermis-Targeted Gene Transfer Using In Vivo Electroporation
 
Electroporation-mediated gene transfer system applied to cultured CNS neurons
(d, e) A mature hippocampal neuron maintained 14 days in dissociated culture after electroporation of a ß-actin-eGFP expression construct. Higher magni¢cation view of the region marked by a rectangle in (d) reveals dendritic spines on the surface of…
 
Electroporation-mediated gene transfer system applied to cultured CNS neurons
(d, e) A mature hippocampal neuron maintained 14 days in dissociated culture after electroporation of a ß-actin-eGFP expression construct. Higher magni¢cation view of the region marked by a rectangle in (d) reveals dendritic spines on the surface of…
 
Electroporation-mediated gene transfer system applied to cultured CNS neurons
(d, e) A mature hippocampal neuron maintained 14 days in dissociated culture after electroporation of a ß-actin-eGFP expression construct. Higher magni¢cation view of the region marked by a rectangle in (d) reveals dendritic spines on the surface of…
 
Electroporation-mediated gene transfer system applied to cultured CNS neurons
(d, e) A mature hippocampal neuron maintained 14 days in dissociated culture after electroporation of a ß-actin-eGFP expression construct. Higher magni¢cation view of the region marked by a rectangle in (d) reveals dendritic spines on the surface of…
 
Electroporation-mediated gene transfer system applied to cultured CNS neurons
(d, e) A mature hippocampal neuron maintained 14 days in dissociated culture after electroporation of a ß-actin-eGFP expression construct. Higher magni¢cation view of the region marked by a rectangle in (d) reveals dendritic spines on the surface of…
 
Gene transfer into single cell by electroporation
 
Gene transfer into single cell by electroporation
 
Gene transfer into single cell by electroporation
 
Gene transfer into single cell by electroporation
 
Gene transfer into single cell by electroporation
 
Gene transfer into single cell by electroporation
 
Gene transfer into single cell by electroporation
 
Gene transfer into single cell by electroporation
 
Gene transfer into single cell by electroporation
 
Gene transfer into single cell by electroporation
 
Electroporation-mediated gene transfer in the adult rat brain
 
Electroporation-mediated gene transfer in the adult rat brain
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Method to introduce genes into epithelial cells of the chicken embryonic stomach (proventriculus)
4. Apply pulses of 30V for 15 times with pulse length of 50ms, and interval time of 75ms.  Remove agarose gel immediately and wash it in Tyrode's solution.  Then take out the tissues from the gel and wash them well in Tryode's solution.  The tissues…
 
Electroporation-mediated gene transfer in the adult rat brain
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Electroporation for mammalian embryos in the whole embryo culture system
(B-E) Time-lapse analysis of neuroepithelial cells in the slice culture system.  Histon-EGFP- and DsRed2-expression vectors were co-electroporated into the E12.0 rat spinal cord. The electroporated embryo was cultured for 24 hours in the WEC, then the…
 
Electroporation for mammalian embryos in the whole embryo culture system
(B-E) Time-lapse analysis of neuroepithelial cells in the slice culture system.  Histon-EGFP- and DsRed2-expression vectors were co-electroporated into the E12.0 rat spinal cord. The electroporated embryo was cultured for 24 hours in the WEC, then the…
 
Electroporation for mammalian embryos in the whole embryo culture system
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Misexpression of the gene of interest by in ovo electroporation
Mechanisms of brain regionalization and neural circuit formation have been studied by misexpression of transcription factors (En1/2, Pax2/5/6, Otx2, Gbx2), secreted factors (Fgf, Shh, semaphoring), signal transduction molecule (Ras, Sprouty2) and receptors…
 
Electroporation for mammalian embryos in the whole embryo culture system
(A) EGFP-expression vector was electroporated into E11.5 rat telencephalon. The electroporated embryo was cultured in the whole embryo culture system (WEC). (B) 24 hours after electroporation, EGFP-expression was specifically detected at the dorsal part…
 
Electroporation for mammalian embryos in the whole embryo culture system
(A) EGFP-expression vector was electroporated into E11.5 rat telencephalon. The electroporated embryo was cultured in the whole embryo culture system (WEC). (B) 24 hours after electroporation, EGFP-expression was specifically detected at the dorsal part…
 
Misexpression of the gene of interest by in ovo electroporation
Mechanisms of brain regionalization and neural circuit formation have been studied by misexpression of transcription factors (En1/2, Pax2/5/6, Otx2, Gbx2), secreted factors (Fgf, Shh, semaphoring), signal transduction molecule (Ras, Sprouty2) and receptors…
 
Misexpression of the gene of interest by in ovo electroporation
Mechanisms of brain regionalization and neural circuit formation have been studied by misexpression of transcription factors (En1/2, Pax2/5/6, Otx2, Gbx2), secreted factors (Fgf, Shh, semaphoring), signal transduction molecule (Ras, Sprouty2) and receptors…
 
Knock-down by transfection of shRNA expression vector by electroporation
(B) Select target DNA sequence of 19 to 21 mer. Sense and antisense sequence were linked to a nucleotide spacer as a loop and put into expression vector that is driven by U6 or H1 promoter. Commercially available expression vector that dreives expression…
 
Knock-down by transfection of shRNA expression vector by electroporation
(B) Select target DNA sequence of 19 to 21 mer. Sense and antisense sequence were linked to a nucleotide spacer as a loop and put into expression vector that is driven by U6 or H1 promoter. Commercially available expression vector that dreives expression…
 
Knock-down by transfection of shRNA expression vector by electroporation
(B) Select target DNA sequence of 19 to 21 mer. Sense and antisense sequence were linked to a nucleotide spacer as a loop and put into expression vector that is driven by U6 or H1 promoter. Commercially available expression vector that dreives expression…
 
Knock-down by transfection of shRNA expression vector by electroporation
(B) Select target DNA sequence of 19 to 21 mer. Sense and antisense sequence were linked to a nucleotide spacer as a loop and put into expression vector that is driven by U6 or H1 promoter. Commercially available expression vector that dreives expression…
 
Knock-down by transfection of shRNA expression vector by electroporation
(B) Select target DNA sequence of 19 to 21 mer. Sense and antisense sequence were linked to a nucleotide spacer as a loop and put into expression vector that is driven by U6 or H1 promoter. Commercially available expression vector that dreives expression…
 
Gene transfer into muscle by In Vivo electroporation
Electric pulses were delivered using an electric pulse generator (Square Wave Electroporator CUY21EDIT; Nepa Gene Co., Ltd.). Electrodes consisted of a pair of stainless steel needles of 5 mm in length and 0.4 mm in diameter, fixed with a distance (…
 
Gene transfer into muscle by In Vivo electroporation
Electric pulses were delivered using an electric pulse generator (Square Wave Electroporator CUY21EDIT; Nepa Gene Co., Ltd.). Electrodes consisted of a pair of stainless steel needles of 5 mm in length and 0.4 mm in diameter, fixed with a distance (…
 
Gene transfer into muscle by In Vivo electroporation
Electric pulses were delivered using an electric pulse generator (Square Wave Electroporator CUY21EDIT; Nepa Gene Co., Ltd.). Electrodes consisted of a pair of stainless steel needles of 5 mm in length and 0.4 mm in diameter, fixed with a distance (…
 
Gene transfer into muscle by In Vivo electroporation
Electric pulses were delivered using an electric pulse generator (Square Wave Electroporator CUY21EDIT; Nepa Gene Co., Ltd.). Electrodes consisted of a pair of stainless steel needles of 5 mm in length and 0.4 mm in diameter, fixed with a distance (…
 
Gene transfer into muscle by In Vivo electroporation
Electric pulses were delivered using an electric pulse generator (Square Wave Electroporator CUY21EDIT; Nepa Gene Co., Ltd.). Electrodes consisted of a pair of stainless steel needles of 5 mm in length and 0.4 mm in diameter, fixed with a distance (…
 
Chick embryo Electroporation using News culture gastrula
GFP gene was introduced to the prospective neural plate at HH4, and the embryo was cultured for about 34 hours (HH17 equivalent). GFP expression was occasionally monitored under a fluorescent dissecting microscope. GFP fluorescence was detectable as…
 
Chick embryo Electroporation using News culture gastrula
GFP gene was introduced to the prospective neural plate at HH4, and the embryo was cultured for about 34 hours (HH17 equivalent). GFP expression was occasionally monitored under a fluorescent dissecting microscope. GFP fluorescence was detectable as…
 
Chick embryo Electroporation using News culture gastrula
GFP gene was introduced to the prospective neural plate at HH4, and the embryo was cultured for about 34 hours (HH17 equivalent). GFP expression was occasionally monitored under a fluorescent dissecting microscope. GFP fluorescence was detectable as…
 
Chick embryo Electroporation using News culture (gastrula)
GFP gene was introduced to the prospective neural plate at HH4, and the embryo was cultured for about 34 hours (HH17 equivalent). GFP expression was occasionally monitored under a fluorescent dissecting microscope. GFP fluorescence was detectable as…
 
Chick embryo Electroporation using News culture (gastrula)
GFP gene was introduced to the prospective neural plate at HH4, and the embryo was cultured for about 34 hours (HH17 equivalent). GFP expression was occasionally monitored under a fluorescent dissecting microscope. GFP fluorescence was detectable as…
 
Chick embryo Electroporation using News culture gastrula
GFP gene was introduced to the prospective neural plate at HH4, and the embryo was cultured for about 34 hours (HH17 equivalent). GFP expression was occasionally monitored under a fluorescent dissecting microscope. GFP fluorescence was detectable as…
 
Chick embryo Electroporation using News culture gastrula
GFP gene was introduced to the prospective neural plate at HH4, and the embryo was cultured for about 34 hours (HH17 equivalent). GFP expression was occasionally monitored under a fluorescent dissecting microscope. GFP fluorescence was detectable as…
 
Chick embryo Electroporation using News culture gastrula
GFP gene was introduced to the prospective neural plate at HH4, and the embryo was cultured for about 34 hours (HH17 equivalent). GFP expression was occasionally monitored under a fluorescent dissecting microscope. GFP fluorescence was detectable as…
 
Chick embryo Electroporation using News culture gastrula
GFP gene was introduced to the prospective neural plate at HH4, and the embryo was cultured for about 34 hours (HH17 equivalent). GFP expression was occasionally monitored under a fluorescent dissecting microscope. GFP fluorescence was detectable as…
 
Direct Gene Transfer into Mature Seeds via Electroporation
Fertile transgenic plants were regenerated and self-fertilized seeds were obtained in rice and wheat. Transgene integration was confirmed by Southern hybridization. Transmission of the transgene into the next generation (T1) was indicated by PCR analysis…
 
Direct Gene Transfer into Mature Seeds via Electroporation
Fertile transgenic plants were regenerated and self-fertilized seeds were obtained in rice and wheat. Transgene integration was confirmed by Southern hybridization. Transmission of the transgene into the next generation (T1) was indicated by PCR analysis…
 
Direct Gene Transfer into Mature Seeds via Electroporation
Fertile transgenic plants were regenerated and self-fertilized seeds were obtained in rice and wheat. Transgene integration was confirmed by Southern hybridization. Transmission of the transgene into the next generation (T1) was indicated by PCR analysis…
 
Electrochemotherapy for digital chondrosarcoma
 
Electrochemotherapy for digital chondrosarcoma
 
Electrochemotherapy for digital chondrosarcoma
 
Electrochemotherapy for digital chondrosarcoma
 
Electrochemotherapy for digital chondrosarcoma
 
Electrochemotherapy for digital chondrosarcoma
 
Direct Gene Transfer into Mature Seeds via Electroporation
Fertile transgenic plants were regenerated and self-fertilized seeds were obtained in rice and wheat. Transgene integration was confirmed by Southern hybridization. Transmission of the transgene into the next generation (T1) was indicated by PCR analysis…
 
Direct Gene Transfer into Mature Seeds via Electroporation
Fertile transgenic plants were regenerated and self-fertilized seeds were obtained in rice and wheat. Transgene integration was confirmed by Southern hybridization. Transmission of the transgene into the next generation (T1) was indicated by PCR analysis…
 
Direct Gene Transfer into Mature Seeds via Electroporation
Fertile transgenic plants were regenerated and self-fertilized seeds were obtained in rice and wheat. Transgene integration was confirmed by Southern hybridization. Transmission of the transgene into the next generation (T1) was indicated by PCR analysis…
 
Direct Gene Transfer into Mature Seeds via Electroporation
Fertile transgenic plants were regenerated and self-fertilized seeds were obtained in rice and wheat. Transgene integration was confirmed by Southern hybridization. Transmission of the transgene into the next generation (T1) was indicated by PCR analysis…
 
Direct Gene Transfer into Mature Seeds via Electroporation
Fertile transgenic plants were regenerated and self-fertilized seeds were obtained in rice and wheat. Transgene integration was confirmed by Southern hybridization. Transmission of the transgene into the next generation (T1) was indicated by PCR analysis…
 
Direct Gene Transfer into Mature Seeds via Electroporation
Fertile transgenic plants were regenerated and self-fertilized seeds were obtained in rice and wheat. Transgene integration was confirmed by Southern hybridization. Transmission of the transgene into the next generation (T1) was indicated by PCR analysis…
 
Direct Gene Transfer into Mature Seeds via Electroporation
Fertile transgenic plants were regenerated and self-fertilized seeds were obtained in rice and wheat. Transgene integration was confirmed by Southern hybridization. Transmission of the transgene into the next generation (T1) was indicated by PCR analysis…
 
In vivo gene transfer into the adult honeybee brain by using electroporation
C) Schematic representation of the micropipette and electrodes placed in the honeybee brain. Purple indicates micropipette filled with DNA solution, blue indicates electrodes. AN, antennae; C, compound eyes; MB, mushroom bodies; Oc, ocelli; OL, optic…
 
In vivo gene transfer into the adult honeybee brain by using electroporation
C) Schematic representation of the micropipette and electrodes placed in the honeybee brain. Purple indicates micropipette filled with DNA solution, blue indicates electrodes. AN, antennae; C, compound eyes; MB, mushroom bodies; Oc, ocelli; OL, optic…
 
Direct Gene Transfer into Mature Seeds via Electroporation
Fertile transgenic plants were regenerated and self-fertilized seeds were obtained in rice and wheat. Transgene integration was confirmed by Southern hybridization. Transmission of the transgene into the next generation (T1) was indicated by PCR analysis…
 
Direct Gene Transfer into Mature Seeds via Electroporation
Fertile transgenic plants were regenerated and self-fertilized seeds were obtained in rice and wheat. Transgene integration was confirmed by Southern hybridization. Transmission of the transgene into the next generation (T1) was indicated by PCR analysis…
 
Direct Gene Transfer into Mature Seeds via Electroporation
Fertile transgenic plants were regenerated and self-fertilized seeds were obtained in rice and wheat. Transgene integration was confirmed by Southern hybridization. Transmission of the transgene into the next generation (T1) was indicated by PCR analysis…
 
Direct Gene Transfer into Mature Seeds via Electroporation
Fertile transgenic plants were regenerated and self-fertilized seeds were obtained in rice and wheat. Transgene integration was confirmed by Southern hybridization. Transmission of the transgene into the next generation (T1) was indicated by PCR analysis…
 
In vivo gene transfer into the adult honeybee brain by using electroporation
a) Schematic representation of the micropipette and electrodes placed in the honeybee brain. Purple indicates micropipette filled with DNA solution, blue indicates electrodes. AN, antennae; C, compound eyes; MB, mushroom bodies; Oc, ocelli; OL, optic…
 
In vivo gene transfer into the adult honeybee brain by using electroporation
a) Schematic representation of the micropipette and electrodes placed in the honeybee brain. Purple indicates micropipette filled with DNA solution, blue indicates electrodes. AN, antennae; C, compound eyes; MB, mushroom bodies; Oc, ocelli; OL, optic…
 
In vivo gene transfer into the adult honeybee brain by using electroporation
a) Schematic representation of the micropipette and electrodes placed in the honeybee brain. Purple indicates micropipette filled with DNA solution, blue indicates electrodes. AN, antennae; C, compound eyes; MB, mushroom bodies; Oc, ocelli; OL, optic…
 
Mouse and Rat Small Tissue Electrode Recommendation and Protocol
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe…
 
Mouse and Rat Small Tissue Electrode Recommendation and Protocol
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe…
 
Mouse and Rat Small Tissue Electrode Recommendation and Protocol
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe…
 
Mouse and Rat Small Tissue Electrode Recommendation and Protocol
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe…
 
Small Tissue
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe…
 
Mouse and Rat Small Tissue Electrode Recommendation and Protocol
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe…
 
Mouse and Rat Small Tissue Electrode Recommendation and Protocol
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe…
 
Mouse and Rat Tissue and Brain Slice
 
Mouse – Tissue/Brain Slice
 
Mouse and Rate Tissue and Brain Slice
 
Mouse and Rate Tissue and Brain Slice
 
Mouse and Rat Small Tissue Electrode Recommendation and Protocol
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe…
 
Zebrafish Retina Electrode Recommendation
For Larval Zebrafish Retina (which is quite small, 10microns) it may not be possible to inject DNA buffer.   Accordingly, we suggest that electroporation is performed after the larval zebrafish eye is taken out.  Once this is done and one intends…
 
Zebrafish Retina Electrode Recommendation
For Larval Zebrafish Retina (which is quite small, 10microns) it may not be possible to inject DNA buffer.   Accordingly, we suggest that electroporation is performed after the larval zebrafish eye is taken out.  Once this is done and one intends…
 
Mouse and Rate Tissue and Brain Slice
 
Zebrafish Retina Electrode Recommendation
For Larval Zebrafish Retina (which is quite small, 10microns) it may not be possible to inject DNA buffer.   Accordingly, we suggest that electroporation is performed after the larval zebrafish eye is taken out.  Once this is done and one intends…
 
CUY21SC - Square Wave Electroporator
Because in vivo impedance (electrical resistance) is generally less stable than in vitro impedance, an accurate measurement of the target sample resistance prior to the electroporation event is the most important critical success factor for accurate…
 
CUY21SC - Square Wave Electroporator
The resistance of an EP target varies according to physical experimental factors such as the volume of the sample, the buffer used and the distance between the electrodes. Fluctuations in resistance impact on delivered current values and this negatively…
 
CUY21SC - Square Wave Electroporator
Because in vivo impedance (electrical resistance) is generally less stable than in vitro impedance, an accurate measurement of the target sample resistance prior to the electroporation event is the most important critical success factor for accurate…
 
CUY21SC - Square Wave Electroporator
Because in vivo impedance (electrical resistance) is generally less stable than in vitro impedance, an accurate measurement of the target sample resistance prior to the electroporation event is the most important critical success factor for accurate…
 
CUY21SC - Square Wave Electroporator
Because in vivo impedance (electrical resistance) is generally less stable than in vitro impedance, an accurate measurement of the target sample resistance prior to the electroporation event is the most important critical success factor for accurate…
 
CUY21SC - Square Wave Electroporator
Because in vivo impedance (electrical resistance) is generally less stable than in vitro impedance, an accurate measurement of the target sample resistance prior to the electroporation event is the most important critical success factor for accurate…
 
CUY21SC - Square Wave Electroporator
Because in vivo impedance (electrical resistance) is generally less stable than in vitro impedance, an accurate measurement of the target sample resistance prior to the electroporation event is the most important critical success factor for accurate…
 
CUY21SC - Square Wave Electroporator
Because in vivo impedance (electrical resistance) is generally less stable than in vitro impedance, an accurate measurement of the target sample resistance prior to the electroporation event is the most important critical success factor for accurate…
 
CUY21SC - Square Wave Electroporator
Because in vivo impedance (electrical resistance) is generally less stable than in vitro impedance, an accurate measurement of the target sample resistance prior to the electroporation event is the most important critical success factor for accurate…
 
CUY21SC - Square Wave Electroporator
Because in vivo impedance (electrical resistance) is generally less stable than in vitro impedance, an accurate measurement of the target sample resistance prior to the electroporation event is the most important critical success factor for accurate…
 
CUY21SC - Square Wave Electroporator
Because in vivo impedance (electrical resistance) is generally less stable than in vitro impedance, an accurate measurement of the target sample resistance prior to the electroporation event is the most important critical success factor for accurate…
 
CUY21SC - Square Wave Electroporator
Because in vivo impedance (electrical resistance) is generally less stable than in vitro impedance, an accurate measurement of the target sample resistance prior to the electroporation event is the most important critical success factor for accurate…
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
CUY21SC - Square Wave Electroporator
Because in vivo impedance (electrical resistance) is generally less stable than in vitro impedance, an accurate measurement of the target sample resistance prior to the electroporation event is the most important critical success factor for accurate…
 
CUY21SC - Square Wave Electroporator
Because in vivo impedance (electrical resistance) is generally less stable than in vitro impedance, an accurate measurement of the target sample resistance prior to the electroporation event is the most important critical success factor for accurate…
 
New Electroporator
This page is password protected and is provided exclusively for the use of SONIDEL Limited customers who have signed up for membership of the SONIDEL Limited webstie (www.sonidel.com) AND who have specifically requested access to this information…
 
CUY21 Publication List, Electrode Recommendations and Protocol Information
 
New Electroporator
This page is password protected and is provided exclusively for the use of SONIDEL Limited customers who have signed up for membership of the SONIDEL Limited webstie (www.sonidel.com) AND who have specifically requested access to this information…
 
New Electroporator
This page is password protected and is provided exclusively for the use of SONIDEL Limited customers who have signed up for membership of the SONIDEL Limited webstie (www.sonidel.com) AND who have specifically requested access to this information…
 
New Electroporator
This page is password protected and is provided exclusively for the use of SONIDEL Limited customers who have signed up for membership of the SONIDEL Limited webstie (www.sonidel.com) AND who have specifically requested access to this information…
 
New Electroporator
This page is password protected and is provided exclusively for the use of SONIDEL Limited customers who have signed up for membership of the SONIDEL Limited webstie (www.sonidel.com) AND who have specifically requested access to this information…
 
New Electroporator
This page is password protected and is provided exclusively for the use of SONIDEL Limited customers who have signed up for membership of the SONIDEL Limited webstie (www.sonidel.com) AND who have specifically requested access to this information…
 
New Electroporator
This page is password protected and is provided exclusively for the use of SONIDEL Limited customers who have signed up for membership of the SONIDEL Limited webstie (www.sonidel.com) AND who have specifically requested access to this information…
 
New Electroporator
This page is password protected and is provided exclusively for the use of SONIDEL Limited customers who have signed up for membership of the SONIDEL Limited webstie (www.sonidel.com) AND who have specifically requested access to this information…
 
New Electroporator
This page is password protected and is provided exclusively for the use of SONIDEL Limited customers who have signed up for membership of the SONIDEL Limited webstie (www.sonidel.com) AND who have specifically requested access to this information…
 
New Electroporator
This page is password protected and is provided exclusively for the use of SONIDEL Limited customers who have signed up for membership of the SONIDEL Limited webstie (www.sonidel.com) AND who have specifically requested access to this information…
 
New Electroporator
This page is password protected and is provided exclusively for the use of SONIDEL Limited customers who have signed up for membership of the SONIDEL Limited webstie (www.sonidel.com) AND who have specifically requested access to this information…
 
New Electroporator
This page is password protected and is provided exclusively for the use of SONIDEL Limited customers who have signed up for membership of the SONIDEL Limited webstie (www.sonidel.com) AND who have specifically requested access to this information…
 
New Electroporator
This page is password protected and is provided exclusively for the use of SONIDEL Limited customers who have signed up for membership of the SONIDEL Limited webstie (www.sonidel.com) AND who have specifically requested access to this information…
 
New Electroporator
This page is password protected and is provided exclusively for the use of SONIDEL Limited customers who have signed up for membership of the SONIDEL Limited webstie (www.sonidel.com) AND who have specifically requested access to this information…
 
New Electroporator
This page is password protected and is provided exclusively for the use of SONIDEL Limited customers who have signed up for membership of the SONIDEL Limited webstie (www.sonidel.com) AND who have specifically requested access to this information…
 
New Electroporator
This page is password protected and is provided exclusively for the use of SONIDEL Limited customers who have signed up for membership of the SONIDEL Limited webstie (www.sonidel.com) AND who have specifically requested access to this information…
 
New Electroporator
This page is password protected and is provided exclusively for the use of SONIDEL Limited customers who have signed up for membership of the SONIDEL Limited webstie (www.sonidel.com) AND who have specifically requested access to this information…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
Electroporation-mediated gene transfer in the adult rat brain
 
Gene transfer into embryonic brains using in utero electroporation technique
A 2 cm midline incision is then made in the abdominal wall along the linea alba using a set of forceps and scissors. A piece of sterile gauze with a hole cut in the center is placed over the incision, and one uterine horn is drawn out through the…
 
Electroporation for mammalian embryos in the whole embryo culture system
(A) EGFP-expression vector was electroporated into E11.5 rat telencephalon. The electroporated embryo was cultured in the whole embryo culture system (WEC). (B) 24 hours after electroporation, EGFP-expression was specifically detected at the dorsal part…
 
Electroporation for mammalian embryos in the whole embryo culture system
(B-E) Time-lapse analysis of neuroepithelial cells in the slice culture system.  Histon-EGFP- and DsRed2-expression vectors were co-electroporated into the E12.0 rat spinal cord. The electroporated embryo was cultured for 24 hours in the WEC, then the…
 
Electroporation for mammalian embryos in the whole embryo culture system
(B-E) Time-lapse analysis of neuroepithelial cells in the slice culture system.  Histon-EGFP- and DsRed2-expression vectors were co-electroporated into the E12.0 rat spinal cord. The electroporated embryo was cultured for 24 hours in the WEC, then the…
 
Electroporation for mammalian embryos in the whole embryo culture system
(B-E) Time-lapse analysis of neuroepithelial cells in the slice culture system.  Histon-EGFP- and DsRed2-expression vectors were co-electroporated into the E12.0 rat spinal cord. The electroporated embryo was cultured for 24 hours in the WEC, then the…
 
Electroporation for mammalian embryos in the whole embryo culture system
(B-E) Time-lapse analysis of neuroepithelial cells in the slice culture system.  Histon-EGFP- and DsRed2-expression vectors were co-electroporated into the E12.0 rat spinal cord. The electroporated embryo was cultured for 24 hours in the WEC, then the…
 
Electroporation for mammalian embryos in the whole embryo culture system
(B-E) Time-lapse analysis of neuroepithelial cells in the slice culture system.  Histon-EGFP- and DsRed2-expression vectors were co-electroporated into the E12.0 rat spinal cord. The electroporated embryo was cultured for 24 hours in the WEC, then the…
 
Electroporation-mediated gene transfer system applied to cultured CNS neurons
(d, e) A mature hippocampal neuron maintained 14 days in dissociated culture after electroporation of a ß-actin-eGFP expression construct. Higher magni¢cation view of the region marked by a rectangle in (d) reveals dendritic spines on the surface of…
 
Electroporation-mediated gene transfer system applied to cultured CNS neurons
(d, e) A mature hippocampal neuron maintained 14 days in dissociated culture after electroporation of a ß-actin-eGFP expression construct. Higher magni¢cation view of the region marked by a rectangle in (d) reveals dendritic spines on the surface of…
 
Electroporation-mediated gene transfer in the adult rat brain
 
Electroporation-mediated gene transfer in the adult rat brain
 
Gene transfer into muscle by In Vivo electroporation
Electric pulses were delivered using an electric pulse generator (Square Wave Electroporator CUY21EDIT; Nepa Gene Co., Ltd.). Electrodes consisted of a pair of stainless steel needles of 5 mm in length and 0.4 mm in diameter, fixed with a distance (…
 
Epidermis-Targeted Gene Transfer Using In Vivo Electroporation
 
Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect
Stereomicroscopic views of transfected testes charged with various voltages and observed under visible (A) or excitation (B) light after 5 wk. Voltage is indicated on each testis (A). Loss of testicular weight 5 wk after electric charge in 12-day-old…
 
Misexpression of the gene of interest by in ovo electroporation
Mechanisms of brain regionalization and neural circuit formation have been studied by misexpression of transcription factors (En1/2, Pax2/5/6, Otx2, Gbx2), secreted factors (Fgf, Shh, semaphoring), signal transduction molecule (Ras, Sprouty2) and receptors…
 
Misexpression of the gene of interest by in ovo electroporation
Mechanisms of brain regionalization and neural circuit formation have been studied by misexpression of transcription factors (En1/2, Pax2/5/6, Otx2, Gbx2), secreted factors (Fgf, Shh, semaphoring), signal transduction molecule (Ras, Sprouty2) and receptors…
 
Knock-down by transfection of shRNA expression vector by electroporation
(B) Select target DNA sequence of 19 to 21 mer. Sense and antisense sequence were linked to a nucleotide spacer as a loop and put into expression vector that is driven by U6 or H1 promoter. Commercially available expression vector that dreives expression…
 
Method to introduce genes into epithelial cells of the chicken embryonic stomach (proventriculus)
4. Apply pulses of 30V for 15 times with pulse length of 50ms, and interval time of 75ms.  Remove agarose gel immediately and wash it in Tyrode's solution.  Then take out the tissues from the gel and wash them well in Tryode's solution.  The tissues…
 
Electroporation of Xenopus embryo: Gene Delivery into the Primordium Eye at the Neural Plate Stage
Fig.(1) shows the general setting of the electroporation. Vitelline membrane of the embryo (st.12-13) is usually kept intact. Inject 5-10nl of GFP-mRNA(1µg/µl, 0.05% Fast Green) into intercellular space of upper-few epithelial layers of the neural…
 
In vivo gene transfer into the adult honeybee brain by using electroporation
a) Schematic representation of the micropipette and electrodes placed in the honeybee brain. Purple indicates micropipette filled with DNA solution, blue indicates electrodes. AN, antennae; C, compound eyes; MB, mushroom bodies; Oc, ocelli; OL, optic…
 
Electrochemotherapy for digital chondrosarcoma
 
Electrochemotherapy for digital chondrosarcoma
 
NEPA21, CUY21SC and CUY21EDIT Multiple Applications and Electrode Recommendations
A 2 cm midline incision is then made in the abdominal wall along the linea alba using a set of forceps and scissors. A piece of sterile gauze with a hole cut in the center is placed over the incision, and one uterine horn is drawn out through the…
 
NEP21, CUY21SC and CUY21EDIT Multiple Applications and Electrode Recommendations
 
NEPA21, CUY21SC and CUY21EDIT Multiple Applications and Electrode Recommendations
A 2 cm midline incision is then made in the abdominal wall along the linea alba using a set of forceps and scissors. A piece of sterile gauze with a hole cut in the center is placed over the incision, and one uterine horn is drawn out through the hole…
 
NEPA21_Retina_EP
NEPA21 Retina EP Ex Vivo EP            
 
NEPA21_Retina_EP
(B) The current was applied with the positive electrode contralateral to the injected eye. After prior injection of plasmid DNA into the subretinal space of the right eye, this arrangement electrophoresed the negatively-charged DNA toward the RPE layer…
 
NEPA21_Retina_EP
NEPA21 Retina EP Ex Vivo EP            
 
NEPA21_Retina_EP
 
NEPA21_Retina_EP
 
NEPA21_Retina_EP
 
NEPA21_Retina_EP
(B) The current was applied with the positive electrode contralateral to the injected eye. After prior injection of plasmid DNA into the subretinal space of the right eye, this arrangement electrophoresed the negatively-charged DNA toward the RPE layer…
 
NEPA21_Retina_EP
 Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEP21, CUY21SC and CUY21EDIT Multiple Applications and Electrode Recommendations
 
LTM-1000 : Laser Thermal Microinjector
Heat-induced expansion of liquid in a sealed capillary generates high pressure, which enables injection by capillaries with the tip diameter around 0.1μm (Knoblauch et al., Nature Biotech., 1999). As the heat source, we chose a laser beam which enables…
 
LTM-1000 : Laser Thermal Microinjector
Heat-induced expansion of liquid in a sealed capillary generates high pressure, which enables injection by capillaries with the tip diameter around 0.1μm (Knoblauch et al., Nature Biotech., 1999). As the heat source, we chose a laser beam which enables…
 
LTM-1000 : Laser Thermal Microinjector
Heat-induced expansion of liquid in a sealed capillary generates high pressure, which enables injection by capillaries with the tip diameter around 0.1μm (Knoblauch et al., Nature Biotech., 1999). As the heat source, we chose a laser beam which enables…
 
NEPA21, CUY21SC and CUY21EDIT Multiple Applications and Electrode Recommendations
 
NEPA21, CUY21SC and CUY21EDIT Multiple Applications and Electrode Recommendations
A 2 cm midline incision is then made in the abdominal wall along the linea alba using a set of forceps and scissors. A piece of sterile gauze with a hole cut in the center is placed over the incision, and one uterine horn is drawn out through the…
 
Electroporation Protocols - In Vitro, In Ovo, In Utero, In Vivo, Ex Vivo and New Culture
 
In Ovo Electroporation
Some clients, however, who have to contend with a large number of samples on a daily basis find it difficult to manipulate a pair of electrodes vertically.  Such clients prefer to move the pair of electrodes horizontally.  The CUY613 series was designed…
 
Chick and Quail Embryo (in ovo) - Neural Tube, Mesencephalon, Diencephalon (HH* Stage 10
Please also note the following links and attached articles for further information on the NEPA21’s In Ovo capacity. (Please note where a reference is made in the resource material to the CUY21 systems (EDIT or SC), the NEPA21 replaces them and can…
 
Chick and Quail Embryo (in ovo) - Neural Tube, Mesencephalon, Diencephalon (HH* Stage 10
Please also note the following links and attached articles for further information on the NEPA21’s In Ovo capacity. (Please note where a reference is made in the resource material to the CUY21 systems (EDIT or SC), the NEPA21 replaces them and can…
 
Chicken and Quail Embryo (In Ovo) - Neural Tube, Mesencephalon, Diencephalon (HH* Stage 10)
Please also note the following links and attached articles for further information on the NEPA21’s In Ovo capacity. (Please note where a reference is made in the resource material to the CUY21 systems (EDIT or SC), the NEPA21 replaces them and can…
 
Mouse/Rat – Muscle
The first method involves injecting a pair of needle electrodes CUY560-5/-10 into the muscle above skin.  Since no surgery is involved, the researcher can easily perform electroporation and do so consecutively in a short period of time.  In our opinion…
 
Direct Gene Transfer into Mature Seeds via Electroporation
Fertile transgenic plants were regenerated and self-fertilized seeds were obtained in rice and wheat. Transgene integration was confirmed by Southern hybridization. Transmission of the transgene into the next generation (T1) was indicated by PCR analysis…
 
Zebrafish_Electroporation
Please note the Zebrafish video referred to above demonstrates the NEPA21 technique used in the article:  ‘Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development (Katie E. Holmes1…
 
Zebrafish_Electroporation
Please note the Zebrafish video referred to above demonstrates the NEPA21 technique used in the article:  ‘Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development (Katie E. Holmes1…
 
Cultured Mouse Embryos and Large Tissue
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe…
 
Cultured Mouse Embryos and Large Tissue
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe…
 
Cultured Mouse Embryos and Large Tissue
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe…
 
Cultured Mouse Embryos and Large Tissue
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe…
 
Cultured Mouse Embryos and Large Tissue
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe…
 
Cultured Mouse Embryos and Large Tissue
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe…
 
Mouse and Rate Tissue and Brain Slice
 
Mouse and Rate Tissue and Brain Slice
 
Cultured Mouse Embryos and Large Tissue
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe…
 
Mouse and Rate Tissue and Brain Slice
 
Mouse and Rate Tissue and Brain Slice
 
Mouse and Rate Tissue and Brain Slice
 
Mouse and Rat Tissue and Brain Slice
 
Mouse and Rat Tissue and Brain Slice
 
Mouse/Rat – Muscle
The first method involves injecting a pair of needle electrodes CUY560-5/-10 into the muscle above skin.  Since no surgery is involved, the researcher can easily perform electroporation and do so consecutively in a short period of time.  In our opinion…
 
NEPA21_Retina_EP
 Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
 Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
 Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
Please also note the following links and attached articles for further information on the NEPA21’s In Ovo capacity. (Please note where a reference is made in the resource material to the CUY21 systems (EDIT or SC), the NEPA21 replaces them and can…
 
Mouse and Rat Tissue and Brain Slice
 
Mouse/Rat – Muscle
Since no surgery is involved, the researcher can easily perform electroporation and do so consecutively in a short period of time.  In our opinion, this is the best method.  However, as the volume of muscle affects the resistance value, and thus, actual…
 
Postnatal_Cerebellum_EP_with_the_CUY699P7x6
However, our preferred and recommended electrode configuration for postnatal cerebellum electroporation does not combine three electrodes in an awkward single unit but instead uses our CUY700PL type-electrodes in combination with the following electrodes…
 
Postnatal_Cerebellum_EP_with_the_CUY699P7x6
However, our preferred and recommended electrode configuration for postnatal cerebellum electroporation does not combine three electrodes in an awkward single unit but instead uses our CUY700PL type-electrodes in combination with the following electrodes…
 
Mouse and Rat Tissue and Brain Slice
 
Mouse and Rat Tissue and Brain Slice
 
Mouse and Rat Tissue and Brain Slice
 
Mouse and Rat Tissue and Brain Slice
 
Mouse and Rat Tissue and Brain Slice
 
Mouse and Rat Tissue and Brain Slice
 
Mouse/Rat – Muscle
Since no surgery is involved, the researcher can easily perform electroporation and do so consecutively in a short period of time.  In our opinion, this is the best method.  However, as the volume of muscle affects the resistance value, and thus, actual…
 
Mouse/Rat – Muscle
Since no surgery is involved, the researcher can easily perform electroporation and do so consecutively in a short period of time.  In our opinion, this is the best method.  However, as the volume of muscle affects the resistance value, and thus, actual…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
Adult Mouse and Rat (In vivo) - Liver
Zang  et al.Inhibition  of  nuclear  delivery  of  plasmid  DNA  and  transcription  by  interferon   γ: hurdles to be overcome for sustained gene  therapy.Gene  Ther. 2011  Sep;18(9):891-7. doi: 10.1038/gt.2011.35. Epub 2011  Mar 31…
 
Rat knockout
o    Beginning with crypt isolation from a human colon sample, genetically modified organoids can be obtained in 3 week.  Isolated intestinal crypts to be expanded for over a year with the addition of essential growth factors and after embedding…
 
Adult Mouse and Rat (In vivo) - Liver
Zang  et al.Inhibition  of  nuclear  delivery  of  plasmid  DNA  and  transcription  by  interferon   γ: hurdles to be overcome for sustained gene  therapy.Gene  Ther. 2011  Sep;18(9):891-7. doi: 10.1038/gt.2011.35. Epub 2011  Mar 31…
 
Adult Mouse and Rat (In vivo) - Liver
Zang  et al.Inhibition  of  nuclear  delivery  of  plasmid  DNA  and  transcription  by  interferon   γ: hurdles to be overcome for sustained gene  therapy.Gene  Ther. 2011  Sep;18(9):891-7. doi: 10.1038/gt.2011.35. Epub 2011  Mar 31…
 
Adult Mouse and Rat (In vivo) - Liver
Zang  et al.Inhibition  of  nuclear  delivery  of  plasmid  DNA  and  transcription  by  interferon   γ: hurdles to be overcome for sustained gene  therapy.Gene  Ther. 2011  Sep;18(9):891-7. doi: 10.1038/gt.2011.35. Epub 2011  Mar 31…
 
Adult Mouse and Rat (In vivo) - Liver
Zang  et al.Inhibition  of  nuclear  delivery  of  plasmid  DNA  and  transcription  by  interferon   γ: hurdles to be overcome for sustained gene  therapy.Gene  Ther. 2011  Sep;18(9):891-7. doi: 10.1038/gt.2011.35. Epub 2011  Mar 31…
 
Rat knockout
o    Beginning with crypt isolation from a human colon sample, genetically modified organoids can be obtained in 3 week.  Isolated intestinal crypts to be expanded for over a year with the addition of essential growth factors and after embedding…
 
Zebrafish_Electroporation
Please note the Zebrafish video referred to above demonstrates the NEPA21 technique used in the article:  ‘Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development (Katie E. Holmes1…
 
Zebrafish_Electroporation
Please note the Zebrafish video referred to above demonstrates the NEPA21 technique used in the article:  ‘Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development (Katie E. Holmes1…
 
Zebrafish_Electroporation
Please note the Zebrafish video referred to above demonstrates the NEPA21 technique used in the article:  ‘Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development (Katie E. Holmes1…
 
Zebrafish_Electroporation
Please note the Zebrafish video referred to above demonstrates the NEPA21 technique used in the article:  ‘Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development (Katie E. Holmes1…
 
Zebrafish_Electroporation
Please note the Zebrafish video referred to above demonstrates the NEPA21 technique used in the article:  ‘Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development (Katie E. Holmes1…
 
Zebrafish_Electroporation
Please note the Zebrafish video referred to above demonstrates the NEPA21 technique used in the article:  ‘Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development (Katie E. Holmes1…
 
Organoid EP
 
Organoid EP
 
Adult Mouse and Rat (In vivo) - Liver
Zang  et al.Inhibition  of  nuclear  delivery  of  plasmid  DNA  and  transcription  by  interferon   γ: hurdles to be overcome for sustained gene  therapy.Gene  Ther. 2011  Sep;18(9):891-7. doi: 10.1038/gt.2011.35. Epub 2011  Mar 31…
 
Adult Mouse and Rat (In vivo) - Liver
Zang  et al.Inhibition  of  nuclear  delivery  of  plasmid  DNA  and  transcription  by  interferon   γ: hurdles to be overcome for sustained gene  therapy.Gene  Ther. 2011  Sep;18(9):891-7. doi: 10.1038/gt.2011.35. Epub 2011  Mar 31…
 
Adult Mouse and Rat (In vivo) - Liver
Zang  et al.Inhibition  of  nuclear  delivery  of  plasmid  DNA  and  transcription  by  interferon   γ: hurdles to be overcome for sustained gene  therapy.Gene  Ther. 2011  Sep;18(9):891-7. doi: 10.1038/gt.2011.35. Epub 2011  Mar 31…
 
Adult Mouse and Rat (In vivo) - Liver
Zang  et al.Inhibition  of  nuclear  delivery  of  plasmid  DNA  and  transcription  by  interferon   γ: hurdles to be overcome for sustained gene  therapy.Gene  Ther. 2011  Sep;18(9):891-7. doi: 10.1038/gt.2011.35. Epub 2011  Mar 31…
 
Adult Mouse and Rat (In vivo) - Liver
Zang  et al.Inhibition  of  nuclear  delivery  of  plasmid  DNA  and  transcription  by  interferon   γ: hurdles to be overcome for sustained gene  therapy.Gene  Ther. 2011  Sep;18(9):891-7. doi: 10.1038/gt.2011.35. Epub 2011  Mar 31…
 
Adult Mouse and Rat (In vivo) - Liver
Zang  et al.Inhibition  of  nuclear  delivery  of  plasmid  DNA  and  transcription  by  interferon   γ: hurdles to be overcome for sustained gene  therapy.Gene  Ther. 2011  Sep;18(9):891-7. doi: 10.1038/gt.2011.35. Epub 2011  Mar 31…
 
In Ovo Electroporation
Some clients, however, who have to contend with a large number of samples on a daily basis find it difficult to manipulate a pair of electrodes vertically.  Such clients prefer to move the pair of electrodes horizontally.  The CUY613 series was designed…
 
In Ovo Electroporation
Some clients, however, who have to contend with a large number of samples on a daily basis find it difficult to manipulate a pair of electrodes vertically.  Such clients prefer to move the pair of electrodes horizontally.  The CUY613 series was designed…
 
In Ovo Electroporation
Some clients, however, who have to contend with a large number of samples on a daily basis find it difficult to manipulate a pair of electrodes vertically.  Such clients prefer to move the pair of electrodes horizontally.  The CUY613 series was designed…
 
In Ovo Electroporation
Some clients, however, who have to contend with a large number of samples on a daily basis find it difficult to manipulate a pair of electrodes vertically.  Such clients prefer to move the pair of electrodes horizontally.  The CUY613 series was designed…
 
In Ovo Electroporation
Some clients, however, who have to contend with a large number of samples on a daily basis find it difficult to manipulate a pair of electrodes vertically.  Such clients prefer to move the pair of electrodes horizontally.  The CUY613 series was designed…
 
In Ovo Electroporation
Some clients, however, who have to contend with a large number of samples on a daily basis find it difficult to manipulate a pair of electrodes vertically.  Such clients prefer to move the pair of electrodes horizontally.  The CUY613 series was designed…
 
In Ovo Electroporation
Some clients, however, who have to contend with a large number of samples on a daily basis find it difficult to manipulate a pair of electrodes vertically.  Such clients prefer to move the pair of electrodes horizontally.  The CUY613 series was designed…
 
In Ovo Electroporation
Some clients, however, who have to contend with a large number of samples on a daily basis find it difficult to manipulate a pair of electrodes vertically.  Such clients prefer to move the pair of electrodes horizontally.  The CUY613 series was designed…
 
In Ovo Electroporation
Some clients, however, who have to contend with a large number of samples on a daily basis find it difficult to manipulate a pair of electrodes vertically.  Such clients prefer to move the pair of electrodes horizontally.  The CUY613 series was designed…
 
In Ovo Electroporation
Some clients, however, who have to contend with a large number of samples on a daily basis find it difficult to manipulate a pair of electrodes vertically.  Such clients prefer to move the pair of electrodes horizontally.  The CUY613 series was designed…
 
In Ovo Electroporation
Some clients, however, who have to contend with a large number of samples on a daily basis find it difficult to manipulate a pair of electrodes vertically.  Such clients prefer to move the pair of electrodes horizontally.  The CUY613 series was designed…
 
In Ovo Electroporation
Some clients, however, who have to contend with a large number of samples on a daily basis find it difficult to manipulate a pair of electrodes vertically.  Such clients prefer to move the pair of electrodes horizontally.  The CUY613 series was designed…
 
In Ovo Electroporation
Some clients, however, who have to contend with a large number of samples on a daily basis find it difficult to manipulate a pair of electrodes vertically.  Such clients prefer to move the pair of electrodes horizontally.  The CUY613 series was designed…
 
Cultured Mouse Embryos and Large Tissue
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe…
 
Cultured Mouse Embryos and Large Tissue
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe…
 
Cultured Mouse Embryos and Large Tissue
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe…
 
Mouse and Rat Tissue and Brain Slice
 
Mouse and Rat Tissue and Brain Slice
 
Adult Mouse and Rat (In vivo) - Liver
Zang  et al.Inhibition  of  nuclear  delivery  of  plasmid  DNA  and  transcription  by  interferon   γ: hurdles to be overcome for sustained gene  therapy.Gene  Ther. 2011  Sep;18(9):891-7. doi: 10.1038/gt.2011.35. Epub 2011  Mar 31…
 
Adult Mouse and Rat (In vivo) - Liver
Zang  et al.Inhibition  of  nuclear  delivery  of  plasmid  DNA  and  transcription  by  interferon   γ: hurdles to be overcome for sustained gene  therapy.Gene  Ther. 2011  Sep;18(9):891-7. doi: 10.1038/gt.2011.35. Epub 2011  Mar 31…
 
Adult Mouse and Rat (In vivo) - Liver
Zang  et al.Inhibition  of  nuclear  delivery  of  plasmid  DNA  and  transcription  by  interferon   γ: hurdles to be overcome for sustained gene  therapy.Gene  Ther. 2011  Sep;18(9):891-7. doi: 10.1038/gt.2011.35. Epub 2011  Mar 31…
 
Adult Mouse and Rat (In vivo) - Liver
Zang  et al.Inhibition  of  nuclear  delivery  of  plasmid  DNA  and  transcription  by  interferon   γ: hurdles to be overcome for sustained gene  therapy.Gene  Ther. 2011  Sep;18(9):891-7. doi: 10.1038/gt.2011.35. Epub 2011  Mar 31…
 
Adult Mouse and Rat (In vivo) - Liver
Zang  et al.Inhibition  of  nuclear  delivery  of  plasmid  DNA  and  transcription  by  interferon   γ: hurdles to be overcome for sustained gene  therapy.Gene  Ther. 2011  Sep;18(9):891-7. doi: 10.1038/gt.2011.35. Epub 2011  Mar 31…
 
Adult Mouse and Rat (In vivo) - Liver
Zang  et al.Inhibition  of  nuclear  delivery  of  plasmid  DNA  and  transcription  by  interferon   γ: hurdles to be overcome for sustained gene  therapy.Gene  Ther. 2011  Sep;18(9):891-7. doi: 10.1038/gt.2011.35. Epub 2011  Mar 31…
 
Please also note the following links and attached articles for further information on the NEPA21’s In Ovo capacity. (Please note where a reference is made in the resource material to the CUY21 systems (EDIT or SC), the NEPA21 replaces them and can…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
Organoid EP
 
Organoid EP
 
Organoid EP
o    Beginning with crypt isolation from a human colon sample, genetically modified organoids can be obtained in 3 week.  Isolated intestinal crypts to be expanded for over a year with the addition of essential growth factors and after embedding…
 
Organoid EP
o    Beginning with crypt isolation from a human colon sample, genetically modified organoids can be obtained in 3 week.  Isolated intestinal crypts to be expanded for over a year with the addition of essential growth factors and after embedding…
 
Organoid EP
o    Beginning with crypt isolation from a human colon sample, genetically modified organoids can be obtained in 3 week.  Isolated intestinal crypts to be expanded for over a year with the addition of essential growth factors and after embedding…
 
Organoid EP
o    Beginning with crypt isolation from a human colon sample, genetically modified organoids can be obtained in 3 week.  Isolated intestinal crypts to be expanded for over a year with the addition of essential growth factors and after embedding…
 
Organoid EP
o    Beginning with crypt isolation from a human colon sample, genetically modified organoids can be obtained in 3 week.  Isolated intestinal crypts to be expanded for over a year with the addition of essential growth factors and after embedding…
 
Organoid EP
o    Beginning with crypt isolation from a human colon sample, genetically modified organoids can be obtained in 3 week.  Isolated intestinal crypts to be expanded for over a year with the addition of essential growth factors and after embedding…
 
Mouse and Rat Tissue and Brain Slice
 
Zebrafish_Electroporation
Please note the Zebrafish video referred to above demonstrates the NEPA21 technique used in the article:  ‘Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development (Katie E. Holmes1…
 
Zebrafish_Electroporation
Please note the Zebrafish video referred to above demonstrates the NEPA21 technique used in the article:  ‘Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development (Katie E. Holmes1…
 
Organoid EP
o    Beginning with crypt isolation from a human colon sample, genetically modified organoids can be obtained in 3 week.  Isolated intestinal crypts to be expanded for over a year with the addition of essential growth factors and after embedding…
 
Organoid EP
o    Beginning with crypt isolation from a human colon sample, genetically modified organoids can be obtained in 3 week.  Isolated intestinal crypts to be expanded for over a year with the addition of essential growth factors and after embedding…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
NEPA21_Retina_EP
Arrangement of electrodes for in vivo electroporation for RPE transfection.(A) Tweezer-type electrodes were placed on the corneal surface of either eye of a 1-month-old Sprague-Dawley rat.(B) The current was applied with the positive electrode contralateral…
 
Postnatal_Cerebellum_EP_with_the_CUY699P7x6
However, our preferred and recommended electrode configuration for postnatal cerebellum electroporation does not combine three electrodes in an awkward single unit but instead uses our CUY700PL type-electrodes in combination with the following electrodes…
 
Postnatal_Cerebellum_EP_with_the_CUY699P7x6
However, our preferred and recommended electrode configuration for postnatal cerebellum electroporation does not combine three electrodes in an awkward single unit but instead uses our CUY700PL type-electrodes in combination with the following electrodes…
 
Postnatal_Cerebellum_EP_with_the_CUY699P7x6
However, our preferred and recommended electrode configuration for postnatal cerebellum electroporation does not combine three electrodes in an awkward single unit but instead uses our CUY700PL type-electrodes in combination with the following electrodes…
 
Postnatal_Cerebellum_EP_with_the_CUY699P7x6
However, our preferred and recommended electrode configuration for postnatal cerebellum electroporation does not combine three electrodes in an awkward single unit but instead uses our CUY700PL type-electrodes in combination with the following electrodes…
 
Postnatal_Cerebellum_EP_with_the_CUY699P7x6
However, our preferred and recommended electrode configuration for postnatal cerebellum electroporation does not combine three electrodes in an awkward single unit but instead uses our CUY700PL type-electrodes in combination with the following electrodes…
 
Postnatal_Cerebellum_EP_with_the_CUY699P7x6
However, our preferred and recommended electrode configuration for postnatal cerebellum electroporation does not combine three electrodes in an awkward single unit but instead uses our CUY700PL type-electrodes in combination with the following electrodes…
 
Postnatal_Cerebellum_EP_with_the_CUY699P7x6
However, our preferred and recommended electrode configuration for postnatal cerebellum electroporation does not combine three electrodes in an awkward single unit but instead uses our CUY700PL type-electrodes in combination with the following electrodes…
 
Postnatal_Cerebellum_EP_with_the_CUY699P7x6
However, our preferred and recommended electrode configuration for postnatal cerebellum electroporation does not combine three electrodes in an awkward single unit but instead uses our CUY700PL type-electrodes in combination with the following electrodes…
 
Postnatal_Cerebellum_EP_with_the_CUY699P7x6
High-performance and reliable site-directed in vivo genetic manipulation of mouse and rat brain by in utero electroporation with a triple-electrode probe, Joanna Szczurkowska,  Andrzej W. Cwetsch,  Marco dal Maschio,  Diego Ghezzi,  Gian Michele…
 
Postnatal_Cerebellum_EP_with_the_CUY699P7x6
However, our preferred and recommended electrode configuration for postnatal cerebellum electroporation does not combine three electrodes in an awkward single unit but instead uses our CUY700P_L type-electrodes in combination with the following electrodes…
 
Postnatal_Cerebellum_EP_with_the_CUY699P7x6
However, our preferred and recommended electrode configuration for postnatal cerebellum electroporation does not combine three electrodes in an awkward single unit but instead uses our CUY700PL type-electrodes in combination with the following electrodes…
 
Postnatal_Cerebellum_EP_with_the_CUY699P7x6
However, our preferred and recommended electrode configuration for postnatal cerebellum electroporation does not combine three electrodes in an awkward single unit but instead uses our CUY700PL type-electrodes in combination with the following electrodes…
 
Postnatal_Cerebellum_EP_with_the_CUY699P7x6
However, our preferred and recommended electrode configuration for postnatal cerebellum electroporation does not combine three electrodes in an awkward single unit but instead uses our CUY700P_L type-electrodes in combination with the following electrodes…
 
Postnatal_Cerebellum_EP_with_the_CUY699P7x6
However, our preferred and recommended electrode configuration for postnatal cerebellum electroporation does not combine three electrodes in an awkward single unit but instead uses our CUY700P_L type-electrodes in combination with the following electrodes…
 
Rat knockout
o    Beginning with crypt isolation from a human colon sample, genetically modified organoids can be obtained in 3 week.  Isolated intestinal crypts to be expanded for over a year with the addition of essential growth factors and after embedding…
 
Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production
Compared to other devices on the market, the NEPA21 system offers the researcher a level of previously unavailable control over energy delivery to the electroporation target.  This control is generated via unique electroporation pulse-output configurations…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21_Retina_EP
(B) The current was applied with the positive electrode contralateral to the injected eye. After prior injection of plasmid DNA into the subretinal space of the right eye, this arrangement electrophoresed the negatively-charged DNA toward the RPE layer…
 
NEPA21_Retina_EP
(B) The current was applied with the positive electrode contralateral to the injected eye. After prior injection of plasmid DNA into the subretinal space of the right eye, this arrangement electrophoresed the negatively-charged DNA toward the RPE layer…
 
NEPA21_Retina_EP
(B) The current was applied with the positive electrode contralateral to the injected eye. After prior injection of plasmid DNA into the subretinal space of the right eye, this arrangement electrophoresed the negatively-charged DNA toward the RPE layer…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production
Compared to other devices on the market, the NEPA21 system offers the researcher a level of previously unavailable control over energy delivery to the electroporation target.  This control is generated via unique electroporation pulse-output configurations…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
test 1
Yuka Nakazawa, Yuichiro Hara, Yasuyoshi Oka, Okiru Komine, Diana van den Heuvel 3, Chaowan Guo 1, Yasukazu Daigaku 4, Mayu Isono, Yuxi He, Mayuko Shimada, Kana Kato, Nan Jia, Satoru Hashimoto, Yuko Kotani, Yuka Miyoshi, Miyako Tanaka, Akira Sobue, Norisato…
 
Yuka Nakazawa, Yuichiro Hara, Yasuyoshi Oka, Okiru Komine, Diana van den Heuvel 3, Chaowan Guo 1, Yasukazu Daigaku 4, Mayu Isono, Yuxi He, Mayuko Shimada, Kana Kato, Nan Jia, Satoru Hashimoto, Yuko Kotani, Yuka Miyoshi, Miyako Tanaka, Akira Sobue, Norisato…
 
test 1
Yuka Nakazawa, Yuichiro Hara, Yasuyoshi Oka, Okiru Komine, Diana van den Heuvel 3, Chaowan Guo 1, Yasukazu Daigaku 4, Mayu Isono, Yuxi He, Mayuko Shimada, Kana Kato, Nan Jia, Satoru Hashimoto, Yuko Kotani, Yuka Miyoshi, Miyako Tanaka, Akira Sobue, Norisato…
 
Yuka Nakazawa, Yuichiro Hara, Yasuyoshi Oka, Okiru Komine, Diana van den Heuvel 3, Chaowan Guo 1, Yasukazu Daigaku 4, Mayu Isono, Yuxi He, Mayuko Shimada, Kana Kato, Nan Jia, Satoru Hashimoto, Yuko Kotani, Yuka Miyoshi, Miyako Tanaka, Akira Sobue, Norisato…
 
Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production
Compared to other devices on the market, the NEPA21 system offers the researcher a level of previously unavailable control over energy delivery to the electroporation target.  This control is generated via unique electroporation pulse-output configurations…
 
Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production
Compared to other devices on the market, the NEPA21 system offers the researcher a level of previously unavailable control over energy delivery to the electroporation target.  This control is generated via unique electroporation pulse-output configurations…
 
Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production
Compared to other devices on the market, the NEPA21 system offers the researcher a level of previously unavailable control over energy delivery to the electroporation target.  This control is generated via unique electroporation pulse-output configurations…
 
Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production
Compared to other devices on the market, the NEPA21 system offers the researcher a level of previously unavailable control over energy delivery to the electroporation target.  This control is generated via unique electroporation pulse-output configurations…
 
Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production
Compared to other devices on the market, the NEPA21 system offers the researcher a level of previously unavailable control over energy delivery to the electroporation target.  This control is generated via unique electroporation pulse-output configurations…
 
Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production
Compared to other devices on the market, the NEPA21 system offers the researcher a level of previously unavailable control over energy delivery to the electroporation target.  This control is generated via unique electroporation pulse-output configurations…
 
Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production
Compared to other devices on the market, the NEPA21 system offers the researcher a level of previously unavailable control over energy delivery to the electroporation target.  This control is generated via unique electroporation pulse-output configurations…
 
Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production
Compared to other devices on the market, the NEPA21 system offers the researcher a level of previously unavailable control over energy delivery to the electroporation target.  This control is generated via unique electroporation pulse-output configurations…
 
Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production
Compared to other devices on the market, the NEPA21 system offers the researcher a level of previously unavailable control over energy delivery to the electroporation target.  This control is generated via unique electroporation pulse-output configurations…
 
Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production
Compared to other devices on the market, the NEPA21 system offers the researcher a level of previously unavailable control over energy delivery to the electroporation target.  This control is generated via unique electroporation pulse-output configurations…
 
Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production
Compared to other devices on the market, the NEPA21 system offers the researcher a level of previously unavailable control over energy delivery to the electroporation target.  This control is generated via unique electroporation pulse-output configurations…
 
Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production
Compared to other devices on the market, the NEPA21 system offers the researcher a level of previously unavailable control over energy delivery to the electroporation target.  This control is generated via unique electroporation pulse-output configurations…
 
Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production
Compared to other devices on the market, the NEPA21 system offers the researcher a level of previously unavailable control over energy delivery to the electroporation target.  This control is generated via unique electroporation pulse-output configurations…
 
Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production
Compared to other devices on the market, the NEPA21 system offers the researcher a level of previously unavailable control over energy delivery to the electroporation target.  This control is generated via unique electroporation pulse-output configurations…
 
Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production
Compared to other devices on the market, the NEPA21 system offers the researcher a level of previously unavailable control over energy delivery to the electroporation target.  This control is generated via unique electroporation pulse-output configurations…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21 Publications
Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourqui…
 
NEPA21_Retina_EP
(B) The current was applied with the positive electrode contralateral to the injected eye. After prior injection of plasmid DNA into the subretinal space of the right eye, this arrangement electrophoresed the negatively-charged DNA toward the RPE layer…
 
NEPA21_Retina_EP
(B) The current was applied with the positive electrode contralateral to the injected eye. After prior injection of plasmid DNA into the subretinal space of the right eye, this arrangement electrophoresed the negatively-charged DNA toward the RPE layer…
 
NEPA21 Publications
bioRxiv February 25, 2020Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publications
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publications
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publications
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publications
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Pub new
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publication List
Stem Cells Transl Med. 2021 Jan;10(1):115-127.Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Test spacing
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
NEPA21 Publication List
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Test spacing
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Test spacing
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Pub new
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Pub new
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Pub new
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Pub new
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Test spacing
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Test spacing
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Pub new
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Pub new
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Pub new
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Pub new
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Pub new
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Pub new
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Pub new
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Pub new
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Pub new
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Pub new
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Pub new
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Pub new
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Pub new
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Pub new
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Pub new
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Pub new
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Test spacing
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Test spacing
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Test spacing
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Test spacing
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Test spacing
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Test spacing
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Test spacing
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Test spacing
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Test spacing
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Test spacing
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Test spacing
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Test spacing
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Test spacing
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 
Test spacing
Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita…
 


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